干旱区生态环境知识资源中心(Arid): Phylogenetic diversity analysis reveals Bradyrhizobium yuanmingense and Ensifer aridi as major symbionts of mung bean (Vigna radiata L.) in Pakistan

Arid

DOI	10.1007/s42770-020-00397-9
	Phylogenetic diversity analysis reveals Bradyrhizobium yuanmingense and Ensifer aridi as major symbionts of mung bean (Vigna radiata L.) in Pakistan
	Hakim, Sughra; Imran, Asma; Mirza, M. Sajjad
通讯作者	Mirza, MS
来源期刊	BRAZILIAN JOURNAL OF MICROBIOLOGY
ISSN	1517-8382
EISSN	1678-4405
英文摘要	The present study was carried out to evaluate the diversity of rhizobia associated with nodules of mung bean in Pakistan, because this information is necessary for inoculum development. Based on sequence analysis of 16S rRNA gene of thirty-one bacteria, 11 were assigned to genus Bradyrhizobium, 17 to Ensifer, and 3 to Rhizobium. Phylogenetic analyses on the basis of 16S-23S ITS region, atpD, recA, nifH, and nodA of representative strains revealed that B. yuanmingense is the predominant species distributed throughout different mung bean-growing areas. Among the fast-growing rhizobia, Ensifer aridi was predominant in Faisalabad, Layyah, and Rawalpindi, while E. meliloti in Thal desert. Sequence variations and phylogeny of nifH and nodA genes suggested that these genes might have been co-evolved with the housekeeping genes and maintained by vertical gene transfer in rhizobia detected in the present study. Host infectivity assay revealed the successful nodulation of host by rhizobia related to genera Bradyrhizobium, Ensifer and Rhizobium. Among all, Bradyrhizobium and Ensifer spp. inoculation exhibited a significantly higher number of nodules (11-34 nodules plant(-1)) and nitrogenase activity (nodule ARA 60-110 mu mol g(-1) h(-1)). Contrary to the previous studies, our data reveal that B. yuanmingense and E. aridi are predominant species forming effective nodules in mung bean in Pakistan. Furthermore, to the best of our knowledge, this is the first report showing the effective symbiosis of E. aridi, E. meliloti, and Rhizobium pusense with mung bean. The diversity of rhizobia in different habitats revealed in the present study will contribute towards designing site-specific inocula for mung bean.
英文关键词	E meliloti Housekeeping genes Intraspecies polymorphism Predominant species Rhizobia Site-specific inocula
类型	Article ; Early Access
语种	英语
开放获取类型	DOAJ Gold
收录类别	SCI-E
WOS记录号	WOS:000584985600001
WOS关键词	MULTILOCUS SEQUENCE-ANALYSIS ; INTERNAL TRANSCRIBED SPACER ; 16S RIBOSOMAL-RNA ; MOLECULAR CHARACTERIZATION ; PHASEOLUS-VULGARIS ; NIFH GENES ; RHIZOBIA ; STRAINS ; LEGUMES ; REGIONS
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/328432
作者单位	[Hakim, Sughra; Imran, Asma; Mirza, M. Sajjad] Natl Inst Biotechnol & Genet Engn NIBGE, POB 577, Faisalabad, Pakistan; [Hakim, Sughra] Pakistan Inst Engn & Appl Sci PIEAS, Islamabad, Pakistan
推荐引用方式 GB/T 7714	Hakim, Sughra,Imran, Asma,Mirza, M. Sajjad. Phylogenetic diversity analysis reveals Bradyrhizobium yuanmingense and Ensifer aridi as major symbionts of mung bean (Vigna radiata L.) in Pakistan[J].
APA	Hakim, Sughra,Imran, Asma,&Mirza, M. Sajjad.
MLA	Hakim, Sughra,et al."Phylogenetic diversity analysis reveals Bradyrhizobium yuanmingense and Ensifer aridi as major symbionts of mung bean (Vigna radiata L.) in Pakistan".BRAZILIAN JOURNAL OF MICROBIOLOGY