干旱区生态环境知识资源中心(Arid): Development of SSR loci in Prosopis tamarugo Phillipi and assessment of their transferability to species of the Strombocarpa section

Arid

DOI	10.5424/fs/2020292-16706
	Development of SSR loci in Prosopis tamarugo Phillipi and assessment of their transferability to species of the Strombocarpa section
	Contreras, Roberto; Carevic, Felipe S.; Porcile, Vincenzo; Arias, Mariana
通讯作者	Contreras, R
来源期刊	FOREST SYSTEMS
ISSN	2171-5068
EISSN	2171-9845
出版年	2020
卷号	29 期号:2
英文摘要	Aim of study: Phreatophyte species of the Prosopis genus are very important to natural ecosystems in Africa, South America and Asia due to their uses as food and seed sources and in agroforestry. In this research, through next-generation sequencing, we sought to search for and develop SSR markers in Prosopis tamarugo, in addition to assessing their transferability to other species in the Strombocarpa section. Area of study: The study was carried out in species of the Strombocarpa section collected in the Pampa del Tamarugal, located in the Atacama Desert (Chile); which is considered the driest and oldest desert on Earth. Material and methods: The next-generation sequencing for the development of simple sequence repeat (SSR) or microsatellite loci for genetic research in P. tamarugo and their transferability in Prosopis burkartii and Prosopis strombulifera was used. Main results: A total of similar to 90.000 microsatellite loci in P. tamarugo were found, and a set of 43 primer pairs was used for validating SSR locus amplification. We found a large difference in the percentage of amplified SSR markers between species of the Strombocarpa and Algarobia sections. Research highlights: The present study provides for the first time 24 polymorphic SSR markers for species in the Strombocarpa section, which could be a useful tool for estimating genetic structure, developing breeding programs, quantifying genetic diversity and performing population studies.
英文关键词	Strombocarpa section Prosopis tamarugo Atacama Desert microsatellites NGS
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000591689900014
WOS关键词	GENUS PROSOPIS ; GENETIC DIVERSITY ; MARKERS ; DESERT ; PHOTOSYNTHESIS ; HYBRIDIZATION ; POPULATIONS ; LEGUMINOSAE ; CHILENSIS ; DROUGHT
WOS类目	Forestry
WOS研究方向	Forestry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/327607
作者单位	[Contreras, Roberto; Porcile, Vincenzo; Arias, Mariana] Univ Atacama, Ctr Reg Invest & Desarrollo Sustentable Atacama C, Copayapu 485, Copiapo, Chile; [Carevic, Felipe S.] Univ Arturo Prat, Fac Recursos Nat Renovables, Lab Ecol Vegetal, Campus Huayquique, Iquique, Chile
推荐引用方式 GB/T 7714	Contreras, Roberto,Carevic, Felipe S.,Porcile, Vincenzo,et al. Development of SSR loci in Prosopis tamarugo Phillipi and assessment of their transferability to species of the Strombocarpa section[J],2020,29(2).
APA	Contreras, Roberto,Carevic, Felipe S.,Porcile, Vincenzo,&Arias, Mariana.(2020).Development of SSR loci in Prosopis tamarugo Phillipi and assessment of their transferability to species of the Strombocarpa section.FOREST SYSTEMS,29(2).
MLA	Contreras, Roberto,et al."Development of SSR loci in Prosopis tamarugo Phillipi and assessment of their transferability to species of the Strombocarpa section".FOREST SYSTEMS 29.2(2020).