干旱区生态环境知识资源中心(Arid): Humoral antibody response of 10 horses after vaccination against African horse sickness with an inactivated vaccine containing all 9 serotypes in one injection

Arid

DOI	10.1111/evj.13363
	Humoral antibody response of 10 horses after vaccination against African horse sickness with an inactivated vaccine containing all 9 serotypes in one injection
	Wernery, Ulrich; Rodriguez, Marina; Raghavan, Rekha; Syriac, Ginu; Thomas, Shruti Miriam M.; Elizabeth, Shyna K.; Federico Ronchi, Gaetano; Muhammed, Rubeena; Patteril, Nissy A.; Joseph, Sunitha
通讯作者	Wernery, U
来源期刊	EQUINE VETERINARY JOURNAL
ISSN	0425-1644
EISSN	2042-3306
英文摘要	Background African horse sickness (AHS) is a devastating viral disease of equids that was first recorded in 1327. Currently, prevention and control of the disease are based on attenuated vaccines and midge control. It has been shown that attenuated Orbivirus vaccines are not always safe as they may reverse to virulence. Objectives In the Emirate of Dubai, a vaccination experiment was carried out with an inactivated AHS vaccine produced at the Central Veterinary Research Laboratory (CVRL), Dubai, UAE to investigate the humoral antibody response of AHS-naive horses to this vaccine. Our vaccination experiment was performed to establish an AHS vaccine bank in the UAE to protect horses from the disease in case of an outbreak. Therefore, CVRL established an inactivated AHS vaccine containing all nine serotypes which induce high neutralising antibodies. Study design A total of 10 horses kept in a desert isolation area were subcutaneously and intramuscularly vaccinated with an inactivated vaccine containing all nine AHS serotypes previously isolated from Kenyan horse fatalities. Primary immunisation was followed by two booster immunisations 4 weeks and 6 months apart. After 13 months, an annual booster was administered. Methods Blood samples were regularly withdrawn for ELISA and virus neutralisation testing. Additionally, EDTA blood was tested every second day for 14 days post each vaccination for the presence of AHS virus or its RNA. Results Results show that ELISA and virus neutralising antibodies appeared after the first booster, declined after 4-6 months and therefore three vaccinations and an annual vaccination are necessary to achieve high protective virus neutralising antibodies. Main limitations No challenge infection was carried out due to the lack of a safe facility in the UAE. Conclusion Before more advanced AHS vaccines become a reality, inactivated vaccines containing all nine serotypes should be used as they produce high ELISA and neutralising antibodies.
英文关键词	horse African horse sickness immune response inactivated vaccine with 9 serotypes
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000582773300001
WOS关键词	VIRUS ; PROTEINS ; ELISA ; LIVE ; VP2
WOS类目	Veterinary Sciences
WOS研究方向	Veterinary Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/328389
作者单位	[Wernery, Ulrich; Rodriguez, Marina; Raghavan, Rekha; Syriac, Ginu; Thomas, Shruti Miriam M.; Elizabeth, Shyna K.; Muhammed, Rubeena; Patteril, Nissy A.; Joseph, Sunitha] Cent Vet Res Lab, Dubai, U Arab Emirates; [Federico Ronchi, Gaetano] Ist Zooprofilatt Sperimentale Abruzz & Molise G C, Teramo, Italy
推荐引用方式 GB/T 7714	Wernery, Ulrich,Rodriguez, Marina,Raghavan, Rekha,et al. Humoral antibody response of 10 horses after vaccination against African horse sickness with an inactivated vaccine containing all 9 serotypes in one injection[J].
APA	Wernery, Ulrich.,Rodriguez, Marina.,Raghavan, Rekha.,Syriac, Ginu.,Thomas, Shruti Miriam M..,...&Joseph, Sunitha.
MLA	Wernery, Ulrich,et al."Humoral antibody response of 10 horses after vaccination against African horse sickness with an inactivated vaccine containing all 9 serotypes in one injection".EQUINE VETERINARY JOURNAL