干旱区生态环境知识资源中心(Arid): Integrated analysis of co-expression, conserved genes and gene families reveal core regulatory network of heat stress response inCleistogenes songorica, a xerophyte perennial desert plant

Arid

DOI	10.1186/s12864-020-07122-8
	Integrated analysis of co-expression, conserved genes and gene families reveal core regulatory network of heat stress response inCleistogenes songorica, a xerophyte perennial desert plant
	Yan, Qi; Zong, Xifang; Wu, Fan; Li, Jie; Ma, Tiantian; Zhao, Yufeng; Ma, Qian; Wang, Penglei; Wang, Yanrong; Zhang, Jiyu
通讯作者	Zhang, JY
来源期刊	BMC GENOMICS
ISSN	1471-2164
出版年	2020
卷号	21 期号:1
英文摘要	Background As global warming continues, heat stress (HS) is becoming an increasingly significant factor limiting plant growth and reproduction, especially for cool-season grass species. The objective of this study was to determine the transcriptional regulatory network ofCleistogenes songoricaunder HS via transcriptome profiling, identify of gene families and comparative analysis across major Poaceae species. Results Physiological analysis revealed significantly decreased leaf relative water content (RWC) but increased proline (Pro) content inC. songoricaunder 24 h of HS. Transcriptome profiling indicated that 16,028 and 14,645 genes were differentially expressed in the shoots and roots ofC. songoricaunder HS, respectively. Two subgenomes ofC. songoricaprovide equal contribution under HS on the basis of the distribution and expression of differentially expressed genes (DEGs). Furthermore, 216 DEGs were identified as key evolutionarily conserved genes involved in the response to HS inC. songoricavia comparative analysis with genes of four Poaceae species; these genes were involved in the 'response to heat' and 'heat acclimation'. Notably, most of the conserved DEGs belonged to the heat-shock protein (HSP) superfamily. Similar results were also obtained from co-expression analysis. Interestingly, hub-genes of co-expression analysis were found to overlap with conserved genes, especially heat-shock protein (HSP). InC. songorica, 84HSPand 32 heat-shock transcription factor (HSF) genes were identified in the allotetraploidC. songoricagenome, and might have undergone purifying selection during evolutionary history based on syntenic and phylogenetic analysis. By analysing the expression patterns of theCsHSPs andCsHSFs, we found that the transcript abundance of 72.7% of theCsHSPgenes and of 62.5% of theCsHSFgenes changed under heat stress in both the shoots and roots. Finally, a core regulatory network of HS was constructed on the basis of theCsHSP,CsHSFand other responsive genes inC. songorica. Conclusions Regulatory network and key genes were comprehensively analysed and identified inC. songoricaunder HS. This study improves our knowledge of thermotolerance mechanisms in native grasses, and also provides candidate genes for potential applications in the genetic improvement of grasses.
英文关键词	Heat stress Cleistogenes songorica RNA sequencing Conserved heat-responsive genes Co-expression Regulatory network HSP HSF
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000582738500001
WOS关键词	TRANSCRIPTION FACTOR HSFA3 ; CLEISTOGENES-SONGORICA ; ARABIDOPSIS-THALIANA ; DROUGHT TOLERANCE ; PHOSPHOLIPASE-D ; SHOCK PROTEINS ; SALT STRESS ; EXPRESSION ; IDENTIFICATION ; DREB2A
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
来源机构	兰州大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/327156
作者单位	[Yan, Qi; Zong, Xifang; Wu, Fan; Li, Jie; Ma, Tiantian; Zhao, Yufeng; Ma, Qian; Wang, Penglei; Wang, Yanrong; Zhang, Jiyu] Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Engn Res Ctr Grassland Ind,State Key Lab Grasslan, Minist Educ,Key Lab Grassland Livestock Ind Innov, Lanzhou 730020, Peoples R China
推荐引用方式 GB/T 7714	Yan, Qi,Zong, Xifang,Wu, Fan,et al. Integrated analysis of co-expression, conserved genes and gene families reveal core regulatory network of heat stress response inCleistogenes songorica, a xerophyte perennial desert plant[J]. 兰州大学,2020,21(1).
APA	Yan, Qi.,Zong, Xifang.,Wu, Fan.,Li, Jie.,Ma, Tiantian.,...&Zhang, Jiyu.(2020).Integrated analysis of co-expression, conserved genes and gene families reveal core regulatory network of heat stress response inCleistogenes songorica, a xerophyte perennial desert plant.BMC GENOMICS,21(1).
MLA	Yan, Qi,et al."Integrated analysis of co-expression, conserved genes and gene families reveal core regulatory network of heat stress response inCleistogenes songorica, a xerophyte perennial desert plant".BMC GENOMICS 21.1(2020).