干旱区生态环境知识资源中心(Arid): Short communication: Locus-specific interrelations between gene expression and DNA methylation patterns in bovine mammary gland infected by coagulase-positive and coagulase-negative staphylococci

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DOI	10.3168/jds.2020-18404
	Short communication: Locus-specific interrelations between gene expression and DNA methylation patterns in bovine mammary gland infected by coagulase-positive and coagulase-negative staphylococci
	Zabek, T.; Semik-Gurgul, E.; Ropka-Molik, K.; Szmatola, T.; Kawecka-Grochocka, E.; Zalewska, M.; Kosciuczuk, E.; Wnuk, M.; Bagnicka, E.
通讯作者	Zabek, T
来源期刊	JOURNAL OF DAIRY SCIENCE
ISSN	0022-0302
EISSN	1525-3198
出版年	2020
卷号	103 期号:11 页码:10689-10695
英文摘要	Pathogens are able to alter the cell cycle program and immune response of the host by changing the transcription and epigenetics of genes responsible for cell cycle control and inflammation. In this regard, we evaluated interrelations between DNA methylation and expression of autophagy, apoptosis, and lipid metabolism-related genes in a sample set of mammary gland secretory tissue sections derived from bovine mammary glands infected with coagulase-negative and coagulase-positive staphylococci. We assessed relative transcript abundance and DNA bisulfite sequencing in loci of the ATG5, IGF1R, TERT, and DGAT1 genes. Lack of DNA methylation in ATG5 arid DGAT1 loci might be associated with maintenance of ATG5 and DGAT1 expression regardless of the health status of bovine mammary gland. Complete inethylation of intragenic CpG regions in the IGF1R locus was apparently riot related to the presence of its transcript in the investigated udder parenchyma samples. Detected hypermethylation of the TERT upstream element was associated with a small amount of TERT niRNA in bovine mammary gland, regardless of the presence, or absence, of the pathogen. A significant decrease in TERT gene expression in tissue sections of mammary gland free of bacteria and in those infected with coagulase-positive staphylococci was observed in parenchyma samples infected with coagulase-negative staphylococci. Two possible explanations are the direct involvement of the TERT gene in the etiology of bovine mastitis or the increase of TERT niRNA due to activation of the MAPK signaling pathway in response to release of exotoxins by coagulase-negative bacteria in the bovine mammary gland.
英文关键词	mastitis DNA methylation differential gene expression
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000579895400084
WOS关键词	TELOMERASE ; MECHANISMS ; CELLS
WOS类目	Agriculture, Dairy & Animal Science ; Food Science & Technology
WOS研究方向	Agriculture ; Food Science & Technology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/326916
作者单位	[Zabek, T.; Semik-Gurgul, E.; Ropka-Molik, K.; Szmatola, T.] Natl Res Inst Anim Prod, Krakowska 1, PL-32083 Balice, Poland; [Szmatola, T.] Agr Univ Krakow, Univ Ctr Vet Med Krakow, Al Mickiewicza 24-28, PL-30059 Krakow, Poland; [Kawecka-Grochocka, E.; Zalewska, M.; Kosciuczuk, E.; Bagnicka, E.] Polish Acad Sci, Inst Genet & Anim Breeding, Postepu 36A, PL-05552 Magdalenka, Poland; [Zalewska, M.] Univ Warsaw, Fac Biol, Inst Microbiol, Dept Appl Microbiol, Miecznikowa 1, PL-02096 Warsaw, Poland; [Wnuk, M.] Univ Rzeszow, Dept Biotechnol, Pigonia 1, PL-35310 Rzeszow, Poland
推荐引用方式 GB/T 7714	Zabek, T.,Semik-Gurgul, E.,Ropka-Molik, K.,et al. Short communication: Locus-specific interrelations between gene expression and DNA methylation patterns in bovine mammary gland infected by coagulase-positive and coagulase-negative staphylococci[J],2020,103(11):10689-10695.
APA	Zabek, T..,Semik-Gurgul, E..,Ropka-Molik, K..,Szmatola, T..,Kawecka-Grochocka, E..,...&Bagnicka, E..(2020).Short communication: Locus-specific interrelations between gene expression and DNA methylation patterns in bovine mammary gland infected by coagulase-positive and coagulase-negative staphylococci.JOURNAL OF DAIRY SCIENCE,103(11),10689-10695.
MLA	Zabek, T.,et al."Short communication: Locus-specific interrelations between gene expression and DNA methylation patterns in bovine mammary gland infected by coagulase-positive and coagulase-negative staphylococci".JOURNAL OF DAIRY SCIENCE 103.11(2020):10689-10695.