干旱区生态环境知识资源中心(Arid): Toxicological Characterization and Phospholipase D Activity of the Venom of the Spider Sicarius thomisoides

Arid

DOI	10.3390/toxins12110702
	Toxicological Characterization and Phospholipase D Activity of the Venom of the Spider Sicarius thomisoides
	Aran-Sekul, Tomas; Percic-Sarmiento, Ivanka; Valencia, Veronica; Olivero, Nelly; Rojas, Jose M.; Araya, Jorge E.; Taucare-Rios, Andres; Catalan, Alejandro
通讯作者	Catalan, A
来源期刊	TOXINS
EISSN	2072-6651
出版年	2020
卷号	12 期号:11
英文摘要	Envenomation by Loxosceles spiders (Sicariidae family) has been thoroughly documented. However, little is known about the potential toxicity of members from the Sicarius genus. Only the venom of the Brazilian Sicarius ornatus spider has been toxicologically characterized. In Chile, the Sicarius thomisoides species is widely distributed in desert and semidesert environments, and it is not considered a dangerous spider for humans. This study aimed to characterize the potential toxicity of the Chilean S. thomisoides spider. To do so, specimens of S. thomisoides were captured in the Atacama Desert, the venom was extracted, and the protein concentration was determined. Additionally, the venoms were analyzed by electrophoresis and Western blotting using anti-recombinant L. laeta PLD1 serum. Phospholipase D enzymatic activity was assessed, and the hemolytic and cytotoxic effects were evaluated and compared with those of the L. laeta venom. The S. thomisoides venom was able to hydrolyze sphingomyelin as well as induce complement-dependent hemolysis and the loss of viability of skin fibroblasts with a dermonecrotic effect of the venom in rabbits. The venom of S. thomisoides showed intraspecific variations, with a similar protein pattern as that of L. laeta venom at 32-35 kDa, recognized by serum anti-LlPLD1. In this context, we can conclude that the venom of Sicarius thomisoides is similar to Loxosceles laeta in many aspects, and the dermonecrotic toxin present in their venom could cause severe harm to humans; thus, precautions are necessary to avoid exposure to their bite.
英文关键词	Sicarius thomisoides phospholipase D venom toxicity
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000593899300001
WOS关键词	SPHINGOMYELINASE D ACTIVITY ; INTERMEDIA BROWN SPIDER ; LOXOSCELES-INTERMEDIA ; CLOSTRIDIUM-PERFRINGENS ; IDENTIFICATION ; KERATINOCYTES ; ISOFORMS ; FAMILY ; LAETA ; FANGS
WOS类目	Food Science & Technology ; Toxicology
WOS研究方向	Food Science & Technology ; Toxicology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/327733
作者单位	[Aran-Sekul, Tomas; Percic-Sarmiento, Ivanka; Valencia, Veronica; Olivero, Nelly; Rojas, Jose M.; Araya, Jorge E.; Catalan, Alejandro] Univ Antofagasta, Fac Ciencias Salud, Dept Tecnol Med, Lab Parasitol Mol, Antofagasta 1270300, Chile; [Taucare-Rios, Andres] Univ Arturo Prat, Fac Ciencias, Iquique 1110939, Chile; [Taucare-Rios, Andres] Univ Arturo Prat, Ctr Invest Medio Ambiente CENIMA, Iquique 1110939, Chile
推荐引用方式 GB/T 7714	Aran-Sekul, Tomas,Percic-Sarmiento, Ivanka,Valencia, Veronica,et al. Toxicological Characterization and Phospholipase D Activity of the Venom of the Spider Sicarius thomisoides[J],2020,12(11).
APA	Aran-Sekul, Tomas.,Percic-Sarmiento, Ivanka.,Valencia, Veronica.,Olivero, Nelly.,Rojas, Jose M..,...&Catalan, Alejandro.(2020).Toxicological Characterization and Phospholipase D Activity of the Venom of the Spider Sicarius thomisoides.TOXINS,12(11).
MLA	Aran-Sekul, Tomas,et al."Toxicological Characterization and Phospholipase D Activity of the Venom of the Spider Sicarius thomisoides".TOXINS 12.11(2020).