干旱区生态环境知识资源中心(Arid): Evaluation of the anticancer activity and fatty acids composition of "Handal" (Citrullus colocynthis L.) seed oil, a desert plant from south Jordan

Arid

DOI	10.1002/fsn3.1994
	Evaluation of the anticancer activity and fatty acids composition of "Handal" (Citrullus colocynthis L.) seed oil, a desert plant from south Jordan
	Al-Hwaiti, Mohammad S.; Alsbou, Eid M.; Abu Sheikha, Ghassan; Bakchiche, Boulanouar; Pham, Thu Huong; Thomas, Raymond H.; Bardaweel, Sanaa K.
通讯作者	Bardaweel, SK
来源期刊	FOOD SCIENCE & NUTRITION
ISSN	2048-7177
英文摘要	Background The chemical composition of Handal (Citrullus colocynthis L.) seed oil cultivated in Jordan deserts was characterized, and its bioactivity was evaluated. Methods The oil was extracted from the grinded seeds in 500 ml Soxhlet extractor for 24 hr using n-hexane, and the recovered fatty acids were methylated with methanolic-HCL. The fatty acid methyl esters (FAMEs) composition was analyzed using GC-MS and GC-FID. The anticancer activity associated with the oil was assessed against colon cancer cell lines (Caco-2 and HCT-116) and compared to its cytotoxicity on the human skin fibroblast. Multivariate analysis was used to determine relationship of the fatty acid composition with that of the anticancer activity. Results The results demonstrated that fatty acid composition of Citrullus colocynthis seed oil chiefly contains Linoleic acid, denoted as C18:2n6 (75%), followed by Palmitic acid C16:0 (8%), Stearic acid C18:0 (5%), and Oleic acid C18:1n9 (9%). It is demonstrated as an excellent source of essential fatty acids omega-6 (e.g., Linoleic acid), whereas omega-3 (e.g., alpha-Linolenic acid) and hydroxy polyunsaturated fatty acids are found at small level. Interestingly, the oil exhibited reasonable anticancer effects against colorectal cancer cell lines with IC50 values varying between 4 and 7 mg/ml. The correlation test revealed a relationship between the fatty acid composition and the effectiveness on treatments. Conclusions Handal plant from Jordan appears to have very high level of Linoleic acid compared to other oils measured in different geographic locations and that there appears to be some anticancer activities associated with the fatty acid content of Handal seed oil.
英文关键词	anticancer Citrullus colocynthis fatty acids gas chromatography hanadal seed oil
类型	Article ; Early Access
语种	英语
开放获取类型	DOAJ Gold
收录类别	SCI-E
WOS记录号	WOS:000587748000001
WOS关键词	ETHNOPHARMACOLOGICAL SURVEY ; ANTIOXIDANT ; REGION ; SCHRAD ; FRUIT
WOS类目	Food Science & Technology
WOS研究方向	Food Science & Technology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/328460
作者单位	[Al-Hwaiti, Mohammad S.] Al Hussein Bin Talal Univ, Fac Engn, Dept Environm Engn, Maan, Jordan; [Alsbou, Eid M.] Al Hussein Bin Talal Univ, Chem Dept, Fac Sci, Maan, Jordan; [Abu Sheikha, Ghassan] Al Zaytoonah Univ Jordan, Dept Pharm, Maan, Jordan; [Bakchiche, Boulanouar] Laghouat Univ, Fac Technol, Lab Proc Engn, Laghouat, Algeria; [Pham, Thu Huong; Thomas, Raymond H.] Mem Univ Newfoundland, Boreal Ecosyst Res Facil, Sch Sci & Environm, Grenfell Campus, Corner Brook, NF, Canada; [Bardaweel, Sanaa K.] Univ Jordan, Sch Pharm, Dept Pharmaceut Sci, Amman 11942, Jordan
推荐引用方式 GB/T 7714	Al-Hwaiti, Mohammad S.,Alsbou, Eid M.,Abu Sheikha, Ghassan,et al. Evaluation of the anticancer activity and fatty acids composition of "Handal" (Citrullus colocynthis L.) seed oil, a desert plant from south Jordan[J].
APA	Al-Hwaiti, Mohammad S..,Alsbou, Eid M..,Abu Sheikha, Ghassan.,Bakchiche, Boulanouar.,Pham, Thu Huong.,...&Bardaweel, Sanaa K..
MLA	Al-Hwaiti, Mohammad S.,et al."Evaluation of the anticancer activity and fatty acids composition of "Handal" (Citrullus colocynthis L.) seed oil, a desert plant from south Jordan".FOOD SCIENCE & NUTRITION