干旱区生态环境知识资源中心(Arid): Suaeda vermiculata Aqueous-Ethanolic Extract-Based Mitigation of CCl4-Induced Hepatotoxicity in Rats, and HepG-2 and HepG-2/ADR Cell-Lines-Based Cytotoxicity Evaluations

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DOI	10.3390/plants9101291
	Suaeda vermiculata Aqueous-Ethanolic Extract-Based Mitigation of CCl4-Induced Hepatotoxicity in Rats, and HepG-2 and HepG-2/ADR Cell-Lines-Based Cytotoxicity Evaluations
	Mohammed, Salman A. A.; Khan, Riaz A.; El-Readi, Mahmoud Z.; Emwas, Abdul-Hamid; Sioud, Salim; Poulson, Benjamin G.; Jaremko, Mariusz; Eldeeb, Hussein M.; Al-Omar, Mohsen S.; Mohammed, Hamdoon A.
通讯作者	Mohammed, SAA
来源期刊	PLANTS-BASEL
EISSN	2223-7747
出版年	2020
卷号	9 期号:10
英文摘要	Suaeda vermiculata, an edible halophytic plant, used by desert nomads to treat jaundice, was investigated for its hepatoprotective bioactivity and safety profile on its mother liquor aqueous-ethanolic extract. Upon LC-MS (Liquid Chromatography-Mass Spectrometry) analysis, the presence of several constituents including three major flavonoids, namely quercetin, quercetin-3-O-rutinoside, and kaempferol-O-(acetyl)-hexoside-pentoside were confirmed. The aqueous-ethanolic extract, rich in antioxidants, quenched the DPPH (1,1-diphenyl-2-picrylhydrazyl) radicals, and also showed noticeable levels of radical scavenging capacity in ABTS (2,2 '-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid) assay. For the hepatoprotective activity confirmation, the male rat groups were fed daily, for 7 days (n = 8/group, p.o.), either carboxyl methylcellulose (CMC) 0.5%, silymarin 200 mg/kg, the aqueous-ethanolic extract of the plant Suaeda vermiculata (100, 250, and 500 mg/kg extract), or quercetin (100 mg/kg) alone, and on day 7 of the administrations, all the animal groups, excluding a naive (250 mg/kg aqueous-ethanolic extract-fed), and an intact animal group were induced hepatotoxicity by intraperitoneally administering carbon tetrachloride (CCl4). All the animals were sacrificed after 24 h, and aspartate transaminase and alanine transaminase serum levels were observed, which were noted to be significantly decreased for the aqueous-ethanolic extract, silymarin, and quercetin-fed groups in comparison to the CMC-fed group (p < 0.0001). No noticeable adverse effects were observed on the liver, kidney, or heart's functions of the naive (250 mg/kg) group. The aqueous-ethanolic extract was found to be safe in the acute toxicity (5 g/kg) test and showed hepatoprotection and safety at higher doses. Further upon, the cytotoxicity testings in HepG-2 and HepG-2/ADR (Adriamycin resistant) cell-lines were also investigated, and the IC50 values were recorded at 56.19 +/- 2.55 mu g/mL, and 78.40 +/- 0.32 mu g/mL (p < 0.001, Relative Resistance RR 1.39), respectively, while the doxorubicin (Adriamycin) IC50 values were found to be 1.3 +/- 0.064, and 4.77 +/- 1.05 mu g/mL (p < 0.001, RR 3.67), respectively. The HepG-2/ADR cell-lines when tested in a combination of the aqueous-ethanolic extract with doxorubicin, a significant reversal in the doxorubicin's IC50 value by 2.77 folds (p < 0.001, CI = 0.56) was noted as compared to the cytotoxicity test where the extract was absent. The mode of action for the reversal was determined to be synergistic in nature indicating the role of the aqueous-ethanolic extract.
英文关键词	Suaeda vermiculata halophyte aqueous-ethanolic extract antioxidant liver toxicity cytotoxicity hepatoprotective liver disorders mass spectrometry LC-MS HepG-2 HepG-2/ADR
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000586274000001
WOS关键词	TETRACHLORIDE-INDUCED HEPATOTOXICITY ; INDUCED LIVER FIBROSIS ; OXIDATIVE STRESS ; HEPATOPROTECTIVE ACTIVITY ; ANTIOXIDANT ACTIVITIES ; BIOLOGICAL-ACTIVITIES ; ANIMAL-MODELS ; VITAMIN-C ; QUERCETIN ; SILYMARIN
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/327337
作者单位	[Mohammed, Salman A. A.; Eldeeb, Hussein M.] Qassim Univ, Coll Pharm, Dept Pharmacol & Toxicol, Qasim 51452, Saudi Arabia; [Khan, Riaz A.; Al-Omar, Mohsen S.; Mohammed, Hamdoon A.] Qassim Univ, Coll Pharm, Dept Med Chem & Pharmacognosy, Qasim 51452, Saudi Arabia; [El-Readi, Mahmoud Z.] Umm Al Qura Univ, Fac Med, Dept Clin Biochem, Mecca 21955, Saudi Arabia; [El-Readi, Mahmoud Z.] Al Azhar Univ, Fac Pharm, Dept Biochem, Assiut 71524, Egypt; [Emwas, Abdul-Hamid; Sioud, Salim] King Abdullah Univ Sci & Technol KAUST, Core Labs, Thuwal 239556900, Saudi Arabia; [Poulson, Benjamin G.; Jaremko, Mariusz] King Abdullah Univ Sci & Technol KAUST, Biol & Environm Sci & Engn Div BESE, Thuwal 239556900, Saudi Arabia; [Eldeeb, Hussein M.] Al Azhar Univ, Fac Med, Dept Biochem, Assiut 71524, Egypt; [Al-Omar, Mohsen S.] JUST, Med Chem & Pharmacognosy Dept, Fac Pharm, Irbid 22110, Jordan; [Mohammed, Hamdoon A.] Al Azhar Univ, Fac Pharm, Dept Pharmacognosy, Cairo 11371, Egypt
推荐引用方式 GB/T 7714	Mohammed, Salman A. A.,Khan, Riaz A.,El-Readi, Mahmoud Z.,et al. Suaeda vermiculata Aqueous-Ethanolic Extract-Based Mitigation of CCl4-Induced Hepatotoxicity in Rats, and HepG-2 and HepG-2/ADR Cell-Lines-Based Cytotoxicity Evaluations[J],2020,9(10).
APA	Mohammed, Salman A. A..,Khan, Riaz A..,El-Readi, Mahmoud Z..,Emwas, Abdul-Hamid.,Sioud, Salim.,...&Mohammed, Hamdoon A..(2020).Suaeda vermiculata Aqueous-Ethanolic Extract-Based Mitigation of CCl4-Induced Hepatotoxicity in Rats, and HepG-2 and HepG-2/ADR Cell-Lines-Based Cytotoxicity Evaluations.PLANTS-BASEL,9(10).
MLA	Mohammed, Salman A. A.,et al."Suaeda vermiculata Aqueous-Ethanolic Extract-Based Mitigation of CCl4-Induced Hepatotoxicity in Rats, and HepG-2 and HepG-2/ADR Cell-Lines-Based Cytotoxicity Evaluations".PLANTS-BASEL 9.10(2020).