干旱区生态环境知识资源中心(Arid): Identification of the endosymbionts fromSulla spinosissimagrowing in a lead mine tailings in Eastern Morocco asMesorhizobium camelthornisv. aridi

Arid

DOI	10.1111/jam.14834
	Identification of the endosymbionts fromSulla spinosissimagrowing in a lead mine tailings in Eastern Morocco asMesorhizobium camelthornisv. aridi
	Lamin, H.; Alami, S.; Bouhnik, O.; Bennis, M.; Benkritly, S.; Abdelmoumen, H.; Bedmar, E. J.; Missbah-El Idrissi, M.
通讯作者	Idrissi, MME
来源期刊	JOURNAL OF APPLIED MICROBIOLOGY
ISSN	1364-5072
EISSN	1365-2672
英文摘要	Aims To identify the bacteria nodulatingSulla spinosissimagrowing profusely in a lead and zinc mine tailings in Eastern Morocco. Methods and Results In all, 32 rhizobial cultures, isolated from root nodules ofS. spinosissimagrowing in soils of the mining site, were tolerant to different heavy metals. The ERIC-polymerase chain reaction (PCR) fingerprinting analysis clustered the isolates into seven different groups, and the analysis of the 16S rRNA sequences of four selected representative strains, showed they were related to different species of the genusMesorhizobium. TheatpD,glnIIandrecAhousekeeping genes analysis confirmed the affiliation of the four representative strains toMesorhizobium camelthorniCCNWXJ40-4(T), with similarity percentages varying from 96 center dot 30 to 98 center dot 30%. The sequences of thenifHgene had 97 center dot 33-97 center dot 78% similarities with that ofM. camelthorniCCNWXJ40-4(T); however, thenodCphylogeny of the four strains diverged from the type and other reference strains ofM. camelthorniand formed a separated cluster. The four strains nodulate alsoAstragalus gombiformisandA. armatusbut did not nodulateA. boeticus,Vachellia gummifera,Prosopis chilensis,Cicer arietinum, Lens culinaris,Medicago truncatula, Lupinus luteusorPhaseolus vulgaris. Conclusions Based on similarities of thenodCsymbiotic gene and differences in the host range, the strains isolated fromS. spinosissimagrowing in soils of the Sidi Boubker mining site may form a different symbiovar withinMesorhizobiumfor which the name aridi is proposed. Significance and Impact of the Study In this work, we show that strains ofM. camelthornispecies nodulatingS. spinosissimain the arid area of Eastern Morocco constitute a distinct phylogenetic clade of nodulation genes; we named symbiovar aridi, which encompasses also mesorhizobia from other Mediterranean desert legumes.
英文关键词	16S rRNA genes biodiversity housekeeping genes Mesorhizobium camelthorni mining site tailings nodulation genes Sulla spinosissima Symbiovar aridi
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000567894600001
WOS关键词	MULTILOCUS SEQUENCE-ANALYSIS ; HEDYSARUM ; RHIZOBIA ; DIVERSITY ; SYMBIOSIS ; GENETICS ; ENSIFER ; LEGUMES ; NODULES ; METALS
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/328222
作者单位	[Lamin, H.; Alami, S.; Bouhnik, O.; Bennis, M.; Abdelmoumen, H.; Missbah-El Idrissi, M.] Mohammed V Univ Rabat, Fac Sci, Ctr Biotechnol Vegetales & Microbiennes Biodivers, Rabat, Morocco; [Benkritly, S.] Univ Oran1 Ahmed Ben Bella, Fac Sci, Dept Biotechnol, Lab Biotechnol Rhizobiums & Ameliorat Plantes LBR, Es Senia, Algeria; [Bedmar, E. J.] CSIC, Estn Expt Zaidin, Dept Soil Microbiol & Symbiot Syst, Granada, Spain
推荐引用方式 GB/T 7714	Lamin, H.,Alami, S.,Bouhnik, O.,et al. Identification of the endosymbionts fromSulla spinosissimagrowing in a lead mine tailings in Eastern Morocco asMesorhizobium camelthornisv. aridi[J].
APA	Lamin, H..,Alami, S..,Bouhnik, O..,Bennis, M..,Benkritly, S..,...&Missbah-El Idrissi, M..
MLA	Lamin, H.,et al."Identification of the endosymbionts fromSulla spinosissimagrowing in a lead mine tailings in Eastern Morocco asMesorhizobium camelthornisv. aridi".JOURNAL OF APPLIED MICROBIOLOGY