干旱区生态环境知识资源中心(Arid): Evaluation of RNA Interference for Control of the Grape Mealybug Pseudococcus maritimus (Hemiptera: Pseudococcidae)

Arid

DOI	10.3390/insects11110739
	Evaluation of RNA Interference for Control of the Grape Mealybug Pseudococcus maritimus (Hemiptera: Pseudococcidae)
	Arora, Arinder K.; Clark, Noah; Wentworth, Karen S.; Hesler, Stephen; Fuchs, Marc; Loeb, Greg; Douglas, Angela E.
通讯作者	Douglas, AE
来源期刊	INSECTS
EISSN	2075-4450
出版年	2020
卷号	11 期号:11
英文摘要	Simple Summary: RNA interference (RNAi) is a defense mechanism that protects insects from viruses by targeting and degrading RNA. This feature has been exploited to reduce the expression of endogenous RNA for determining functions of various genes and for killing insect pests by targeting genes that are vital for insect survival. When dsRNA matching perfectly to the target RNA is administered, the RNAi machinery dices the dsRNA into similar to 21 bp fragments (known as siRNAs) and one strand of siRNA is employed by the RNAi machinery to target and degrade the target RNA. In this study we used a cocktail of dsRNAs targeting grape mealybug's aquaporin and sucrase genes to kill the insect. Aquaporins and sucrases are important genes enabling these insects to maintain water relations indispensable for survival and digest complex sugars in the diet of plant sap-feeding insects, including mealybugs. In our experiments, administration of dsRNA caused a reduction in expression of the target genes and an increase in insect mortality. These results provide support for the application of RNAi to control the grape mealybug. The grape mealybug Pseudococcus maritimus (Ehrhorn, 1900) (Hemiptera: Pseudococcidae) is a significant pest of grapevines (Vitis spp.) and a vector of disease-causing grape viruses, linked to its feeding on phloem sap. The management of this pest is constrained by the lack of naturally occurring resistance traits in Vitis. Here, we obtained proof of concept that RNA interference (RNAi) using double-stranded RNA (dsRNA) molecules against essential genes for phloem sap feeding can depress insect survival. The genes of interest code for an aquaporin (AQP) and a sucrase (SUC) that are required for osmoregulation in related phloem sap-feeding hemipteran insects (aphids and whiteflies). In parallel, we investigated the grape mealybug genes coding non-specific nucleases (NUC), which reduce RNAi efficacy by degrading administered dsRNA. Homologs of AQP and SUC with experimentally validated function in aphids, together with NUC, were identified in the published transcriptome of the citrus mealybug Planococcus citri by phylogenetic analysis, and sequences of the candidate genes were obtained for Ps. maritimus by PCR with degenerate primers. Using this first sequence information for Ps. maritimus, dsRNA was prepared and administered to the insects via an artificial diet. The treatment comprising dsRNA against AQP, SUC and NUC significantly increased insect mortality over three days, relative to dsRNA-free controls. The dsRNA constructs for AQP and NUC were predicted, from sequence analysis to have some activity against other mealybugs, but none of the three dsRNA constructs have predicted activity against aphids. This study provides the basis to develop in planta RNAi strategies against Ps. maritimus and other mealybug pests of grapevines.
英文关键词	grapevine pests mealybugs osmoregulation genes phloem-feeding insects Pseudococcus maritimus RNA interference
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000593878000001
WOS关键词	DOUBLE-STRANDED-RNA ; PEA APHID ; FUNCTIONAL-CHARACTERIZATION ; DSRNA DEGRADATION ; NUCLEASE ACTIVITY ; DESERT LOCUST ; GUT SUCRASE ; INSECT ; OSMOREGULATION ; EXPRESSION
WOS类目	Entomology
WOS研究方向	Entomology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/327731
作者单位	[Arora, Arinder K.; Clark, Noah; Douglas, Angela E.] Cornell Univ, Dept Entomol, Ithaca, NY 14850 USA; [Wentworth, Karen S.; Hesler, Stephen; Loeb, Greg] Cornell Univ, Dept Entomol, Geneva, NY 14456 USA; [Fuchs, Marc] Cornell Univ, Sch Integrat Plant Sci, Geneva, NY 14456 USA; [Douglas, Angela E.] Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA
推荐引用方式 GB/T 7714	Arora, Arinder K.,Clark, Noah,Wentworth, Karen S.,et al. Evaluation of RNA Interference for Control of the Grape Mealybug Pseudococcus maritimus (Hemiptera: Pseudococcidae)[J],2020,11(11).
APA	Arora, Arinder K..,Clark, Noah.,Wentworth, Karen S..,Hesler, Stephen.,Fuchs, Marc.,...&Douglas, Angela E..(2020).Evaluation of RNA Interference for Control of the Grape Mealybug Pseudococcus maritimus (Hemiptera: Pseudococcidae).INSECTS,11(11).
MLA	Arora, Arinder K.,et al."Evaluation of RNA Interference for Control of the Grape Mealybug Pseudococcus maritimus (Hemiptera: Pseudococcidae)".INSECTS 11.11(2020).