干旱区生态环境知识资源中心(Arid): GABA metabolism is crucial for long-term survival of anoxia in annual killifish embryos

Arid

DOI	10.1242/jeb.229715
	GABA metabolism is crucial for long-term survival of anoxia in annual killifish embryos
	Zajic, Daniel E.; Podrabsky, Jason E.
通讯作者	Zajic, DE
来源期刊	JOURNAL OF EXPERIMENTAL BIOLOGY
ISSN	0022-0949
EISSN	1477-9145
出版年	2020
卷号	223 期号:20
英文摘要	In most vertebrates, a lack of oxygen quickly leads to irreparable damages to vital organs, such as the brain and heart. However, there are some vertebrates that have evolved mechanisms to survive periods of no oxygen (anoxia). The annual killifish (Austrofundulus limnaeus) survives in ephemeral ponds in the coastal deserts of Venezuela and their embryos have the remarkable ability to tolerate anoxia for months. When exposed to anoxia, embryos of A. limnaeus respond by producing significant amounts of y-aminobutyric acid (GABA). This study aims to understand the role of GABA in supporting the metabolic response to anoxia. To explore this, we investigated four developmentally distinct stages of A. limnaeus embryos that vary in their anoxia tolerance. We measured GABA and lactate concentrations across development in response to anoxia and aerobic recovery. We then inhibited enzymes responsible for the production and degradation of GABA and observed GABA and lactate concentrations, as well as embryo mortality. Here, we show for the first time that GABA metabolism affects anoxia tolerance in A. limnaeus embryos. Inhibition of enzymes responsible for GABA production (glutamate decarboxylase) and degradation (GABA-transaminase and succinic acid semialdehyde dehydrogenase) led to increased mortality, supporting a role for GABA as an intermediate product and not a metabolic end-product. We propose multiple roles for GABA during anoxia and aerobic recovery in A. limnaeus embryos, serving as a neurotransmitter, an energy source, and an anti-oxidant.
英文关键词	Aerobic recovery Anoxia Austrofundulus limnaeus GABA Lactate Metabolism
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000589086900017
WOS关键词	CHRYSEMYS-PICTA-BELLII ; AUSTROFUNDULUS-LIMNAEUS ; DEVELOPMENTAL BIOLOGY ; TRANSAMINASE INHIBITION ; GENE-EXPRESSION ; ANNUAL FISHES ; CELL-DEATH ; IN-VITRO ; DIAPAUSE ; TOLERANCE
WOS类目	Biology
WOS研究方向	Life Sciences & Biomedicine - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/327487
作者单位	[Zajic, Daniel E.; Podrabsky, Jason E.] Portland State Univ, Dept Biol, POB 751, Portland, OR 97207 USA; [Zajic, Daniel E.] Linfield Univ, Hlth Human Performance & Athlet Dept, 900 SE Baker, Mcminnville, OR 97128 USA
推荐引用方式 GB/T 7714	Zajic, Daniel E.,Podrabsky, Jason E.. GABA metabolism is crucial for long-term survival of anoxia in annual killifish embryos[J],2020,223(20).
APA	Zajic, Daniel E.,&Podrabsky, Jason E..(2020).GABA metabolism is crucial for long-term survival of anoxia in annual killifish embryos.JOURNAL OF EXPERIMENTAL BIOLOGY,223(20).
MLA	Zajic, Daniel E.,et al."GABA metabolism is crucial for long-term survival of anoxia in annual killifish embryos".JOURNAL OF EXPERIMENTAL BIOLOGY 223.20(2020).