干旱区生态环境知识资源中心(Arid): Gene Sequences of Potential Targets of Insecticidal PF2 Lectin Identified from the Larval De Novo Transcriptome of the Mexican Bean Weevil (Zabrotes Subfasciatus; Boheman 1833)

Arid

DOI	10.3390/insects11110736
	Gene Sequences of Potential Targets of Insecticidal PF2 Lectin Identified from the Larval De Novo Transcriptome of the Mexican Bean Weevil (Zabrotes Subfasciatus; Boheman 1833)
	Lagarda-Diaz, Irlanda; Hernandez-Onate, Miguel Angel; Huerta-Ocampo, Jose Angel; Guzman-Partida, Ana M.; Winzerling, Joy; Geiser, Dawn; Vazquez-Moreno, Luz
通讯作者	Vazquez-Moreno, L
来源期刊	INSECTS
EISSN	2075-4450
出版年	2020
卷号	11 期号:11
英文摘要	Simple Summary The Mexican bean weevil Zabrotes subfasciatus is a major insect pest of stored beans. We have previously reported that the PF2 lectin, which is a protein found in the desert wild legume Olneya tesota (Palo Fierro), is toxic to Z. subfasciatus by inhibiting its early larval development. The use of proteomic means allowed us to identify PF2 targets in the midgut of Z. subfasciatus larvae. However, efforts to completely elucidate the insecticidal mechanism of PF2, as well as novel potential targets for insecticidal compounds, have been hindered by the lack of available genomic and proteomic information of non-model insects. Therefore, in this work we massively sequenced and analyzed the transcripts expressed in the larval stage of Z. subfasciatus, which is the first transcriptome reported for this insect. A total of 29,029 transcript sequences were identified, of which 30 sequences encode putative targets of PF2. The functional characteristics and biochemical, biological, or molecular roles for 15,124 sequences were established by means of bioinformatics tools. This study significantly increased the available genetic resources for Zabrotes and related insect species and will be helpful for any kind of future study that requires information on genes or protein sequences. The available genomic and proteomic information of non-model organisms is often underrepresented in public databases hindering their study at molecular, cellular, and physiological levels. Information on Zabrotes subfasciatus (Mexican bean weevil) is poorly represented in databases, yet it is a major pest of common beans. We report the transcriptome of Z. subfasciatus larvae; transcripts were sequenced using an Illumina RNA-Seq technology and assembled de novo identifying 29,029 unigenes with an average size of 1168 bp and an N50 value of 2196 bp. About 15,124 unigenes (52%) were functionally annotated and categorized. Further analysis revealed 30 unigene sequences encoding putative targets of the insecticidal PF2 lectin. The complete deduced amino acid sequences of eight selected proteins potentially related to insecticidal mechanism of Palo Fierro 2 (PF2) were used for predicting probable N-glycosylation sites and analyzing phylogenetic relationships with insect sequences. This work provides a dramatic increase in the genetic resources available for Coleopterans and set the basis for developing future studies on biological aspects and potential control strategies for Z. subfasciatus.
英文关键词	coleopteran insect pest insect RNA-seq Transcriptomics
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000594013800001
WOS关键词	MITOCHONDRIAL PROCESSING PEPTIDASE ; FLIGHT-MUSCLE MITOCHONDRIA ; COLEOPTERA BRUCHIDAE ; OLNEYA-TESOTA ; PROTEINS ; ANNOTATION ; PROHIBITIN ; SUBUNIT ; MIDGUT ; MODE
WOS类目	Entomology
WOS研究方向	Entomology
来源机构	University of Arizona
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/327749
作者单位	[Lagarda-Diaz, Irlanda] Univ Sonora, CONACYT, Hermosillo 83000, Sonora, Mexico; [Hernandez-Onate, Miguel Angel; Huerta-Ocampo, Jose Angel] Ctr Invest Alimentac & Desarrollo AC, CONACYT, Hermosillo 83304, Sonora, Mexico; [Guzman-Partida, Ana M.; Vazquez-Moreno, Luz] eCtr Invest Alimentac & Desarrollo AC, Hermosillo 83304, Sonora, Mexico; [Winzerling, Joy; Geiser, Dawn] Univ Arizona, Div Agr Life & Vet Sci, Dept Nutr Sci, Tucson, AZ 85721 USA
推荐引用方式 GB/T 7714	Lagarda-Diaz, Irlanda,Hernandez-Onate, Miguel Angel,Huerta-Ocampo, Jose Angel,et al. Gene Sequences of Potential Targets of Insecticidal PF2 Lectin Identified from the Larval De Novo Transcriptome of the Mexican Bean Weevil (Zabrotes Subfasciatus; Boheman 1833)[J]. University of Arizona,2020,11(11).
APA	Lagarda-Diaz, Irlanda.,Hernandez-Onate, Miguel Angel.,Huerta-Ocampo, Jose Angel.,Guzman-Partida, Ana M..,Winzerling, Joy.,...&Vazquez-Moreno, Luz.(2020).Gene Sequences of Potential Targets of Insecticidal PF2 Lectin Identified from the Larval De Novo Transcriptome of the Mexican Bean Weevil (Zabrotes Subfasciatus; Boheman 1833).INSECTS,11(11).
MLA	Lagarda-Diaz, Irlanda,et al."Gene Sequences of Potential Targets of Insecticidal PF2 Lectin Identified from the Larval De Novo Transcriptome of the Mexican Bean Weevil (Zabrotes Subfasciatus; Boheman 1833)".INSECTS 11.11(2020).