干旱区生态环境知识资源中心(Arid): Revealing hidden plant diversity in arid environments

Arid

DOI	10.1111/ecog.05100
	Revealing hidden plant diversity in arid environments
	Carrasco-Puga, Gabriela; Diaz, Francisca P.; Soto, Daniela C.; Hernandez-Castro, Catalina; Contreras-Lopez, Orlando; Maldonado, Antonio; Latorre, Claudio; Gutierrez, Rodrigo A.
通讯作者	Gutierrez, RA
来源期刊	ECOGRAPHY
ISSN	0906-7590
EISSN	1600-0587
英文摘要	Estimating total plant diversity in extreme or hyperarid environments can be challenging, as adaptations to pronounced climate variability include evading prolonged stress periods through seeds or specialized underground organs. Short-term surveys of these ecosystems are thus likely poor estimators of actual diversity. Here we develop a multimethod strategy to obtain a more complete understanding of plant diversity from a community in the Atacama Desert. We explicitly test environmental DNA-based techniques (eDNA) to see if they can reveal the observed and 'hidden' (dormant or locally rare) species. To estimate total plant diversity, we performed long-term traditional surveys during eight consecutive years, including El Nino and La Nina events, we then analyzed eDNA from soil samples using high-throughput sequencing. We further used soil pollen analysis and soil seed bank germination assays to identify 'hidden' species. Each approach offers different subsets of current biodiversity at different taxonomic, spatial and temporal resolution, with a total of 92 taxa identified along the transect. Traditional field surveys identified 77 plant species over eight consecutive years. Observed community composition greatly varies interannually, with only 22 species seen every year. eDNA analysis revealed 37 taxa, eight of which were 'hidden' in our field surveys. Soil samples contain a viable seed bank of 21 taxa. Soil pollen (27 taxa) and eDNA analysis show affinities with vegetation at the landscape scale but a weak relationship to local plot diversity. Multimethod approaches (including eDNA) in deserts are valuable tools that add to a comprehensive assessment of biodiversity in such extreme environments, where using a single method or observations over a few years is insufficient. Our results can also explain the resilience of Atacama plant communities as 'hidden' taxa may have been active in the recent past or could even emerge in the future as accelerated global environmental change continues unabated.
英文关键词	Atacama eDNA environmental metabarcoding hidden diversity plant community soils
类型	Article ; Early Access
语种	英语
开放获取类型	DOAJ Gold
收录类别	SCI-E
WOS记录号	WOS:000576586200001
WOS关键词	MODERN POLLEN RAIN ; SOIL SEED BANK ; ATACAMA DESERT ; NORTHERN CHILE ; EXTRACELLULAR DNA ; RODENT MIDDENS ; CENTRAL ANDES ; CLIMATE ; VEGETATION ; BIODIVERSITY
WOS类目	Biodiversity Conservation ; Ecology
WOS研究方向	Biodiversity & Conservation ; Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/328332
作者单位	[Carrasco-Puga, Gabriela; Diaz, Francisca P.; Soto, Daniela C.; Hernandez-Castro, Catalina; Contreras-Lopez, Orlando; Gutierrez, Rodrigo A.] Pontificia Univ Catolica Chile, Dept Genet Mol & Microbiol, Santiago, Chile; [Carrasco-Puga, Gabriela; Diaz, Francisca P.; Soto, Daniela C.; Hernandez-Castro, Catalina; Contreras-Lopez, Orlando; Gutierrez, Rodrigo A.] FONDAP Ctr Genome Regulat & ANID, Millennium Sci Initiat Program, Millennium Inst Integrat Biol iBio, Santiago, Chile; [Maldonado, Antonio] Ctr Estudios Avanzados Zonas Aridas, La Serena, Chile; [Maldonado, Antonio] Univ La Serena, Inst Invest Multidisciplinario Ciencia & Tecnol, La Serena, Chile; [Maldonado, Antonio] Univ Catolica Norte, Dept Biol Marina, Coquimbo, Chile; [Latorre, Claudio] Pontificia Univ Catolica Chile, Ctr UC Desierto Atacama, Dept Ecol, Santiago, Chile; [Latorre, Claudio] Inst Ecol & Biodivers IEB, Santiago, Chile
推荐引用方式 GB/T 7714	Carrasco-Puga, Gabriela,Diaz, Francisca P.,Soto, Daniela C.,et al. Revealing hidden plant diversity in arid environments[J].
APA	Carrasco-Puga, Gabriela.,Diaz, Francisca P..,Soto, Daniela C..,Hernandez-Castro, Catalina.,Contreras-Lopez, Orlando.,...&Gutierrez, Rodrigo A..
MLA	Carrasco-Puga, Gabriela,et al."Revealing hidden plant diversity in arid environments".ECOGRAPHY