干旱区生态环境知识资源中心(Arid): Determination of anabasine, anatabine, and nicotine biomarkers in wastewater by enhanced direct injection LC-MS/MS and evaluation of their in-sewer stability

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DOI	10.1016/j.scitotenv.2020.140551
	Determination of anabasine, anatabine, and nicotine biomarkers in wastewater by enhanced direct injection LC-MS/MS and evaluation of their in-sewer stability
	Zheng, Qiuda; Eaglesham, Geoff; Tscharke, Benjamin J.; O'Brien, Jake W.; Li, Jiaying; Thompson, Jack; Shimko, Katja M.; Reeks, Tim; Gerber, Cobus; Thomas, Kevin, V; Thai, Phong K.
通讯作者	Zheng, QD
来源期刊	SCIENCE OF THE TOTAL ENVIRONMENT
ISSN	0048-9697
EISSN	1879-1026
出版年	2020
卷号	743
英文摘要	Wastewater-based epidemiology (WBE) has been used to estimate tobacco use in the population. However, the increased use of nicotine replacement therapies and e-cigarettes contributes to the load of nicotine metabolites in wastewater, causing over-estimation of tobacco use if nicotine metabolites were used in WBE back-estimation. This study aims to develop a rapid method for determining the tobacco-specific biomarkers, anabasine and anatabine, in wastewater and to evaluate their in-sewer stability for better estimation of tobacco use by WBE. An enhanced direct injection LC-MS/MS was developed to quantify anabasine and anatabine as well as nicotine biomarkers (nicotine, cotinine and hydroxycotinine). The method was optimal when wastewater was filtered through 0.2 mu m RC syringe filters and a pre-conditioned SPE cartridge (Oasis HLB 1 cc, 30 mg) before 50 mu L was injected into the LC-MS/MS system. Limits of quantification varied between 2.7 and 54.9 ng/L with recoveries from 76% to 103% for all five compounds. In sewer reactors, anabasine and anatabine were less stable than cotinine and hydroxycotinine. They were more stable in the gravity sewer reactor with <20% loss in 12 h than in the rising main sewer reactor with similar to 30% loss in the same period. We then applied the new method to 42 daily wastewater influent samples collected from an Australian wastewater treatment plant. The five biomarkers were detected in all samples with concentrations ranging from 9.2 to 7430 ng/L. All five compounds were positively correlated with one another. Our results suggested a high throughput analytical method for feasible application in anabasine and anatabine as biomarkers of tobacco use in routine wastewater monitoring. (C) 2020 Elsevier B.V. All rights reserved.
英文关键词	Direct injection Nicotine biomarkers Anabasine Anatabine Nicotine
类型	Article
语种	英语
收录类别	SCI-E ; SSCI
WOS记录号	WOS:000573538400005
WOS关键词	TANDEM MASS-SPECTROMETRY ; MINOR TOBACCO ALKALOIDS ; LIQUID-CHROMATOGRAPHY ; ILLICIT DRUGS ; CONSUMPTION ; EPIDEMIOLOGY ; METABOLITES ; ALCOHOL ; TRENDS ; TRANSFORMATION
WOS类目	Environmental Sciences
WOS研究方向	Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/326546
作者单位	[Zheng, Qiuda; Eaglesham, Geoff; Tscharke, Benjamin J.; O'Brien, Jake W.; Thompson, Jack; Shimko, Katja M.; Reeks, Tim; Thomas, Kevin, V; Thai, Phong K.] Univ Queensland, Queensland Alliance Environm Hlth Sci QAEHS, 20 Cornwall St, Woolloongabba, Qld 4102, Australia; [Li, Jiaying] Univ Queensland, Adv Water Management Ctr, St Lucia, Qld 4072, Australia; [Gerber, Cobus] Univ South Australia, Clin & Hlth Sci, Adelaide, SA 5001, Australia
推荐引用方式 GB/T 7714	Zheng, Qiuda,Eaglesham, Geoff,Tscharke, Benjamin J.,et al. Determination of anabasine, anatabine, and nicotine biomarkers in wastewater by enhanced direct injection LC-MS/MS and evaluation of their in-sewer stability[J],2020,743.
APA	Zheng, Qiuda.,Eaglesham, Geoff.,Tscharke, Benjamin J..,O'Brien, Jake W..,Li, Jiaying.,...&Thai, Phong K..(2020).Determination of anabasine, anatabine, and nicotine biomarkers in wastewater by enhanced direct injection LC-MS/MS and evaluation of their in-sewer stability.SCIENCE OF THE TOTAL ENVIRONMENT,743.
MLA	Zheng, Qiuda,et al."Determination of anabasine, anatabine, and nicotine biomarkers in wastewater by enhanced direct injection LC-MS/MS and evaluation of their in-sewer stability".SCIENCE OF THE TOTAL ENVIRONMENT 743(2020).