干旱区生态环境知识资源中心(Arid): Antarctic desert soil bacteria exhibit high novel natural product potential, evaluated through long-read genome sequencing and comparative genomics

Arid

DOI	10.1111/1462-2920.15300
	Antarctic desert soil bacteria exhibit high novel natural product potential, evaluated through long-read genome sequencing and comparative genomics
	Benaud, Nicole; Edwards, Richard J.; Amos, Timothy G.; D'Agostino, Paul M.; Gutierrez-Chavez, Carolina; Montgomery, Kate; Nicetic, Iskra; Ferrari, Belinda C.
通讯作者	Ferrari, BC
来源期刊	ENVIRONMENTAL MICROBIOLOGY
ISSN	1462-2912
EISSN	1462-2920
英文摘要	Actinobacteria and Proteobacteria are important producers of bioactive natural products (NP), and these phyla dominate in the arid soils of Antarctica, where metabolic adaptations influence survival under harsh conditions. Biosynthetic gene clusters (BGCs) which encode NPs, are typically long and repetitious high G + C regions difficult to sequence with short-read technologies. We sequenced 17 Antarctic soil bacteria from multi-genome libraries, employing the long-read PacBio platform, to optimize capture of BGCs and to facilitate a comprehensive analysis of their NP capacity. We report 13 complete bacterial genomes of high quality and contiguity, representing 10 different cold-adapted genera including novel species. Antarctic BGCs exhibited low similarity to known compound BGCs (av. 31%), with an abundance of terpene, non-ribosomal peptide and polyketide-encoding clusters. Comparative genome analysis was used to map BGC variation between closely related strains from geographically distant environments. Results showed the greatest biosynthetic differences to be in a psychrotolerant Streptomyces strain, as well as a rare Actinobacteria genus, Kribbella, while two other Streptomyces spp. were surprisingly similar to known genomes. Streptomyces and Kribbella BGCs were predicted to encode antitumour, antifungal, antibacterial and biosurfactant-like compounds, and the synthesis of NPs with antibacterial, antifungal and surfactant properties was confirmed through bioactivity assays.
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000587703900001
WOS关键词	NONRIBOSOMAL PEPTIDE ; GENE CLUSTERS ; POLYKETIDE SYNTHASES ; STREPTOMYCES ; BIOSYNTHESIS ; DISCOVERY ; IDENTIFICATION ; SESQUITERPENE ; ENVIRONMENTS ; ACTINOMYCETE
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/328459
作者单位	[Benaud, Nicole; Edwards, Richard J.; Amos, Timothy G.; Gutierrez-Chavez, Carolina; Montgomery, Kate; Nicetic, Iskra; Ferrari, Belinda C.] UNSW Sydney, Sch Biotechnol & Biomol Sci, Sydney, NSW 2052, Australia; [D'Agostino, Paul M.] Tech Univ Dresden, Chair Tech Biochem, Bergstr 66, D-01602 Dresden, Germany
推荐引用方式 GB/T 7714	Benaud, Nicole,Edwards, Richard J.,Amos, Timothy G.,et al. Antarctic desert soil bacteria exhibit high novel natural product potential, evaluated through long-read genome sequencing and comparative genomics[J].
APA	Benaud, Nicole.,Edwards, Richard J..,Amos, Timothy G..,D'Agostino, Paul M..,Gutierrez-Chavez, Carolina.,...&Ferrari, Belinda C..
MLA	Benaud, Nicole,et al."Antarctic desert soil bacteria exhibit high novel natural product potential, evaluated through long-read genome sequencing and comparative genomics".ENVIRONMENTAL MICROBIOLOGY