干旱区生态环境知识资源中心(Arid): Identification of valid reference genes for quantitative RT-PCR inCaragana microphyllaunder salt and drought stresses

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DOI	10.1007/s12298-020-00874-3
	Identification of valid reference genes for quantitative RT-PCR inCaragana microphyllaunder salt and drought stresses
	Kim, Su Jung; Na, Jung Up; Kim, Ji Seong; Lee, Jeong Eun; Nie, Haulin; Lee, Keum Ah; Woo, Su Young; Kim, Sun Hyung
通讯作者	Kim, SH
来源期刊	PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
ISSN	0971-5894
EISSN	0974-0430
出版年	2020
卷号	26 期号:10 页码:2103-2108
英文摘要	Caragana microphyllais a leguminosae plant and grows mainly in semi-arid areas of northwest China and Mongolia. However, the lack of studies onC. microphyllareference genes limits the accurate understanding of the molecular biology mechanisms in this crop under abiotic stresses. In this study, we selected nine candidate genes from salt-treatedC. microphyllatranscriptome data and evaluated their stability by using geNorm, NormFinder, BestKeeper, and RefFinder in salt and drought conditions. In addition, the relative expressions of Delta 1-pyrroline-5-carboxylate synthase 2 (P5CS2) and Catalase 2 (CAT2) were examined to confirm the stability of the candidate reference genes. As a results, glyceraldehyde-3-phosphate dehydrogenase C2 (GAPC2) and 26S proteasome regulatory subunit (RPN5) were the most stable in both salt and drought treatments. The relative expression ofP5CS2andCAT2also showed more stable levels in normalization byGAPC2andRPN5than the most unstable gene,Ubiquitin 4(UBQ4). Therefore, it is believed that these candidate reference genes selected and validated in our study could be used to study the molecular biological study of response to salt and drought stress inC. microphylla.
英文关键词	Reference genes Gene expression Caragana microphylla qRT-PCR Salt stress Drought stress
类型	Article
语种	英语
开放获取类型	Green Published
收录类别	SCI-E
WOS记录号	WOS:000576124100001
WOS关键词	NORMALIZATION ; TOLERANCE ; KOM.
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/326669
作者单位	[Kim, Su Jung; Na, Jung Up; Kim, Ji Seong; Lee, Jeong Eun; Nie, Haulin; Lee, Keum Ah; Woo, Su Young; Kim, Sun Hyung] Univ Seoul, Dept Environm Hort, Seoul 02504, South Korea
推荐引用方式 GB/T 7714	Kim, Su Jung,Na, Jung Up,Kim, Ji Seong,et al. Identification of valid reference genes for quantitative RT-PCR inCaragana microphyllaunder salt and drought stresses[J],2020,26(10):2103-2108.
APA	Kim, Su Jung.,Na, Jung Up.,Kim, Ji Seong.,Lee, Jeong Eun.,Nie, Haulin.,...&Kim, Sun Hyung.(2020).Identification of valid reference genes for quantitative RT-PCR inCaragana microphyllaunder salt and drought stresses.PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS,26(10),2103-2108.
MLA	Kim, Su Jung,et al."Identification of valid reference genes for quantitative RT-PCR inCaragana microphyllaunder salt and drought stresses".PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS 26.10(2020):2103-2108.