干旱区生态环境知识资源中心(Arid): Molecular Evidence for the Association of Swarm Forming Desert Locust, Schistocerca gregaria gregaria (Forskal) in Pakistan with Highly Prevalent Subspecies in Sahara Desert of Africa

Arid

DOI	10.17582/journal.pjz/20200501020521
	Molecular Evidence for the Association of Swarm Forming Desert Locust, Schistocerca gregaria gregaria (Forskal) in Pakistan with Highly Prevalent Subspecies in Sahara Desert of Africa
	Ahmad, Jam Nazeer; Ahmad, Samina J. N.; Malik, Mubashir Ahmad; Ali, Abid; Ali, Muhammad; Ahmad, Ejaz; Tahir, Muhammad; Ashraf, Muhammad
通讯作者	Ahmad, JN ; Ahmad, SJN
来源期刊	PAKISTAN JOURNAL OF ZOOLOGY
ISSN	0030-9923
出版年	2020
卷号	52 期号:6 页码:2233-2242
英文摘要	Locust commonly known as short horned grasshopper is one of the most dangerous agricultural pests worldwide. There are various important species of large swarms forming of desert locusts found in different regions of the world. In early 2020, in Pakistan, huge swarm of desert locust infestation was observed in different provinces of Pakistan. Precise and correct identification of any pest is very important to start a proper control strategy against it. The current study was conducted to identify and characterize locust species and to determine their association with other swarm forming locust species worldwide. Morphological features and molecular characters were observed using digital camera and PCR technique, respectively. For molecular identification, DNA was extracted through CTAB method and polymerase chain reaction was performed using mitochondrial cytochrome oxidase I (COI) gene based primers. Gel electrophoresis of the PCR products of desert locusts indicated a 710bp amplified DNA fragment on 1.5% agarose gel. DNA sequence analysis of PCR product indicated that specimens shared a 99-100% identity with Schistocerca gregaria gregaria) (Forskal, 1775). Molecular phylogenetic analysis and evolutionary divergence study also revealed the formation of same cluster in phylogenetic tree with that of specific subspecies S. g. gregaria which further deviate genetically not only from closely related subspecies (S. g. flaviventris) but also other species reported from various countries. Migratory pattern of S. g. gregaria from Africa to Asia and neighboring countries with their potential routes also suggested developing sustainable policy to counter internal as well as external threat. This is the first report of molecular identification of S. g. grgaria and its association with most prevailed subspecies of Sahara desert in Northern Africa. Desert locust, S. g. gregaria is very destructive polyphagus pest therefore, immediately; control measures should be adopted to stop its further spread and infestation in Pakistan.
英文关键词	Desert locust Shistocerca gregaria gregaria PCR Molecular phylogeny DNA barcoding
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:000591649900024
WOS关键词	RHYNCHOPHORUS-FERRUGINEUS OLIVIER ; PHASE POLYMORPHISM ; ORTHOPTERA ; POPULATIONS ; ACRIDIDAE ; IDENTIFICATION ; BEHAVIOR ; ORIGIN ; PUNJAB
WOS类目	Zoology
WOS研究方向	Zoology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/327599
作者单位	[Ahmad, Jam Nazeer; Ahmad, Samina J. N.; Malik, Mubashir Ahmad; Ali, Abid; Ahmad, Ejaz] Univ Agr Faisalabad, Dept Entomol, Faisalabad, Pakistan; [Ahmad, Samina J. N.; Tahir, Muhammad; Ashraf, Muhammad] Univ Agr Faisalabad, Dept Bot, Plant Stress Physiol & Mol Biol Lab, Faisalabad, Pakistan; [Ali, Muhammad] Univ Punjab, Inst Agr Sci, Quaid e Azam Campus, Lahore, Pakistan
推荐引用方式 GB/T 7714	Ahmad, Jam Nazeer,Ahmad, Samina J. N.,Malik, Mubashir Ahmad,et al. Molecular Evidence for the Association of Swarm Forming Desert Locust, Schistocerca gregaria gregaria (Forskal) in Pakistan with Highly Prevalent Subspecies in Sahara Desert of Africa[J],2020,52(6):2233-2242.
APA	Ahmad, Jam Nazeer.,Ahmad, Samina J. N..,Malik, Mubashir Ahmad.,Ali, Abid.,Ali, Muhammad.,...&Ashraf, Muhammad.(2020).Molecular Evidence for the Association of Swarm Forming Desert Locust, Schistocerca gregaria gregaria (Forskal) in Pakistan with Highly Prevalent Subspecies in Sahara Desert of Africa.PAKISTAN JOURNAL OF ZOOLOGY,52(6),2233-2242.
MLA	Ahmad, Jam Nazeer,et al."Molecular Evidence for the Association of Swarm Forming Desert Locust, Schistocerca gregaria gregaria (Forskal) in Pakistan with Highly Prevalent Subspecies in Sahara Desert of Africa".PAKISTAN JOURNAL OF ZOOLOGY 52.6(2020):2233-2242.