干旱区生态环境知识资源中心(Arid): Motilibacter deserti sp. nov. and Motilibacter aurantiacus sp. nov., two novel actinobacteria isolated from soil of Cholistan Desert and emended description of the genus Motilibacter

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DOI	10.1016/j.syapm.2020.126150
	Motilibacter deserti sp. nov. and Motilibacter aurantiacus sp. nov., two novel actinobacteria isolated from soil of Cholistan Desert and emended description of the genus Motilibacter
	Liu, Shao-Wei; Ye, Jing-Jing; Lu, Qin-Pei; Cheema, Mohsin Tassawar; Abbas, Muhammad; Huang, Da-Lin; Sajid, Imran; Sun, Cheng-Hang
通讯作者	Sajid, I
来源期刊	SYSTEMATIC AND APPLIED MICROBIOLOGY
ISSN	0723-2020
EISSN	1618-0984
出版年	2020
卷号	43 期号:6
英文摘要	Two novel actinobacterial strains, designated as E257(T) and K478(T), were isolated from hyper-arid soil samples collected in Cholistan Desert, Pakistan. Comparative analysis of 16S rRNA genes showed that strains E257(T) and K478(T) were assigned to the genus Motilibacter, being their closest relative M. rhizosphaerae RS-16(T) with 97.3% and 96.7% similarities, respectively. The sequence similarity between strain E257(T) and K478(T) was 98.9%. Phylogenetic analysis based on 16S rRNA gene sequences and phylogenomic analysis based on multiple genes of conserved core proteins exhibited that these two strains belonged to the genus Motilibacter and formed a robust cluster separated from the two type species of the genus Motilibacter. Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257(T) and K478(T) were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation. The two isolates were found to have chemotaxonomic, cultural and morphological properties consistent with their classification in the genus Motilibacter and also confirmed the differentiation from their closest species. The obtained results demonstrated that strains E257(T) and K478(T) represent two novel species of the genus Motilibacter, for which the names Motilibacter desertisp. nov. (type strain E257(T) = JCM 33651(T) = CGMCC 1.17159(T)) and Motilibacter aurantiacus sp. nov. (type strain K478(T) = JCM 33652(T) = CGMCC 1.17229(T)) are proposed. (C) 2020 Elsevier GmbH. All rights reserved.
英文关键词	Actinobacteria Motilibacter Genome comparison Phylogeny Taxonomy
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000593705400009
WOS关键词	ESCHERICHIA-COLI ; GLYCINE BETAINE ; HIGHER RANKS ; PROTEIN ; PROPOSAL ; CHOLINE ; SYSTEM ; MEMBER ; CLASSIFICATION ; TRANSCRIPTION
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/327719
作者单位	[Liu, Shao-Wei; Ye, Jing-Jing; Lu, Qin-Pei; Sun, Cheng-Hang] Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Biotechnol, Dept Microbial Chem, Beijing 100050, Peoples R China; [Liu, Shao-Wei; Sun, Cheng-Hang] Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Biotechnol, Beijing Key Lab Antimicrobial Agents, Beijing 100050, Peoples R China; [Ye, Jing-Jing; Huang, Da-Lin] Guilin Med Univ, Coll Basic Med Sci, Guilin 541004, Peoples R China; [Cheema, Mohsin Tassawar; Abbas, Muhammad; Sajid, Imran] Univ Punjab, Dept Microbiol & Mol Genet, Quid I Azam Campus, Lahore 54590, Pakistan
推荐引用方式 GB/T 7714	Liu, Shao-Wei,Ye, Jing-Jing,Lu, Qin-Pei,et al. Motilibacter deserti sp. nov. and Motilibacter aurantiacus sp. nov., two novel actinobacteria isolated from soil of Cholistan Desert and emended description of the genus Motilibacter[J],2020,43(6).
APA	Liu, Shao-Wei.,Ye, Jing-Jing.,Lu, Qin-Pei.,Cheema, Mohsin Tassawar.,Abbas, Muhammad.,...&Sun, Cheng-Hang.(2020).Motilibacter deserti sp. nov. and Motilibacter aurantiacus sp. nov., two novel actinobacteria isolated from soil of Cholistan Desert and emended description of the genus Motilibacter.SYSTEMATIC AND APPLIED MICROBIOLOGY,43(6).
MLA	Liu, Shao-Wei,et al."Motilibacter deserti sp. nov. and Motilibacter aurantiacus sp. nov., two novel actinobacteria isolated from soil of Cholistan Desert and emended description of the genus Motilibacter".SYSTEMATIC AND APPLIED MICROBIOLOGY 43.6(2020).