干旱区生态环境知识资源中心(Arid): Impact of Soil Salinity on the Cowpea Nodule-Microbiome and the Isolation of Halotolerant PGPR Strains to Promote Plant Growth under Salinity Stress

Arid

DOI	10.1094/PBIOMES-09-19-0057-R
	Impact of Soil Salinity on the Cowpea Nodule-Microbiome and the Isolation of Halotolerant PGPR Strains to Promote Plant Growth under Salinity Stress
	Mukhtar, Salma; Hirsch, Ann M.; Khan, Noor; Malik, Kauser A.; Humm, Ethan A.; Pellegrini, Matteo; Shi, Baochen; Briscoe, Leah; Huntemann, Marcel; Clum, Alicia; Foster, Brian; Foster, Bryce; Roux, Simon; Palaniappan, Krishnaveni; Varghese, Neha; Mukherjee, Supratim; Reddy, T. B. K.; Daum, Chris; Copeland, Alex; Ivanova, Natalia N.; Kyrpides, Nikos C.; Shapiro, Nicole; Eloe-Fadrosh, Emiley A.; Maymon, Maskit; Mirza, Muhammad S.; Mehnaz, Samina
通讯作者	Hirsch, AM
来源期刊	PHYTOBIOMES JOURNAL
EISSN	2471-2906
出版年	2020
卷号	4 期号:4 页码:364-374
英文摘要	Cowpea is one of the major legumes cultivated in arid and semiarid regions of the world. Four soil-microbial samples (SS-1 through SS-4) collected from semiarid soils in Punjab, Pakistan were planted with cowpea (Vigna unguiculata) crops, which were grown under salinity stress to analyze bacterial composition in the rhizosphere and within nodules using cultivation-dependent and -independent methods. Two varieties, 603 and the salt-tolerant CB 46, were each inoculated with or without the four different native soil samples or grown in medium either N-deficient ( N) or supplemented with N (+N). Plants inoculated with soil samples SS2 and SS-4 grew better than plants inoculated with SS-1- and SS-3 and grew comparably with the +N controls. Environmental DNA (eDNA) was isolated from SS-1 and SS-4, and, by 16S ribosomal RNA sequencing, the soil microbiomes consisted mainly of Actinobacteria, Firmicutes, Proteobacteria, and other nonproteobacterial genera. However, analysis of eDNA isolated from cowpea nodules established by the trap plants showed that the the nodule microbiome consisted almost exclusively of proteobacterial sequences, particularly species of Bradyrhizobium. Bacteria were isolated from both soils and nodules, and 34 of the 51 isolates tested positive for plant-growth-promoting rhizobacteria traits in plate assays. Many could serve as future inocula for crops in arid soils. The discrepancy between the types of bacteria isolated by culturing bacteria isolated from surface-sterilized cowpea nodules (proteobacteria and nonproteobacteria) versus those detected by sequencing DNA isolated from the nodules (proteobacteria) from cowpea nodules (proteobacteria and nonproteobacteria) versus those detected in the nodule microbiome (proteobacteria) needs further study.
英文关键词	cowpea microbiome halotolerant plant-growth-promoting bacteria/rhizobacteria metagenomics microbiome natural habitats salinity stress symbiosis
类型	Article
语种	英语
开放获取类型	Green Submitted, gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000589975800007
WOS关键词	SYMBIOTIC NITROGEN-FIXATION ; ENDOPHYTIC BACTERIA ; LEGUME NODULES ; DIVERSITY ; COMMUNITIES ; METABOLITES ; PATHOGENS ; DATABASE ; RANGE ; NOV
WOS类目	Plant Sciences ; Microbiology
WOS研究方向	Plant Sciences ; Microbiology
来源机构	University of California, Los Angeles
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/327540
作者单位	[Mukhtar, Salma; Hirsch, Ann M.; Khan, Noor; Humm, Ethan A.; Pellegrini, Matteo; Maymon, Maskit] Univ Calif Los Angeles UCLA, Dept Mol Cell & Dev Biol, 621 Charles Young Dr South, Los Angeles, CA 90095 USA; [Mukhtar, Salma; Malik, Kauser A.; Mehnaz, Samina] Forman Christian Coll, Sch Life Sci, Ferozepur Rd, Lahore 54600, Pakistan; [Hirsch, Ann M.; Pellegrini, Matteo] Univ Calif Los Angeles, Mol Biol Inst, 621 Charles Young Dr South, Los Angeles, CA 90095 USA; [Shi, Baochen] Univ Calif Los Angeles, Dept Mol & Med Pharmacol, David Geffen Sch Med, 621 Charles Young Dr South, Los Angeles, CA 90095 USA; [Briscoe, Leah] Univ Calif Los Angeles, Dept Bioinformat, 621 Charles Young Dr South, Los Angeles, CA 90095 USA; [Huntemann, Marcel; Clum, Alicia; Foster, Brian; Foster, Bryce; Roux, Simon; Palaniappan, Krishnaveni; Varghese, Neha; Mukherjee, Supratim; Reddy, T. B. K.; Daum, Chris; Copeland, Alex; Ivanova, Natalia N.; Kyrpides, Nikos C.; Shapiro, Nicole; Eloe-Fadrosh, Emiley A.] US DOE, Joint Genome Inst, Lawre...
推荐引用方式 GB/T 7714	Mukhtar, Salma,Hirsch, Ann M.,Khan, Noor,et al. Impact of Soil Salinity on the Cowpea Nodule-Microbiome and the Isolation of Halotolerant PGPR Strains to Promote Plant Growth under Salinity Stress[J]. University of California, Los Angeles,2020,4(4):364-374.
APA	Mukhtar, Salma.,Hirsch, Ann M..,Khan, Noor.,Malik, Kauser A..,Humm, Ethan A..,...&Mehnaz, Samina.(2020).Impact of Soil Salinity on the Cowpea Nodule-Microbiome and the Isolation of Halotolerant PGPR Strains to Promote Plant Growth under Salinity Stress.PHYTOBIOMES JOURNAL,4(4),364-374.
MLA	Mukhtar, Salma,et al."Impact of Soil Salinity on the Cowpea Nodule-Microbiome and the Isolation of Halotolerant PGPR Strains to Promote Plant Growth under Salinity Stress".PHYTOBIOMES JOURNAL 4.4(2020):364-374.