干旱区生态环境知识资源中心(Arid): Epithelial WNT2B and Desert Hedgehog Are Necessary for Human Colonoid Regeneration after Bacterial Cytotoxin Injury

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DOI	10.1016/j.isci.2020.101618
	Epithelial WNT2B and Desert Hedgehog Are Necessary for Human Colonoid Regeneration after Bacterial Cytotoxin Injury
	In, Julie G.; Yin, Jianyi; Atanga, Roger; Doucet, Michele; Cole, Robert N.; DeVine, Lauren; Donowitz, Mark; Zachos, Nicholas C.; Blutt, Sarah E.; Estes, Mary K.; Kovbasnjuk, Olga
通讯作者	In, JG
来源期刊	ISCIENCE
EISSN	2589-0042
出版年	2020
卷号	23 期号:10
英文摘要	Intestinal regeneration and crypt hyperplasia after radiation or pathogen injury relies on Wnt signaling to stimulate stem cell proliferation. Mesenchymal Wnts are essential for homeostasis and regeneration in mice, but the role of epithelial Wnts remains largely uncharacterized. Using the enterohemorrhagic E. coli-secreted cytotoxin EspP to induce injury to human colonoids, we evaluated a simplified, epithelial regeneration model that lacks mesenchymal Wnts. Here, we demonstrate that epithelial-produced WNT2B is upregulated following injury and essential for regeneration. Hedgehog signaling, specifically activation via the ligand Desert Hedgehog (DHH), but not Indian or Sonic Hedgehog, is another driver of regeneration and modulates WNT2B expression. These findings highlight the importance of epithelial WNT2B and DHH in regulating human colonic regeneration after injury.
类型	Article
语种	英语
开放获取类型	Green Submitted, gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000581985500073
WOS关键词	INTESTINAL STEM-CELLS ; INDIAN HEDGEHOG ; WNT/BETA-CATENIN ; SIGNALING PATHWAYS ; HOMEOSTASIS ; EXPRESSION ; PROLIFERATION ; BIOGENESIS ; BINDING ; ADENOMA
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/327101
作者单位	[In, Julie G.; Atanga, Roger; Kovbasnjuk, Olga] Univ New Mexico, Dept Internal Med, Div Gastroenterol & Hepatol, Albuquerque, NM 87131 USA; [In, Julie G.; Yin, Jianyi; Doucet, Michele; Donowitz, Mark; Zachos, Nicholas C.; Kovbasnjuk, Olga] Johns Hopkins Univ, Sch Med, Dept Med, Div Gastroenterol & Hepatol, Baltimore, MD 21205 USA; [Cole, Robert N.; DeVine, Lauren] Johns Hopkins Univ, Sch Med, Dept Biol Chem, Mass Spectrometry & Prote Facil, Baltimore, MD 21205 USA; [Blutt, Sarah E.; Estes, Mary K.] Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA; [Estes, Mary K.] Baylor Coll Med, Dept Med, Sect Gastroenterol & Hepatol, Houston, TX 77030 USA
推荐引用方式 GB/T 7714	In, Julie G.,Yin, Jianyi,Atanga, Roger,et al. Epithelial WNT2B and Desert Hedgehog Are Necessary for Human Colonoid Regeneration after Bacterial Cytotoxin Injury[J],2020,23(10).
APA	In, Julie G..,Yin, Jianyi.,Atanga, Roger.,Doucet, Michele.,Cole, Robert N..,...&Kovbasnjuk, Olga.(2020).Epithelial WNT2B and Desert Hedgehog Are Necessary for Human Colonoid Regeneration after Bacterial Cytotoxin Injury.ISCIENCE,23(10).
MLA	In, Julie G.,et al."Epithelial WNT2B and Desert Hedgehog Are Necessary for Human Colonoid Regeneration after Bacterial Cytotoxin Injury".ISCIENCE 23.10(2020).