干旱区生态环境知识资源中心(Arid): Molecular Characterizations of Double-Stranded RNA Degrading Nuclease Genes from Ostrinia nubilalis

Arid

DOI	10.3390/insects11100652
	Molecular Characterizations of Double-Stranded RNA Degrading Nuclease Genes from Ostrinia nubilalis
	Cooper, Anastasia M. W.; Song, Huifang; Shi, Xuekai; Yu, Zhitao; Lorenzen, Marce; Silver, Kristopher; Zhang, Jianzhen; Zhu, Kun Yan
通讯作者	Cooper, AMW ; Zhu, KY
来源期刊	INSECTS
EISSN	2075-4450
出版年	2020
卷号	11 期号:10
英文摘要	Simple Summary RNA interference is a gene suppression tool that uses double-stranded RNA to prevent specific genes from producing proteins. By targeting essential genes RNA interference can be developed for control of insect pests. Unfortunately, RNA interference is not equally effective for all insects. Previous investigation suggested that RNA is rapidly digested by unidentified components of body fluids in the European corn borer caterpillar. We characterized genes encoding proteins from European corn borer that are associated with RNA digestion in other insects. Our results suggest that two proteins (RNA interference efficiency-related nuclease and double-stranded RNA-degrading endonuclease 2) may be responsible for digesting RNAs in the European corn borer gut, whereas two other proteins (double-stranded RNA-degrading endonuclease 1 and double-stranded RNA-degrading endonuclease 4) may be responsible for digesting RNA in European corn borer body fluid. These findings suggest digestion of RNA in the European corn borer is likely due to the activity of these proteins. These findings provide information about the mechanism(s) influencing RNA stability in insects. The knowledge generated by this study will facilitate the development of strategies for enhancing RNA interference in insects. Variable RNA interference (RNAi) efficiencies limit RNAi-based pest management strategies for many pests. Previous efforts to understand mechanisms contributing to low RNAi efficiency indicate that double-stranded RNA (dsRNA) is degraded in the European corn borer (ECB), Ostrinia nubilalis, due to nuclease activity. To investigate the contribution of dsRNA-degrading endonucleases (dsRNases) and lepidopteran-specific RNAi efficiency-related nucleases (REases) to dsRNA instability and low RNAi efficiency in ECB, five complementary DNAs putatively encoding four dsRNases (OndsRNase1, 2, 3, and 4) and one REase (OnREase) were sequenced. Characterization of these transcripts revealed that substrate specificity might vary among the four dsRNases due to different amino acid combinations in the substrate-binding sites. Gene expression analysis indicated that OndsRNase2 and OnREase were highly expressed in the larval gut, and OndsRNase1 showed the highest expression in hemolymph, especially in older developmental stages. Transcript level analysis after dsRNA exposure revealed that expression of OnREase rapidly increased upon dsRNA ingestion or injection, whereas OndsRNase4 expression only increased after long-term ingestion of dsRNA. While the biological function of these nucleases remains to be verified, our results suggest that OnREase and OndsRNase2, and OndsRNase1 and OndsRNase4 may be responsible for degradation of dsRNAs in the ECB gut and hemolymph, respectively, thereby contributing to low RNAi efficiency.
英文关键词	dsRNase European corn borer gene expression REase RNAi efficiency substrate specificity
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000586250500001
WOS关键词	COLORADO POTATO BEETLE ; SUBCELLULAR-LOCALIZATION ; DESERT LOCUST ; INTERFERENCE ; EXPRESSION ; GUT ; RESISTANCE ; EFFICIENCY ; DSRNA ; PCR
WOS类目	Entomology
WOS研究方向	Entomology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/327334
作者单位	[Cooper, Anastasia M. W.; Song, Huifang; Shi, Xuekai; Yu, Zhitao; Silver, Kristopher; Zhang, Jianzhen; Zhu, Kun Yan] Kansas State Univ, Dept Entomol, 123 Waters Hall, Manhattan, KS 66506 USA; [Song, Huifang; Shi, Xuekai; Zhang, Jianzhen] Shanxi Univ, Inst Appl Biol, Taiyuan 030006, Peoples R China; [Lorenzen, Marce] North Carolina State Univ, Dept Entomol & Plant Pathol, Campus Box 7613, Raleigh, NC 27695 USA
推荐引用方式 GB/T 7714	Cooper, Anastasia M. W.,Song, Huifang,Shi, Xuekai,et al. Molecular Characterizations of Double-Stranded RNA Degrading Nuclease Genes from Ostrinia nubilalis[J],2020,11(10).
APA	Cooper, Anastasia M. W..,Song, Huifang.,Shi, Xuekai.,Yu, Zhitao.,Lorenzen, Marce.,...&Zhu, Kun Yan.(2020).Molecular Characterizations of Double-Stranded RNA Degrading Nuclease Genes from Ostrinia nubilalis.INSECTS,11(10).
MLA	Cooper, Anastasia M. W.,et al."Molecular Characterizations of Double-Stranded RNA Degrading Nuclease Genes from Ostrinia nubilalis".INSECTS 11.10(2020).