干旱区生态环境知识资源中心(Arid): General Microbiota of the Soft TickOrnithodoros turicataParasitizing the Bolson Tortoise (Gopherus flavomarginatus) in the Mapimi Biosphere Reserve, Mexico

Arid

DOI	10.3390/biology9090275
	General Microbiota of the Soft TickOrnithodoros turicataParasitizing the Bolson Tortoise (Gopherus flavomarginatus) in the Mapimi Biosphere Reserve, Mexico
	Barraza-Guerrero, Sergio I.; Meza-Herrera, Cesar A.; de la Pena, Cristina; Gonzalez-Alvarez, Vicente H.; Vaca-Paniagua, Felipe; Diaz-Velasquez, Clara E.; Sanchez-Tortosa, Francisco; Avila-Rodriguez, Veronica; Valenzuela-Nunez, Luis M.; Herrera-Salazar, Juan C.
通讯作者	de la Pena, CG
来源期刊	BIOLOGY-BASEL
EISSN	2079-7737
出版年	2020
卷号	9 期号:9
英文摘要	The general bacterial microbiota of the soft tickOrnithodoros turicatafound on Bolson tortoises (Gopherus flavomarginatus) were analyzed using next generation sequencing. The main aims of the study were to establish the relative abundance of bacterial taxa in the tick, and to document the presence of potentially pathogenic species for this tortoise, other animals, and humans. The study was carried-out in the Mapimi Biosphere Reserve in the northern-arid part of Mexico. Bolson tortoises (n= 45) were inspected for the presence of soft ticks, from which 11 tortoises (24.4%) had ticks in low loads (1-3 ticks per individual). Tick pools (five adult ticks each) were analyzed through 16S rRNA V3-V4 region amplification in a MiSeq Illumina, using EzBioCloud as a taxonomical reference. The operational taxonomic units (OTUs) revealed 28 phyla, 84 classes, 165 orders, 342 families, 1013 genera, and 1326 species. The high number of taxa registered forO. turicatamay be the result of the variety of hosts that this tick parasitizes as they live insideG. flavomarginatusburrows. While the most abundant phyla were Proteobacteria, Actinobacteria, and Firmicutes, the most abundant species were two endosymbionts of ticks (Midichloria-like andCoxiella-like). Two bacteria documented as pathogenic toGopherusspp. were registered (Mycoplasmaspp. andPasteurella testudinis). The bovine and ovine tick-borne pathogensA. marginaleandA. ovis, respectively, were recorded, as well as the zoonotic bacteriaA. phagocytophilum,Coxiella burnetii, andNeoehrlichiasp. Tortoises parasitized withO. turicatadid not show evident signs of disease, which could indicate a possible ecological role as a reservoir that has yet to be demonstrated. In fact, the defense mechanisms of this tortoise against the microorganisms transmitted by ticks during their feeding process are still unknown. Future studies on soft ticks should expand our knowledge about what components of the microbiota are notable across multiple host-microbe dynamics. Likewise, studies are required to better understand the host competence of this tortoise, considered the largest terrestrial reptile in North America distributed throughout the Chihuahuan Desert since the late Pleistocene.
英文关键词	microbiota soft ticks Bolson tortoises bacterial profile tick-borne pathogens
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000580272300001
WOS关键词	Q-FEVER ; MIDICHLORIA-MITOCHONDRII ; FAMILY ANAPLASMATACEAE ; IXODES-RICINUS ; TICK ; TRANSMISSION ; TOLERANCE ; INFECTION ; COXIELLA ; ACARI
WOS类目	Biology
WOS研究方向	Life Sciences & Biomedicine - Other Topics
来源机构	Universidad Nacional Autónoma de México
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/326949
作者单位	[Barraza-Guerrero, Sergio I.; Meza-Herrera, Cesar A.] Univ Autonoma Chapingo, Unidad Reg Univ Zonas Aridas, Durango 35230, Mexico; [de la Pena, Cristina; Avila-Rodriguez, Veronica; Valenzuela-Nunez, Luis M.; Herrera-Salazar, Juan C.] Univ Juarez Estado Durango, Fac Ciencias Biol, Durango 35010, Mexico; [Gonzalez-Alvarez, Vicente H.] Univ Autonoma Guerrero, Fac Med Vet & Zootecnia 2, Cuajinicuilapa 41940, Guerrero, Mexico; [Vaca-Paniagua, Felipe; Diaz-Velasquez, Clara E.] Fac Estudios Superiores Iztacala, Lab Nacl Salud Diagnost Mol & Efecto Ambiental En, Tlalnepantla 54090, Estado De Mexic, Mexico; [Vaca-Paniagua, Felipe] Inst Nacl Cancerol, Mexico City 14080, DF, Mexico; [Vaca-Paniagua, Felipe] Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Tlalnepantla 54090, Estado De Mexic, Mexico; [Sanchez-Tortosa, Francisco] Univ Cordoba, Dept Zool, Edificio C-1,Campus Rabanales, Cordoba 14071, Spain
推荐引用方式 GB/T 7714	Barraza-Guerrero, Sergio I.,Meza-Herrera, Cesar A.,de la Pena, Cristina,et al. General Microbiota of the Soft TickOrnithodoros turicataParasitizing the Bolson Tortoise (Gopherus flavomarginatus) in the Mapimi Biosphere Reserve, Mexico[J]. Universidad Nacional Autónoma de México,2020,9(9).
APA	Barraza-Guerrero, Sergio I..,Meza-Herrera, Cesar A..,de la Pena, Cristina.,Gonzalez-Alvarez, Vicente H..,Vaca-Paniagua, Felipe.,...&Herrera-Salazar, Juan C..(2020).General Microbiota of the Soft TickOrnithodoros turicataParasitizing the Bolson Tortoise (Gopherus flavomarginatus) in the Mapimi Biosphere Reserve, Mexico.BIOLOGY-BASEL,9(9).
MLA	Barraza-Guerrero, Sergio I.,et al."General Microbiota of the Soft TickOrnithodoros turicataParasitizing the Bolson Tortoise (Gopherus flavomarginatus) in the Mapimi Biosphere Reserve, Mexico".BIOLOGY-BASEL 9.9(2020).