干旱区生态环境知识资源中心(Arid): Expression patterns and functional analysis of the short neuropeptide F and NPF receptor genes inRhopalosiphum padi

Arid

DOI	10.1111/1744-7917.12842
	Expression patterns and functional analysis of the short neuropeptide F and NPF receptor genes inRhopalosiphum padi
	Peng, Xiong; Chen, Cheng; Huang, Yixiao; Wang, Suji; Su, Sha; Chen, Maohua
通讯作者	Chen, MH
来源期刊	INSECT SCIENCE
ISSN	1672-9609
EISSN	1744-7917
英文摘要	The short neuropeptide F (sNPF) and NPF receptor (NPFR) genes play important roles in many physiological processes. However, information on the survival-related functions ofsNPFandNPFRunder different stress conditions is lacking in aphids. In this study, we clonedsNPFandNPFR, and investigated the expression levels of these genes in different developmental stages, wing morphs, and stress conditions of the bird cherry-oat aphid (Rhopalosiphum padiL.), an important agricultural pest. ThesNPFandNPFRtranscript levels varied among developmental stages, and their expression levels in alate females were significantly higher than those in apterous females. In addition, starvation resulted in significantly increased sNPFexpression, which then recovered after refeeding. Heat stress and insecticides significantly affected transcription of both genes.sNPFandNPFRknockdown inR. padiusing RNA interference revealed optimal interference efficiency at 48 h post-injection.sNPFknockdown significantly decreased adult longevity, 15-d fecundity, and food intake. Additionally, mortality under starvation, insecticides, and heat stress conditions was significantly higher after injection with double-strandedsNPFinR. padi.NPFRknockdown significantly affected food intake and starvation resistance inR. padi. These results strongly indicate thatsNPFplays vital roles in food intake, longevity, and reproduction inR. padi, and it can significantly affect the pest's response to stress conditions.
英文关键词	fecundity food intake heat stress insecticides starvation survival rate
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000550433900001
WOS关键词	RHOPALOSIPHUM-PADI ; MOLECULAR-CLONING ; FEEDING-BEHAVIOR ; DESERT LOCUST ; SNPF
WOS类目	Entomology
WOS研究方向	Entomology
来源机构	西北农林科技大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/328104
作者单位	[Peng, Xiong; Chen, Cheng; Huang, Yixiao; Wang, Suji; Su, Sha; Chen, Maohua] Northwest A&F Univ, Minist Agr & Rural Affairs, Key Lab Crop Pest Integrated Pest Management Loes, State Key Lab Crop Stress Biol Arid Areas, Yangling, Shaanxi, Peoples R China
推荐引用方式 GB/T 7714	Peng, Xiong,Chen, Cheng,Huang, Yixiao,et al. Expression patterns and functional analysis of the short neuropeptide F and NPF receptor genes inRhopalosiphum padi[J]. 西北农林科技大学.
APA	Peng, Xiong,Chen, Cheng,Huang, Yixiao,Wang, Suji,Su, Sha,&Chen, Maohua.
MLA	Peng, Xiong,et al."Expression patterns and functional analysis of the short neuropeptide F and NPF receptor genes inRhopalosiphum padi".INSECT SCIENCE