干旱区生态环境知识资源中心(Arid): UV-A Irradiation Increases Scytonemin Biosynthesis in Cyanobacteria Inhabiting Halites at Salar Grande, Atacama Desert

Arid

DOI	10.3390/microorganisms8111690
	UV-A Irradiation Increases Scytonemin Biosynthesis in Cyanobacteria Inhabiting Halites at Salar Grande, Atacama Desert
	Orellana, Gabriela; Gomez-Silva, Benito; Urrutia, Milton; Galetovic, Alexandra
通讯作者	Galetovic, A
来源期刊	MICROORGANISMS
EISSN	2076-2607
出版年	2020
卷号	8 期号:11
英文摘要	Microbial consortia inhabiting evaporitic salt nodules at the Atacama Desert are dominated by unculturable cyanobacteria from the genus Halothece. Halite nodules provide transparency to photosynthetically active radiation and diminish photochemically damaging UV light. Atacama cyanobacteria synthesize scytonemin, a heterocyclic dimer, lipid soluble, UV-filtering pigment (in vivo absorption maximum at 370 nm) that accumulates at the extracellular sheath. Our goal was to demonstrate if UV-A irradiations modulate scytonemin biosynthesis in ground halites containing uncultured Halothece sp. cyanobacteria. Pulverized halite nodules with endolithic colonization were incubated under continuous UV-A radiation (3.6 W/m(2)) for 96 h, at 67% relative humidity, mimicking their natural habitat. Scytonemin content and relative transcription levels of scyB gene (a key gene in the biosynthesis of scytonemin) were evaluated by spectrophotometry and quantitative RT-PCR, respectively. After 48 h under these experimental conditions, the ratio scytonemin/chlorophyll a and the transcription of scyB gene increased to a maximal 1.7-fold value. Therefore, endolithic Halothece cyanobacteria in halites are metabolically active and UV radiation is an environmental stressor with a positive influence on scyB gene transcription and scytonemin biosynthesis. Endolithobiontic cyanobacteria in Atacama show a resilient evolutive and adaptive strategy to survive in one of the most extreme environments on Earth.
英文关键词	scytonemin cyanobacteria UV-A radiation Halothece halite Atacama Desert
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000593162100001
WOS关键词	MICROBIAL COMMUNITIES ; HYPERARID CORE ; B RADIATION ; DESICCATION ; EVOLUTION ; PIGMENTS ; MOISTURE ; SEARCH ; CRUSTS ; ROCKS
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/327686
作者单位	[Orellana, Gabriela; Gomez-Silva, Benito; Galetovic, Alexandra] Univ Antofagasta, Fac Ciencias Salud, Lab Bioquim, Dept Biomed, Ave Angamos 601, Antofagasta 12701300, Chile; [Orellana, Gabriela; Gomez-Silva, Benito; Galetovic, Alexandra] Univ Antofagasta, Ctr Biotechnol & Bioengn CeBiB, Ave Angamos 601, Antofagasta 12701300, Chile; [Urrutia, Milton] Univ Antofagasta, Ave Angamos 601, Antofagasta 12701300, Chile
推荐引用方式 GB/T 7714	Orellana, Gabriela,Gomez-Silva, Benito,Urrutia, Milton,et al. UV-A Irradiation Increases Scytonemin Biosynthesis in Cyanobacteria Inhabiting Halites at Salar Grande, Atacama Desert[J],2020,8(11).
APA	Orellana, Gabriela,Gomez-Silva, Benito,Urrutia, Milton,&Galetovic, Alexandra.(2020).UV-A Irradiation Increases Scytonemin Biosynthesis in Cyanobacteria Inhabiting Halites at Salar Grande, Atacama Desert.MICROORGANISMS,8(11).
MLA	Orellana, Gabriela,et al."UV-A Irradiation Increases Scytonemin Biosynthesis in Cyanobacteria Inhabiting Halites at Salar Grande, Atacama Desert".MICROORGANISMS 8.11(2020).