干旱区生态环境知识资源中心(Arid): A Novel Arthropod Host of Brucellosis in the Arid Steppe Ecosystem

Arid

DOI	10.3389/fvets.2020.566253
	A Novel Arthropod Host of Brucellosis in the Arid Steppe Ecosystem
	Huang, Tianpeng; Zhang, Jinbao; Sun, Changyun; Liu, Zhicheng; He, Haiyan; Wu, Jie; Geriletu
通讯作者	Geriletu
来源期刊	FRONTIERS IN VETERINARY SCIENCE
EISSN	2297-1769
出版年	2020
卷号	7
英文摘要	Brucellosis is a severe public health problem in the Inner Mongolia regions of China. The recent prevalence of brucellosis outbreaks may be attributed to an increase in the activity of ticks and other air-borne vectors. Dermacentor nuttalli (D. nuttalli) is a native tick species of Inner Mongolia; similar to other tick species, D. nuttalli carries a variety of pathogens that can be transmitted to a wide range of animals. In this study, we have investigated the potential of D. nuttalli in transmitting brucellosis. From 2015 to 2019, 2,256 ticks were collected from 23 different pastoral areas of Hulun Buir. Brucellosis pathogen was detected using DNA extracted from different developmental stages of ticks. Salivary gland and midgut tissue samples were used as templates to amplify Brucella Bscp31 gene (Brucella genus-specific gene) by using TaqMan Real-Time polymerase chain reaction (PCR). To detect the presence of Bscp31 protein, which is specific to Brucella spp., in the midgut and salivary gland tissues of D. nuttalli, Western blotting and immunofluorescence were performed. Additionally, Brucella spp. were isolated using a culture medium. Tick samples were identified as D. nuttalli. Different percentages of Brucella genus-specific genes could be found in the tick samples. From 2015 to 2019, the positivity rate for the detection of Bscp31 gene in D. nuttalli ranged from 0.00 to 87.80%, with the highest rate of 89.00%. In addition, Brucella genus-specific genes were successfully detected in the samples isolated from all the developmental stages and anatomical regions of ticks. Bscp31 protein was present in the midgut and salivary gland of D. nuttalli. Further, B. melitensis biotype 3 was isolated from eggs and engorged adults of D. nuttalli. These findings demonstrate that D. nuttalli is a potent, long-term carrier of Brucella spp. that can exhibit transovarial transmission potential, presenting D. nuttalli as a novel arthropod host for Brucella spp. This study, therefore, indicates the potential risk of transmission of brucellosis via tick bites among animals as well as human beings.
英文关键词	brucella D nuttalli developmental stages salivary gland midgut biotype
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000585979800001
WOS关键词	ANAPLASMA-PHAGOCYTOPHILUM ; MOLECULAR-DETECTION ; INNER-MONGOLIA ; TICKS ; EPIDEMIOLOGY ; SPP.
WOS类目	Veterinary Sciences
WOS研究方向	Veterinary Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/327314
作者单位	[Huang, Tianpeng; Liu, Zhicheng; He, Haiyan; Geriletu] Inner Mongolia Agr Univ, Coll Vet Med, Hohhot, Peoples R China; [Zhang, Jinbao] Zhalantun Vocat Coll, Dept Anim Husb & Vet Med, Hulun Buir, Peoples R China; [Sun, Changyun; Wu, Jie] Guangdong Prov Ctr Dis Control & Prevent, Guangzhou, Peoples R China
推荐引用方式 GB/T 7714	Huang, Tianpeng,Zhang, Jinbao,Sun, Changyun,et al. A Novel Arthropod Host of Brucellosis in the Arid Steppe Ecosystem[J],2020,7.
APA	Huang, Tianpeng.,Zhang, Jinbao.,Sun, Changyun.,Liu, Zhicheng.,He, Haiyan.,...&Geriletu.(2020).A Novel Arthropod Host of Brucellosis in the Arid Steppe Ecosystem.FRONTIERS IN VETERINARY SCIENCE,7.
MLA	Huang, Tianpeng,et al."A Novel Arthropod Host of Brucellosis in the Arid Steppe Ecosystem".FRONTIERS IN VETERINARY SCIENCE 7(2020).