干旱区生态环境知识资源中心(Arid): Genetic barcoding of Ecuadorian epilithic diatom species suitable as water quality bioindicators

Arid

DOI	10.5802/crbiol.2
	Genetic barcoding of Ecuadorian epilithic diatom species suitable as water quality bioindicators
	Ballesteros, Isabel; Castillejo, Pablo; Paulina Haro, Adriana; Cristina Montes, Cuthy; Heinrich, Carla; Lobo, Eduardo Alexis
通讯作者	Castillejo, P
来源期刊	COMPTES RENDUS BIOLOGIES
ISSN	1631-0691
EISSN	1768-3238
出版年	2020
卷号	343 期号:1 页码:41-52
英文摘要	Diatom identification is a key step in using these microorganisms as water quality bioindicators. Morphological diagnosis is a difficult task due to the enormous number of species and their microscopic size. This can be overcome using molecular tools to complement the diagnosis. The main goal of this work was to obtain the DNA barcode of Ecuadorian epilithic diatoms with a wide geographical distribution, a well-defined ecological range arid characteristics that allow them to be reliable indicator species. Unialgal diatom cultures were obtained from environmental samples of Ecuadorian Andean streams. Morphological characterization of cultures was carried out under SEM microscopy. For molecular characterization, 185V4 and rbcL barcodes were sequenced from each strain and blasted against a GenBank database. A phylogenetic tree for each barcode was constructed using the ML method including sequences of strains of the studied species from different geographical locations. The results showed the following five species to be suitable as bioindicators and these were isolated. Sellaphora seminulum (strain JA01 b, c), Nitzschia fonticola (strain SP02a) and N. palea (strain CA01a) are tolerant to eutrophication; Eolimna minima (strain CH02a) is a mesotrophic water bioindicator, and Achnanthidium minutissimum (strain JA01a) is an oligotrophic water bioindicator. The comparison with the GenBank database of the barcoding regions supported the morphological identification. The barcoding sequences of the strains showed a high percentage of identity with the sequences reported in INSDC databases for the same species. The topology of the phylogenetic trees demonstrates that epilithic diatoms from Ecuador are closely related to those of same species isolated from other geographical regions. This study is a first attempt to establish a morphological and molecular taxonomic reference library for neotropical diatoms. This study demonstrates that it would be feasible to use the existing barcoding data for diatoms to develop molecular tools for the bioassessment of aquatic ecosystems in the Ecuadorian Andean region.
英文关键词	Bacillariophytae 18SV4 rRNA gene rbcL gene Barcode reference libraries Biomonitoring Ecuador Diatoms
类型	Article
语种	英语
开放获取类型	Bronze
收录类别	SCI-E
WOS记录号	WOS:000573960100008
WOS关键词	DIVERSITY ; DNA ; IDENTIFICATION ; DISTRIBUTIONS ; MORPHOLOGY ; PHYLOGENY ; TAXONOMY ; NUMBER ; RBCL
WOS类目	Biology
WOS研究方向	Life Sciences & Biomedicine - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/326577
作者单位	[Ballesteros, Isabel; Castillejo, Pablo; Paulina Haro, Adriana; Cristina Montes, Cuthy] Univ Las Amer, Fac Ingn & Ciencias Aplicadas, Grp Invest Biodiversidad Medioambiente & Salud BI, Quito, Ecuador; [Heinrich, Carla; Lobo, Eduardo Alexis] Univ Santa Cruz do Sul UNISC, Santa Cruz Do Sul, RS, Brazil
推荐引用方式 GB/T 7714	Ballesteros, Isabel,Castillejo, Pablo,Paulina Haro, Adriana,et al. Genetic barcoding of Ecuadorian epilithic diatom species suitable as water quality bioindicators[J],2020,343(1):41-52.
APA	Ballesteros, Isabel,Castillejo, Pablo,Paulina Haro, Adriana,Cristina Montes, Cuthy,Heinrich, Carla,&Lobo, Eduardo Alexis.(2020).Genetic barcoding of Ecuadorian epilithic diatom species suitable as water quality bioindicators.COMPTES RENDUS BIOLOGIES,343(1),41-52.
MLA	Ballesteros, Isabel,et al."Genetic barcoding of Ecuadorian epilithic diatom species suitable as water quality bioindicators".COMPTES RENDUS BIOLOGIES 343.1(2020):41-52.