干旱区生态环境知识资源中心(Arid): Alzheimer's Disease-Like Pathology Triggered by Porphyromonas gingivalis in Wild Type Rats Is Serotype Dependent

Arid

DOI	10.3389/fimmu.2020.588036
	Alzheimer's Disease-Like Pathology Triggered by Porphyromonas gingivalis in Wild Type Rats Is Serotype Dependent
	Diaz-Zuniga, Jaime; More, Jamileth; Melgar-Rodriguez, Samanta; Jimenez-Union, Matias; Villalobos-Orchard, Francisca; Munoz-Manriquez, Constanza; Monasterio, Gustavo; Luis Valdes, Jose; Vernal, Rolando; Paula-Lima, Andrea
通讯作者	Paula-Lima, A
来源期刊	FRONTIERS IN IMMUNOLOGY
ISSN	1664-3224
出版年	2020
卷号	11
英文摘要	Periodontal disease is a disease of tooth-supporting tissues. It is a chronic disease with inflammatory nature and infectious etiology produced by a dysbiotic subgingival microbiota that colonizes the gingivodental sulcus. Among several periodontal bacteria, Porphyromonas gingivalis (P. gingivalis) highlights as a keystone pathogen. Previous reports have implied that chronic inflammatory response and measurable bone resorption are observed in young mice, even after a short period of periodontal infection with P. gingivalis, which has been considered as a suitable model of experimental periodontitis. Also, encapsulated P. gingivalis strains are more virulent than capsular-defective mutants, causing an increased immune response, augmented osteoclastic activity, and accrued alveolar bone resorption in these rodent experimental models of periodontitis. Recently, P. gingivalis has been associated with Alzheimer's disease (AD) pathogenesis, either by worsening brain pathology in AD-transgenic mice or by inducing memory impairment and age-dependent neuroinflammation middle-aged wild type animals. We hypothesized here that the more virulent encapsulated P. gingivalis strains could trigger the appearance of brain AD-markers, neuroinflammation, and cognitive decline even in young rats subjected to a short periodontal infection exposure, due to their higher capacity of activating brain inflammatory responses. To test this hypothesis, we periodontally inoculated 4-week-old male Sprague-Dawley rats with K1, K2, or K4 P. gingivalis serotypes and the K1-isogenic non-encapsulated mutant (GPA), used as a control. 45-days after periodontal inoculations with P. gingivalis serotypes, rat ' s spatial memory was evaluated for six consecutive days in the Oasis maze task. Following functional testing, the animals were sacrificed, and various tissues were removed to analyze alveolar bone resorption, cytokine production, and detect AD-specific biomarkers. Strikingly, only K1 or K2 P. gingivalis-infected rats displayed memory deficits, increased alveolar bone resorption, pro-inflammatory cytokine production, changes in astrocytic morphology, increased A beta 1-42 levels, and Tau hyperphosphorylation in the hippocampus. None of these effects were observed in rats infected with the non-encapsulated bacterial strains. Based on these results, we propose that the bacterial virulence factors constituted by capsular polysaccharides play a central role in activating innate immunity and inflammation in the AD-like pathology triggered by P. gingivalis in young rats subjected to an acute experimental infection episode.
英文关键词	periodontitis Alzheimer’ s disease memory glia neuroinflammation
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000591309000001
WOS关键词	CYSTEINE PROTEINASES GINGIPAINS ; AMYLOID-BETA OLIGOMERS ; 6 CAPSULAR SEROTYPES ; ARG-GINGIPAIN ; LIPOPOLYSACCHARIDE ; PERIODONTITIS ; POLYSACCHARIDE ; PATHOGENESIS ; ACTIVATION ; MICROGLIA
WOS类目	Immunology
WOS研究方向	Immunology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/327582
作者单位	[Diaz-Zuniga, Jaime; Melgar-Rodriguez, Samanta; Jimenez-Union, Matias; Villalobos-Orchard, Francisca; Munoz-Manriquez, Constanza; Monasterio, Gustavo; Vernal, Rolando] Univ Chile, Fac Dent, Periodontal Biol Lab, Santiago, Chile; [More, Jamileth] Hosp Clin Univ Chile, Ctr Invest Clin Avanzada CICA, Santiago, Chile; [Luis Valdes, Jose; Paula-Lima, Andrea] Univ Chile, Biomed Neurosci Inst, Fac Med, Santiago, Chile; [Luis Valdes, Jose; Paula-Lima, Andrea] Univ Chile, Fac Med, Dept Neurosci, Santiago, Chile; [Paula-Lima, Andrea] Univ Chile, Fac Dent, Inst Res Dent Sci, Santiago, Chile
推荐引用方式 GB/T 7714	Diaz-Zuniga, Jaime,More, Jamileth,Melgar-Rodriguez, Samanta,et al. Alzheimer's Disease-Like Pathology Triggered by Porphyromonas gingivalis in Wild Type Rats Is Serotype Dependent[J],2020,11.
APA	Diaz-Zuniga, Jaime.,More, Jamileth.,Melgar-Rodriguez, Samanta.,Jimenez-Union, Matias.,Villalobos-Orchard, Francisca.,...&Paula-Lima, Andrea.(2020).Alzheimer's Disease-Like Pathology Triggered by Porphyromonas gingivalis in Wild Type Rats Is Serotype Dependent.FRONTIERS IN IMMUNOLOGY,11.
MLA	Diaz-Zuniga, Jaime,et al."Alzheimer's Disease-Like Pathology Triggered by Porphyromonas gingivalis in Wild Type Rats Is Serotype Dependent".FRONTIERS IN IMMUNOLOGY 11(2020).