干旱区生态环境知识资源中心(Arid): Exposure to low Earth orbit of an extreme-tolerant cyanobacterium as a contribution to lunar astrobiology activities

Arid

DOI	10.1017/S1473550419000168
	Exposure to low Earth orbit of an extreme-tolerant cyanobacterium as a contribution to lunar astrobiology activities
	Billi, Daniela; Mosca, Claudia; Fagliarone, Claudia; Napoli, Alessandro; Verseux, Cyprien; Baqu, Mickael; De Vera, Jean-Pierre
通讯作者	Billi, D
来源期刊	INTERNATIONAL JOURNAL OF ASTROBIOLOGY
ISSN	1473-5504
EISSN	1475-3006
出版年	2020
卷号	19 期号:1 页码:53-60
英文摘要	By investigating the survival and the biomarker detectability of a rock-inhabiting cyanobacterium,Chroococcidiopsissp. CCMEE 029, the BIOMEX space experiment might contribute to a future exploitation of the Moon as a test-bed for key astrobiology tasks such as the testing of life-detection technologies and the study of life in space. Post-flight analyses demonstrated that the mixing of dried cells with sandstone and a lunar regolith simulant provided protection against space UV radiation. During the space exposure, dried cells not mixed with minerals were killed by 2.05 x 10(2)kJ m(-2)of UV radiation, while cells mixed with sandstone or lunar regolith survived 1.59 x 10(2)and 1.79 x 10(2)kJ m(-2), respectively. No differences in survival occurred among cells mixed and not mixed with minerals and exposed to space conditions in the dark; this finding suggests that space vacuum and 0.5 Gy of ionizing radiation did not impair the cells' presence in space. The genomic DNA of dead cells was severely damaged but still detectable with PCR amplification of a short target, thus suggesting that short sequences should be targeted in a PCR-based approach when searching for traces of life. The enhanced stability of genomic DNA of dried cells mixed with minerals and exposed to space indicates that DNA might still be detectable after prolonged periods, possibly up to millions of years in microbes shielded by minerals. Overall, the BIOMEX results contribute to future experiments regarding the exposure of cells and their biomarkers to deep space conditions in order to further test the lithopanspermia hypothesis, the biomarker stability and the microbial endurance, with implications for planetary protection and to determine if the Moon has been contaminated during past human missions.
英文关键词	Biomarkers BIOMEX cyanobacteria ISS low Earth orbit Lunar astrobiology
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000578340300006
WOS关键词	DESERT CYANOBACTERIUM ; RADIATION-RESISTANCE ; BACTERIAL-SPORES ; MARS ; SPACE ; CHROOCOCCIDIOPSIS ; HABITABILITY ; DESICCATION ; LIFE ; DNA
WOS类目	Astronomy & Astrophysics ; Biology ; Geosciences, Multidisciplinary
WOS研究方向	Astronomy & Astrophysics ; Life Sciences & Biomedicine - Other Topics ; Geology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/326788
作者单位	[Billi, Daniela; Mosca, Claudia; Fagliarone, Claudia; Napoli, Alessandro] Univ Roma Tor Vergata, Dept Biol, Rome, Italy; [Verseux, Cyprien] Univ Bremen, Ctr Appl Space Technol & Micrograv, Bremen, Germany; [Baqu, Mickael; De Vera, Jean-Pierre] German Aerosp Ctr, Inst Planetary Res Management & Infrastruct, Astrobiol Labs, Berlin, Germany
推荐引用方式 GB/T 7714	Billi, Daniela,Mosca, Claudia,Fagliarone, Claudia,et al. Exposure to low Earth orbit of an extreme-tolerant cyanobacterium as a contribution to lunar astrobiology activities[J],2020,19(1):53-60.
APA	Billi, Daniela.,Mosca, Claudia.,Fagliarone, Claudia.,Napoli, Alessandro.,Verseux, Cyprien.,...&De Vera, Jean-Pierre.(2020).Exposure to low Earth orbit of an extreme-tolerant cyanobacterium as a contribution to lunar astrobiology activities.INTERNATIONAL JOURNAL OF ASTROBIOLOGY,19(1),53-60.
MLA	Billi, Daniela,et al."Exposure to low Earth orbit of an extreme-tolerant cyanobacterium as a contribution to lunar astrobiology activities".INTERNATIONAL JOURNAL OF ASTROBIOLOGY 19.1(2020):53-60.