干旱区生态环境知识资源中心(Arid): Argonaute4 Modulates Resistance to Fusarium brachygibbosum Infection by Regulating Jasmonic Acid Signaling

Arid

DOI	10.1104/pp.20.00171
	Argonaute4 Modulates Resistance to Fusarium brachygibbosum Infection by Regulating Jasmonic Acid Signaling
	Pradhan, Maitree; Pandey, Priyanka; Baldwin, Ian T.; Pandey, Shree P.
通讯作者	Pandey, SP
来源期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
EISSN	1532-2548
出版年	2020
卷号	184 期号:2 页码:1128-1152
英文摘要	Argonautes (AGOs) associate with noncoding RNAs to regulate gene expression during development and stress adaptation. Their role in plant immunity against hemibiotrophic fungal infection remains poorly understood. Here, we explore the function of AGOs in the interaction of wild tobacco (Nicotiana attenuata) with a naturally occurring hemibiotrophic pathogen, Fusarium brachygibbosum. Among all AGOs, only transcripts of AGO4 were elicited after fungal infection. The disease progressed more rapidly in AGO4-silenced (irAGO4) plants than in wild type, and small RNA (smRNA) profiling revealed that 24-nucleotide smRNA accumulation was severely abrogated in irAGO4 plants. Unique microRNAs (miRNAs: 130 conserved and 208 novel, including 11 canonical miRNA sequence variants known as isomiRs) were identified in infected plants; silencing of AGO4 strongly changed miRNA accumulation dynamics. Time-course studies revealed that infection increased accumulation of abscisic acid, jasmonates, and salicylic acid in wild type; in irAGO4 plants, infection accumulated lower jasmonate levels and lower transcripts of jasmonic acid (JA) biosynthesis genes. Treating irAGO4 plants with JA, methyl jasmonate, or cis-(+)-12-oxo-phytodienoic acid restored wild-type levels of resistance. Silencing expression of RNA-directed RNA polymerases RdR1 and RdR2 (but not RdR3) and Dicer-like3 (DCL3, but not DCL2 or DCL4) increased susceptibility to F. brachygibbosum. The relevance of AGO4, RdR1, RdR2, and DCL3 in a natural setting was revealed when plants individually silenced in their expression (and their binary combinations) were planted in a diseased field plot in the Great Basin Desert of Utah. These plants were more susceptible to infection and accumulated lower JA levels than wild type. We infer that AGO4-dependent smRNAs play a central role in modulating JA biogenesis and signaling during hemibiotrophic fungal infections.
类型	Article
语种	英语
开放获取类型	Green Published
收录类别	SCI-E
WOS记录号	WOS:000585831600054
WOS关键词	NICOTIANA-ATTENUATA ; DEFENSE RESPONSES ; DNA METHYLATION ; SMALL RNAS ; SIRNA ACCUMULATION ; PLANT IMMUNITY ; EXPRESSION ; PROTEINS ; GENES ; ACTIVATION
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/327308
作者单位	[Pradhan, Maitree; Baldwin, Ian T.; Pandey, Shree P.] Max Planck Inst Chem Ecol, Dept Mol Ecol, D-07745 Jena, Germany; [Pandey, Priyanka] Natl Inst Biomed Genom, Kalyani 741251, W Bengal, India; [Pandey, Priyanka] BioNTech AG, D-55131 Mainz, Germany
推荐引用方式 GB/T 7714	Pradhan, Maitree,Pandey, Priyanka,Baldwin, Ian T.,et al. Argonaute4 Modulates Resistance to Fusarium brachygibbosum Infection by Regulating Jasmonic Acid Signaling[J],2020,184(2):1128-1152.
APA	Pradhan, Maitree,Pandey, Priyanka,Baldwin, Ian T.,&Pandey, Shree P..(2020).Argonaute4 Modulates Resistance to Fusarium brachygibbosum Infection by Regulating Jasmonic Acid Signaling.PLANT PHYSIOLOGY,184(2),1128-1152.
MLA	Pradhan, Maitree,et al."Argonaute4 Modulates Resistance to Fusarium brachygibbosum Infection by Regulating Jasmonic Acid Signaling".PLANT PHYSIOLOGY 184.2(2020):1128-1152.