干旱区生态环境知识资源中心(Arid): Genome-wide association study uncovers genomic regions associated with grain iron, zinc and protein content in pearl millet

Arid

DOI	10.1038/s41598-020-76230-y
	Genome-wide association study uncovers genomic regions associated with grain iron, zinc and protein content in pearl millet
	Pujar, Mahesh; Gangaprasad, S.; Govindaraj, Mahalingam; Gangurde, Sunil S.; Kanatti, A.; Kudapa, Himabindu
通讯作者	Govindaraj, M
来源期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
出版年	2020
卷号	10 期号:1
英文摘要	Pearl millet hybrids biofortified with iron (Fe) and zinc (Zn) promise to be part of a long-term strategy to combat micronutrient malnutrition in the arid and semi-arid tropical (SAT) regions of the world. Biofortification through molecular breeding is the way forward to achieving a rapid trait-based breeding strategy. This genome-wide association study (GWAS) was conducted to identify significant marker-trait associations (MTAs) for Fe, Zn, and protein content (PC) for enhanced biofortification breeding. A diverse panel of 281 advanced inbred lines was evaluated for Fe, Zn, and PC over two seasons. Phenotypic evaluation revealed high variability (Fe: 32-120 mg kg(-1), Zn: 19-87 mg kg(-1), PC: 8-16%), heritability (h(bs)(2)>= 90%) and significantly positive correlation among Fe, Zn and PC (P=0.01), implying concurrent improvement. Based on the Diversity Arrays Technology (DArT) seq assay, 58,719 highly informative SNPs were filtered for association mapping. Population structure analysis showed six major genetic groups (K=6). A total of 78 MTAs were identified, of which 18 were associated with Fe, 43 with Zn, and 17 with PC. Four SNPs viz., Pgl04_64673688, Pgl05_135500493, Pgl05_144482656, and Pgl07_101483782 located on chromosomes Pgl04 (1), Pgl05 (2) and Pgl07 (1), respectively were co-segregated for Fe and Zn. Promising genes, 'Late embryogenesis abundant protein', 'Myb domain', 'pentatricopeptide repeat', and 'iron ion binding' coded by 8 SNPs were identified. The SNPs/genes identified in the present study presents prospects for genomics assisted biofortification breeding in pearl millet.
类型	Article
语种	英语
开放获取类型	Green Accepted, gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000595151600027
WOS关键词	LINKAGE DISEQUILIBRIUM ; PLANTS ; DIVERSITY ; MARKERS ; GENES ; LOCI
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
来源机构	International Crops Research Institute for the Semi-Arid Tropics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/327816
作者单位	[Pujar, Mahesh; Govindaraj, Mahalingam; Gangurde, Sunil S.; Kanatti, A.; Kudapa, Himabindu] Int Crops Res Inst Semi Arid Trop, Patancheru 502324, Telangana, India; [Pujar, Mahesh; Gangaprasad, S.] Univ Agr Sci, Shivamogga 577225, Karnataka, India
推荐引用方式 GB/T 7714	Pujar, Mahesh,Gangaprasad, S.,Govindaraj, Mahalingam,et al. Genome-wide association study uncovers genomic regions associated with grain iron, zinc and protein content in pearl millet[J]. International Crops Research Institute for the Semi-Arid Tropics,2020,10(1).
APA	Pujar, Mahesh,Gangaprasad, S.,Govindaraj, Mahalingam,Gangurde, Sunil S.,Kanatti, A.,&Kudapa, Himabindu.(2020).Genome-wide association study uncovers genomic regions associated with grain iron, zinc and protein content in pearl millet.SCIENTIFIC REPORTS,10(1).
MLA	Pujar, Mahesh,et al."Genome-wide association study uncovers genomic regions associated with grain iron, zinc and protein content in pearl millet".SCIENTIFIC REPORTS 10.1(2020).