干旱区生态环境知识资源中心(Arid): Fully Automated Microchip Electrophoresis Analyzer for Potential Life Detection Missions

Arid

DOI	10.1021/acs.analchem.0c01628
	Fully Automated Microchip Electrophoresis Analyzer for Potential Life Detection Missions
	Mora, Maria F.; Kehl, Florian; Costa, Eric Tavares; Bramall, Nathan; Willis, Peter A.
通讯作者	Willis, PA
来源期刊	ANALYTICAL CHEMISTRY
ISSN	0003-2700
EISSN	1520-6882
出版年	2020
卷号	92 期号:19 页码:12959-12966
英文摘要	There are a variety of complementary observations that could be used in the search for life in extraterrestrial settings. At the molecular scale, patterns in the distribution of organics could provide powerful evidence of a biotic component. In order to observe these molecular biosignatures during spaceflight missions, it is necessary to perform separation science in situ. Microchip electrophoresis (ME) is ideally suited for this task. Although this technique is readily miniaturized and numerous instruments have been developed over the last 3 decades, to date, all lack the automation capabilities needed for future missions of exploration. We have developed a portable, automated, battery-powered, and remotely operated ME instrument coupled to laser-induced fluorescence detection. This system contains all the necessary hardware and software interfaces for end-to-end functionality. Here, we report the first application of the system for amino acid analysis coupled to an extraction unit in order to demonstrate automated sample-to-data operation. The system was remotely operated aboard a rover during a simulated Mars mission in the Atacama Desert, Chile. This is the first demonstration of a fully automated ME analysis of soil samples relevant to planetary exploration. This validation is a critical milestone in the advancement of this technology for future implementation on a spaceflight mission.
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000580426800029
WOS关键词	CAPILLARY-ELECTROPHORESIS ; MEMBRANE VALVES ; PUMPS ; INSTRUMENTATION ; SEPARATIONS ; MICRODEVICE ; SEARCH
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/326972
作者单位	[Mora, Maria F.; Kehl, Florian; Costa, Eric Tavares; Willis, Peter A.] CALTECH, Jet Prop Lab, Pasadena, CA 91109 USA; [Bramall, Nathan] Leiden Measurement Technol LLC, Sunnyvale, CA 94089 USA
推荐引用方式 GB/T 7714	Mora, Maria F.,Kehl, Florian,Costa, Eric Tavares,et al. Fully Automated Microchip Electrophoresis Analyzer for Potential Life Detection Missions[J],2020,92(19):12959-12966.
APA	Mora, Maria F.,Kehl, Florian,Costa, Eric Tavares,Bramall, Nathan,&Willis, Peter A..(2020).Fully Automated Microchip Electrophoresis Analyzer for Potential Life Detection Missions.ANALYTICAL CHEMISTRY,92(19),12959-12966.
MLA	Mora, Maria F.,et al."Fully Automated Microchip Electrophoresis Analyzer for Potential Life Detection Missions".ANALYTICAL CHEMISTRY 92.19(2020):12959-12966.