Arid
DOI10.1016/j.jksus.2020.10.003
Assessment of Inter simple sequence repeat (ISSR) and simple sequence repeat (SSR) markers to reveal genetic diversity among Tamarix ecotypes
Ramzan, Musarrat; Sarwar, Sadia; Kauser, Naheed; Saba, Rabia; Hussain, Iqtidar; Shah, Anis Ali; Aslam, Muhammad Naveed; Alkahtani, Jawaher; Alwahibi, Mona S.
通讯作者Ramzan, M
来源期刊JOURNAL OF KING SAUD UNIVERSITY SCIENCE
ISSN1018-3647
EISSN2213-686X
出版年2020
卷号32期号:8页码:3437-3446
英文摘要Tamarix is an important genus of Tamarieaceae family, known for its medicinal importance. Its taxonomy is tricky, and a few molecular makers are available for species identification. The present study was designed to evaluate the genetic diversity among 21 Tamarix specimens collected from Thal desert, Punjab, Pakistan. Ten Inter-Simple Sequence Repeats (ISSR) and six Simple Sequence Repeats (SSR) mark-ers were used. Results revealed that ISSR markers produced 131 bands, of which 116 were polymorphic (88.5%) with a mean Polymorphic Information Content (PIC) value of 0.34. The SSR markers yielded 30 amplified bands, of which 24 were polymorphic (80.28%) with mean PIC value of 0.35. Cluster and factorial analysis revealed a high level of genetic variability among tested Tamarix ecotypes. Dissimilarity index indicated a genetic variability among tested specimens ranging from 0.00 to 0.77. The ISSR analysis reflected that Tamarix specimens stemming from Kalurkot and Bhakkar region revealed the highest dissimilarity level with respect to the rest of the specimens. (c) 2020 The Authors. Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
英文关键词Thal area Plant taxonomy Microsatellite Inter simple sequence repeat Genetic diversity
类型Article
语种英语
开放获取类型gold
收录类别SCI-E
WOS记录号WOS:000590879300007
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
来源机构King Saud University
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/327568
作者单位[Ramzan, Musarrat] Islamia Univ Bahawalpur, Dept Bot, Bahawalpur, Pakistan; [Sarwar, Sadia; Saba, Rabia; Shah, Anis Ali] Univ Sargodha, Dept Biol Sci, Sub Campus Bhakhar, Bhakkar, Pakistan; [Kauser, Naheed; Hussain, Iqtidar] Jinnah Univ Women Karachi, Dept Biotechnol, Karachi, Pakistan; [Shah, Anis Ali] Univ Narowal, Dept Bot, Narowal, Pakistan; [Aslam, Muhammad Naveed] Islamia Univ Bahawalpur, Dept Plant Pathol, Coll Agr & Environm Sci, Bahawalpur, Pakistan; [Alkahtani, Jawaher; Alwahibi, Mona S.] King Saud Univ, Dept Bot & Microbiol, Coll Sci, Riyadh 11451, Saudi Arabia
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Ramzan, Musarrat,Sarwar, Sadia,Kauser, Naheed,et al. Assessment of Inter simple sequence repeat (ISSR) and simple sequence repeat (SSR) markers to reveal genetic diversity among Tamarix ecotypes[J]. King Saud University,2020,32(8):3437-3446.
APA Ramzan, Musarrat.,Sarwar, Sadia.,Kauser, Naheed.,Saba, Rabia.,Hussain, Iqtidar.,...&Alwahibi, Mona S..(2020).Assessment of Inter simple sequence repeat (ISSR) and simple sequence repeat (SSR) markers to reveal genetic diversity among Tamarix ecotypes.JOURNAL OF KING SAUD UNIVERSITY SCIENCE,32(8),3437-3446.
MLA Ramzan, Musarrat,et al."Assessment of Inter simple sequence repeat (ISSR) and simple sequence repeat (SSR) markers to reveal genetic diversity among Tamarix ecotypes".JOURNAL OF KING SAUD UNIVERSITY SCIENCE 32.8(2020):3437-3446.
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