干旱区生态环境知识资源中心(Arid): Agrobacterium tumefaciens-Mediated Genetic Transformation of the Ect-endomycorrhizal Fungus Terfezia boudieri

Arid

DOI	10.3390/genes11111293
	Agrobacterium tumefaciens-Mediated Genetic Transformation of the Ect-endomycorrhizal Fungus Terfezia boudieri
	Satish, Lakkakula; Kamle, Madhu; Keren, Guy; Patil, Chandrashekhar D.; Yehezkel, Galit; Barak, Ze'ev; Kagan-Zur, Varda; Kushmaro, Ariel; Sitrit, Yaron
通讯作者	Sitrit, Y
来源期刊	GENES
EISSN	2073-4425
出版年	2020
卷号	11 期号:11
英文摘要	Mycorrhizal desert truffles such as Terfezia boudieri, Tirmania nivea, and Terfezia claveryi, form mycorrhizal associations with plants of the Cistaceae family. These valued truffles are still collected from the wild and not cultivated under intensive farming due to the lack of basic knowledge about their biology at all levels. Recently, several genomes of desert truffles have been decoded, enabling researchers to attempt genetic manipulations to enable cultivation. To execute such manipulations, the development of molecular tools for genes transformation into truffles is needed. We developed an Agrobacterium tumefaciens-mediated genetic transformation system in T. boudieri. This system was optimized for the developmental stage of the mycelia explants, bacterial optical density, infection and co-cultivation durations, and concentrations of the selection antibiotics. The pFPL-Rh plasmid harboring hph gene conferring hygromycin resistance as a selection marker and the red fluorescent protein gene were used as visual reporters. The optimal conditions were incubation with 200 mu M of acetosyringone, attaining a bacterial optical density of 0.3 OD600; transfer time of 45 min; and co-cultivation for 3 days. This is the first report on a transformation system for T. boudieri, and the proposed protocol can be adapted for the transformation of other important desert truffles as well as ectomycorrhizal species.
英文关键词	Agrobacterium tumefaciens desert truffles Ect-endomycorrhizal fungus genetic transformation hygromycin Terfezia boudieri
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000593373100001
WOS关键词	GREEN FLUORESCENT PROTEIN ; MYCORRHIZAL FUNGUS ; INSERTIONAL MUTAGENESIS ; ECTOMYCORRHIZAL FUNGI ; TUBER-BORCHII ; INTEGRATION ; TRUFFLES ; MARKER
WOS类目	Genetics & Heredity
WOS研究方向	Genetics & Heredity
来源机构	Ben-Gurion University of the Negev
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/327703
作者单位	[Satish, Lakkakula; Kamle, Madhu; Keren, Guy; Patil, Chandrashekhar D.; Yehezkel, Galit; Sitrit, Yaron] Ben Gurion Univ Negev, Jacob Blaustein Inst Desert Res, Albert Katz Int Sch Desert Studies, IL-84105 Beer Sheva, Israel; [Satish, Lakkakula; Kushmaro, Ariel] Ben Gurion Univ Negev, Avram & Stella Goldstein Goren Dept Biotechnol En, IL-84105 Beer Sheva, Israel; [Satish, Lakkakula; Kushmaro, Ariel] Ben Gurion Univ Negev, Ilse Katz Ctr Meso & Nanoscale Sci & Technol, IL-84105 Beer Sheva, Israel; [Barak, Ze'ev; Kagan-Zur, Varda] Ben Gurion Univ Negev, Dept Life Sci, IL-84105 Beer Sheva, Israel; [Kamle, Madhu] North Eastern Reg Inst Sci & Technol, Dept Forestry, Nirjuli 791109, Arunachal Prade, India; [Patil, Chandrashekhar D.] Univ Illinois, Dept Ophthalmol & Visual Sci, Chicago, IL 60612 USA
推荐引用方式 GB/T 7714	Satish, Lakkakula,Kamle, Madhu,Keren, Guy,et al. Agrobacterium tumefaciens-Mediated Genetic Transformation of the Ect-endomycorrhizal Fungus Terfezia boudieri[J]. Ben-Gurion University of the Negev,2020,11(11).
APA	Satish, Lakkakula.,Kamle, Madhu.,Keren, Guy.,Patil, Chandrashekhar D..,Yehezkel, Galit.,...&Sitrit, Yaron.(2020).Agrobacterium tumefaciens-Mediated Genetic Transformation of the Ect-endomycorrhizal Fungus Terfezia boudieri.GENES,11(11).
MLA	Satish, Lakkakula,et al."Agrobacterium tumefaciens-Mediated Genetic Transformation of the Ect-endomycorrhizal Fungus Terfezia boudieri".GENES 11.11(2020).