干旱区生态环境知识资源中心(Arid): Exopolysaccharides Producing Bacteria for the Amelioration of Drought Stress in Wheat

Arid

DOI	10.3390/su12218876
	Exopolysaccharides Producing Bacteria for the Amelioration of Drought Stress in Wheat
	Ilyas, Noshin; Mumtaz, Komal; Akhtar, Nosheen; Yasmin, Humaira; Sayyed, R. Z.; Khan, Wajiha; El Enshasy, Hesham A.; Dailin, Daniel J.; Elsayed, Elsayed A.; Ali, Zeshan
通讯作者	Ilyas, N
来源期刊	SUSTAINABILITY
EISSN	2071-1050
出版年	2020
卷号	12 期号:21
英文摘要	This research was designed to elucidate the role of exopolysaccharides (EPS) producing bacterial strains for the amelioration of drought stress in wheat. Bacterial strains were isolated from a farmer's field in the arid region of Pakistan. Out of 24 isolated stains, two bacterial strains, Bacillus subtilis (Accession No. MT742976) and Azospirillum brasilense (Accession No. MT742977) were selected, based on their ability to produce EPS and withstand drought stress. Both bacterial strains produced a good amount of EPS and osmolytes and exhibited drought tolerance individually, however, a combination of these strains produced higher amounts of EPS (sugar 6976 mu g/g, 731.5 mu g/g protein, and 1.1 mg/g uronic acid) and osmolytes (proline 4.4 mu g/mg and sugar 79 mu g/mg) and significantly changed the level of stress-induced phytohormones (61%, 49% and 30% decrease in Indole Acetic Acid (IAA), Gibberellic Acid (GA), and Cytokinin (CK)) respectively under stress, but an increase of 27.3% in Abscisic acid (ABA) concentration was observed. When inoculated, the combination of these strains improved seed germination, seedling vigor index, and promptness index by 18.2%, 23.7%, and 61.5% respectively under osmotic stress (20% polyethylene glycol, PEG6000). They also promoted plant growth in a pot experiment with an increase of 42.9%, 29.8%, and 33.7% in shoot length, root length, and leaf area, respectively. Physiological attributes of plants were also improved by bacterial inoculation showing an increase of 39.8%, 61.5%, and 45% in chlorophyll a, chlorophyll b, and carotenoid content respectively, as compared to control. Inoculations of bacterial strains also increased the production of osmolytes such asproline, amino acid, sugar, and protein by 30%, 23%, 68%, and 21.7% respectively. Co-inoculation of these strains enhanced the production of antioxidant enzymes such as superoxide dismutase (SOD) by 35.1%, catalase (CAT) by 77.4%, and peroxidase (POD) by 40.7%. Findings of the present research demonstrated that EPS, osmolyte, stress hormones, and antioxidant enzyme-producing bacterial strains impart drought tolerance in wheat and improve its growth, morphological attributes, physiological parameters, osmolytes production, and increase antioxidant enzymes.
英文关键词	antioxidant enzymes Azospirillum brasilense Bacillus subtilis stress hormones osmolytes plant growth promotion wheat
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E ; SSCI
WOS记录号	WOS:000589277100001
WOS关键词	GROWTH-PROMOTING RHIZOBACTERIA ; SUPEROXIDE-DISMUTASE ; COLORIMETRIC METHOD ; GENE-EXPRESSION ; PLANT ; TOLERANCE ; YIELD ; QUANTITATION ; INOCULATION ; PEROXIDASE
WOS类目	Green & Sustainable Science & Technology ; Environmental Sciences ; Environmental Studies
WOS研究方向	Science & Technology - Other Topics ; Environmental Sciences & Ecology
来源机构	King Saud University
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/327504
作者单位	[Ilyas, Noshin; Mumtaz, Komal; Akhtar, Nosheen] PMAS Arid Agr Univ, Dept Bot, Rawalpindi 46300, Pakistan; [Yasmin, Humaira] COMSATS Univ, Dept Biosci, Islamabad 45550, Pakistan; [Sayyed, R. Z.] PSGVP Mandals Arts Sci & Commerce Coll, Dept Microbiol, Shahada 425409, Maharashtra, India; [Khan, Wajiha] COMSATS Univ Islamabad, Dept Biotechnol, Abbottabad Campus, Abbottabad 22010, Pakistan; [El Enshasy, Hesham A.; Dailin, Daniel J.] Univ Teknol Malaysia UTM, Inst Bioprod Dev IBD, Skudai 81310, Johor, Malaysia; [El Enshasy, Hesham A.; Dailin, Daniel J.] Univ Teknol Malaysia UTM, Fac Engn, Sch Chem & Energy Engn, Skudai 81310, Johor, Malaysia; [El Enshasy, Hesham A.] City Sci Res & Technol Applicat SRTA, Alexandria 21934, Egypt; [Elsayed, Elsayed A.] King Saud Univ, Coll Sci, Zool Dept, PO 2455, Riyadh 11451, Saudi Arabia; [Elsayed, Elsayed A.] Natl Res Ctr, Chem Nat & Microbial Prod Dept, Cairo 11651, Egypt; [Ali, Zeshan] Natl Agr Res Ctr, Crop Sci Inst, Plant Physiol Program, Pk Rd, Islamabad 45500, Pakistan
推荐引用方式 GB/T 7714	Ilyas, Noshin,Mumtaz, Komal,Akhtar, Nosheen,et al. Exopolysaccharides Producing Bacteria for the Amelioration of Drought Stress in Wheat[J]. King Saud University,2020,12(21).
APA	Ilyas, Noshin.,Mumtaz, Komal.,Akhtar, Nosheen.,Yasmin, Humaira.,Sayyed, R. Z..,...&Ali, Zeshan.(2020).Exopolysaccharides Producing Bacteria for the Amelioration of Drought Stress in Wheat.SUSTAINABILITY,12(21).
MLA	Ilyas, Noshin,et al."Exopolysaccharides Producing Bacteria for the Amelioration of Drought Stress in Wheat".SUSTAINABILITY 12.21(2020).