干旱区生态环境知识资源中心(Arid): Optimization of in vitro germination and cryopreservation conditions for preserving date palm pollen in the USDA National Plant Germplasm System

Arid

DOI	10.1007/s11240-020-01907-1
	Optimization of in vitro germination and cryopreservation conditions for preserving date palm pollen in the USDA National Plant Germplasm System
	Araujo de Oliveira, Annie Carolina; Ledo, Ana da Silva; Polek, MaryLou; Krueger, Robert; Shepherd, Ashley; Volk, Gayle M.
通讯作者	de Oliveira, ACA
来源期刊	PLANT CELL TISSUE AND ORGAN CULTURE
ISSN	0167-6857
EISSN	1573-5044
英文摘要	Date palm (Phoenix dactylifera L.) is a staple crop across arid and semi-arid regions around the world. Both genebanks and date palm breeders benefit by having clearly defined methods for the long-term preservation of pollen. Methods were developed to cryopreserve pollen from date palm trees in the USDA-ARS National Plant Germplasm System. First, in vitro pollen germination conditions were optimized using five cultivars. Then, date palm pollen equilibrated over saturated salt solutions of Ca(NO3)(2) or MgCl2 at 23 degrees C (46% and 33% RH, respectively) or in a room set at 23% RH and 5 degrees C. Pollen was kept at these conditions for one to 56 days and then placed into liquid nitrogen vapor (LNV). Viability was assessed for fresh, moisture-equilibrated (non-LNV and LNV-exposed), and pollen stored in LNV for 9 months. The highest level of in vitro pollen germination was observed for date palm cultivar Thoory BC3 (60 +/- 2%) when it was rehydrated for 2 h and then incubated at 23 degrees C on Marquard medium with 15% sucrose. Viability remained stable for pollen that was stored in LNV for 9 months. In addition, pollen also remained viable if it was cooled to and warmed from LNV for up to 60 cycles. These data suggest that date palm pollen can be successfully placed in genebank cryostorage. Key message Using optimized in vitro germination conditions, date palm pollen can survive at least 9 months in LNV and can be cooled to and warmed from LNV at least 60 times.
英文关键词	Phoenix dactylifera L Liquid nitrogen In vitro germination
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000584591100001
WOS关键词	TUBE GROWTH ; STORAGE ; PINEAPPLE ; VIABILITY ; GRAINS
WOS类目	Biotechnology & Applied Microbiology ; Plant Sciences
WOS研究方向	Biotechnology & Applied Microbiology ; Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/328424
作者单位	[Araujo de Oliveira, Annie Carolina] Univ Fed Sergipe, Marechal Rondon Ave, BR-49100000 Sao Cristovao, SE, Brazil; [Ledo, Ana da Silva] Embrapa Coastal Tablelands, 3250 Beira Mar Ave, BR-49040490 Aracaju, SE, Brazil; [Polek, MaryLou; Krueger, Robert] USDA ARS, Natl Clonal Germplasm Repository Citrus & Dates, 1060 Martin Luther King Blvd, Riverside, CA 92507 USA; [Shepherd, Ashley; Volk, Gayle M.] USDA ARS, Natl Lab Genet Resources Preservat, 1111 S Mason St, Ft Collins, CO 80521 USA
推荐引用方式 GB/T 7714	Araujo de Oliveira, Annie Carolina,Ledo, Ana da Silva,Polek, MaryLou,et al. Optimization of in vitro germination and cryopreservation conditions for preserving date palm pollen in the USDA National Plant Germplasm System[J].
APA	Araujo de Oliveira, Annie Carolina,Ledo, Ana da Silva,Polek, MaryLou,Krueger, Robert,Shepherd, Ashley,&Volk, Gayle M..
MLA	Araujo de Oliveira, Annie Carolina,et al."Optimization of in vitro germination and cryopreservation conditions for preserving date palm pollen in the USDA National Plant Germplasm System".PLANT CELL TISSUE AND ORGAN CULTURE