干旱区生态环境知识资源中心(Arid): Double-stranded RNA in exosomes: Potential systemic RNA interference pathway in the Colorado potato beetle, Leptinotarsa decemlineata

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DOI	10.1016/j.aspen.2020.09.012
	Double-stranded RNA in exosomes: Potential systemic RNA interference pathway in the Colorado potato beetle, Leptinotarsa decemlineata
	Yoon, June-Sun; Kim, Kyungbo; Palli, Subba Reddy
通讯作者	Yoon, JS
来源期刊	JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
ISSN	1226-8615
EISSN	1876-7990
出版年	2020
卷号	23 期号:4 页码:1160-1164
英文摘要	Despite extensive research during the past decade elucidating the mechanism of RNA interference (RNAi) in insects, it is not clear how ingested or injected double-stranded RNA (dsRNA) triggers RNAi response in the whole body or even its progeny, which is referred to as systemic RNAi. In the present study, we aim to understand how the dsRNA delivered into cells causes systemic RNAi using Colorado potato beetle cells (Lepd-SL1). We first tested if dsRNA treatment induces systemic RNAi in Lepd-SL1 cells. Exposure of a new batch of Lepd-SL1 cells to the conditioned medium where Lepd-SL1 cells treated with dsRNA targeting inhibitor of apoptosis were grown for 6 h induced apoptosis in these new batch of cells. We hypothesized the exosomes in the conditioned medium are responsible for RNAi-inducing effect. To test this hypothesis, we isolated exosomes from the conditioned medium from Lepd-SL1 cells that had been treated with dsGFP (dsRNA targeting gene coding for green fluorescent protein) or dsLuc (dsRNA targeting gene coding for the luciferase) were grown. RNA present in the purified exosomes was analyzed to check if long dsRNA or siRNA is accumulated in them. The results from the electrophoretic mobility shift assay clearly showed that the long dsRNAs are present in the exosomes. By knockdown of candidate genes involved in endosome recycling and generation pathways, we found that Rab4 and Rab35 are involved in exosome production and transport.
英文关键词	Exosome RNA interference dsRNA Systemic RNAi Colorado potato beetle
类型	Article
语种	英语
开放获取类型	Bronze
收录类别	SCI-E
WOS记录号	WOS:000592595000041
WOS关键词	DESERT LOCUST ; DSRNA ; COLEOPTERA
WOS类目	Entomology
WOS研究方向	Entomology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/327658
作者单位	[Yoon, June-Sun; Kim, Kyungbo; Palli, Subba Reddy] Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA; [Yoon, June-Sun] Oregon State Univ, Dept Hort, Corvallis, OR 97330 USA
推荐引用方式 GB/T 7714	Yoon, June-Sun,Kim, Kyungbo,Palli, Subba Reddy. Double-stranded RNA in exosomes: Potential systemic RNA interference pathway in the Colorado potato beetle, Leptinotarsa decemlineata[J],2020,23(4):1160-1164.
APA	Yoon, June-Sun,Kim, Kyungbo,&Palli, Subba Reddy.(2020).Double-stranded RNA in exosomes: Potential systemic RNA interference pathway in the Colorado potato beetle, Leptinotarsa decemlineata.JOURNAL OF ASIA-PACIFIC ENTOMOLOGY,23(4),1160-1164.
MLA	Yoon, June-Sun,et al."Double-stranded RNA in exosomes: Potential systemic RNA interference pathway in the Colorado potato beetle, Leptinotarsa decemlineata".JOURNAL OF ASIA-PACIFIC ENTOMOLOGY 23.4(2020):1160-1164.