干旱区生态环境知识资源中心(Arid): Three neuroparsin genes from oriental river prawn, Macrobrachium nipponense, involved in ovary maturation

Arid

DOI	10.1007/s13205-020-02531-8
	Three neuroparsin genes from oriental river prawn, Macrobrachium nipponense, involved in ovary maturation
	Qiao, Hui; Xiong, Yiwei; Jiang, Sufei; Zhang, Wenyi; Xu, Lei; Jin, Shubo; Gong, Yongsheng; Wu, Yan; Fu, Hongtuo
通讯作者	Fu, HT
来源期刊	3 BIOTECH
ISSN	2190-572X
EISSN	2190-5738
出版年	2020
卷号	10 期号:12
英文摘要	In this study, we identified three neuroparsin (NP) genes in Macrobrachium nipponense: Mn-NP1, Mn-NP2, and Mn-NP3, encoding 99, 100, and 101 amino acid proteins, respectively. Multiple sequence alignments showed that these genes contained 12 cysteine residues, of which 11 were at conserved positions. The total sequence identity between the genes was 47.5%, and they showed a high degree of sequence identity (> 54% similarity) with other crustacean genes. Phylogenetic tree analysis showed that Mn-NPs were clustered at different branches, indicating that Mn-NPs may have different functions. Tissue distribution data revealed that the three genes were present in males and females during the breeding and nonbreeding season, but their expression patterns differed. Mn-NP1 was highly expressed in the breeding season, in the male testis, and highly expressed in the nonbreeding season, in the female ovary. Mn-NP3 exhibited biased female expression in the breeding and nonbreeding season, with dominant expression in the ovary. All Mn-NPs were detected during embryo development, but with different expression patterns. These data indicated that Mn-NP1 may function during embryonic development, and that Mn-NP2 may be expressed during early embryo cell division, and late larval development. Mn-NP3 expression patterns reflected maternal inheritance, and may be associated with ovarian maturation. These expression data suggested that Mn-NP1 and Mn-NP2 are negatively correlated with ovarian development, with inhibition roles during this development. Mn-NP3 may be involved in vitellogenesis.
英文关键词	Macrobrachium nipponense Neuroparsin Ovary maturation Reproduction
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000594641000001
WOS关键词	TRANSCRIPT DISTRIBUTION ; DESERT LOCUST ; CDNA CLONING ; MOLECULAR-CLONING ; EXPRESSION ; FAMILY ; PCR ; HORMONE ; MEMBERS
WOS类目	Biotechnology & Applied Microbiology
WOS研究方向	Biotechnology & Applied Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/327789
作者单位	[Qiao, Hui; Xiong, Yiwei; Jiang, Sufei; Zhang, Wenyi; Jin, Shubo; Gong, Yongsheng; Wu, Yan; Fu, Hongtuo] Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Minist Agr, Key Lab Freshwater Fisheries & Germplasm Resource, Wuxi, Jiangsu, Peoples R China; [Fu, Hongtuo] Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Qiao, Hui,Xiong, Yiwei,Jiang, Sufei,et al. Three neuroparsin genes from oriental river prawn, Macrobrachium nipponense, involved in ovary maturation[J],2020,10(12).
APA	Qiao, Hui.,Xiong, Yiwei.,Jiang, Sufei.,Zhang, Wenyi.,Xu, Lei.,...&Fu, Hongtuo.(2020).Three neuroparsin genes from oriental river prawn, Macrobrachium nipponense, involved in ovary maturation.3 BIOTECH,10(12).
MLA	Qiao, Hui,et al."Three neuroparsin genes from oriental river prawn, Macrobrachium nipponense, involved in ovary maturation".3 BIOTECH 10.12(2020).