干旱区生态环境知识资源中心(Arid): Development and validation of a liquid chromatography coupled to tandem mass spectrometry method for the simultaneous quantification of five analgesics and sedatives, and six of their active metabolites in human plasma: Application to a clinical study on the determination of neurological death in the intensive care unit

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DOI	10.1016/j.jpba.2020.113521
	Development and validation of a liquid chromatography coupled to tandem mass spectrometry method for the simultaneous quantification of five analgesics and sedatives, and six of their active metabolites in human plasma: Application to a clinical study on the determination of neurological death in the intensive care unit
	Jutras, Martin; Williamson, David; Chasse, Michael; Leclair, Gregoire
通讯作者	Leclair, G
来源期刊	JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
ISSN	0731-7085
EISSN	1873-264X
出版年	2020
卷号	190
英文摘要	A sensitive and selective high-performance liquid chromatographic method coupled to tandem mass spectrometry was developed and validated for the quantification of morphine, hydromorphone, fentanyl, midazolam and propofol and their metabolites morphine-3-beta-D glucuronide, morphine- 6-beta-D glucuronide, hydromorphone 3-beta-D glucuronide, 1'-hydroxymidazolam-beta-D glucuronide, alpha-hydroxymidazolam and 4-hydroxymidazolam in human plasma using potassium oxalate/sodium fluoride mixture as anticoagulant. Human plasma samples (0.4 mL) to which were added a mixture of eleven deuterated internal standards were subjected to solid phase extraction using a mixed-mode polymeric Oasis PRiME MCX in 96-well format. Propofol was selectively eluted and further derivatized using 2-Fluoro-1-methylpyridinium p-toluenesulfonate, whereas the remaining 10 analytes were eluted separately and further concentrated. The derivatized propofol was analyzed separately in a second injection. The analytes were chromatographically separated on a Kinetex phenyl-hexyl analytical column in gradient elution mode, using a mobile phase consisting of aqueous ammonium formate/formic acid buffer and methanol. The overall run time was 8 min. Detection was performed using an AB/SCIEX 4000 QTRAP instrument with positive electrospray ionization employing scheduled multiple reaction monitoring mode. The lower limits of quantification ranged from 0.02 to 5 ng/mL depending on the analyte. Calibration curves covered a concentration range of 1000x in all cases but 1'-hydroxymidazolam-beta-D-glucuronide where it covered a range of 500 x . The validated method was accurate and precise, the intra-day accuracy and precision of quality control samples (4 concentration levels, n = 6 each) being within 91.5-112 % and 1.3-13.2 % (coefficient of variation), respectively, and inter-day (n = 24; 4 days) accuracy and precision of quality control samples (3 concentration levels) being within 94.8-103.5 % and 3.2-11.2 % (coefficient of variation). Mean absolute extraction recoveries were above 60 % for all compounds, except for hydromorphone 3-beta-D-glucuronide (44 %) and for 1'-hydroxymidazolam-beta-D-glucuronide (33 %). Internal standard corrected matrix effect ranged from -4.8 to 3.8 % in normal plasma and in plasma containing 1 % hemolyzed blood. Analytes were stable (above 90 %) in plasma and blood for 19 hat 22 degrees C, in blood for 90 hat 5 degrees C, in plasma for 60 days at -20 degrees C, for 4 months at -70 degrees C and after three freeze-thaw cycles, and in the injection solvent for at least 3 days in the autosampler. The present method is successfully being applied in a multicenter clinical study for the analysis of plasma samples from patients in intensive care units from a number of Canadian hospitals. (C) 2020 Elsevier B.V. All rights reserved.
英文关键词	Method validation Liquid chromatography coupled to tandem mass spectrometry Human plasma Sedatives Analgesics Neurological death
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000573434900024
WOS关键词	SOLID-PHASE EXTRACTION ; PROPOFOL ; MIDAZOLAM ; MORPHINE ; FENTANYL ; ASSAY ; MORPHINE-6-BETA-GLUCURONIDE ; MORPHINE-3-BETA-GLUCURONIDE ; HYDROMORPHONE ; NORFENTANYL
WOS类目	Chemistry, Analytical ; Pharmacology & Pharmacy
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/326542
作者单位	[Jutras, Martin; Williamson, David; Leclair, Gregoire] Univ Montreal, Fac Pharm, Platform Biopharm, Pavillon Jean Coutu,2940 Chemin Polytech, Montreal, PQ H3T 1J4, Canada; [Williamson, David] Hop Sacre Coeur Montreal, Res Ctr, Montreal, PQ H4J 1C5, Canada; [Williamson, David] Hop Sacre Coeur Montreal, Pharm Dept, Montreal, PQ H4J 1C5, Canada; [Chasse, Michael] Univ Montreal, Fac Med, Montreal, PQ H3T 1J4, Canada; [Chasse, Michael] Ctr Hosp Univ Montreal, Res Ctr, Montreal, PQ H2X 0A9, Canada; [Chasse, Michael] Ctr Hosp Univ Montreal, Dept Med Crit Care, Montreal, PQ H2X 0A9, Canada
推荐引用方式 GB/T 7714	Jutras, Martin,Williamson, David,Chasse, Michael,et al. Development and validation of a liquid chromatography coupled to tandem mass spectrometry method for the simultaneous quantification of five analgesics and sedatives, and six of their active metabolites in human plasma: Application to a clinical study on the determination of neurological death in the intensive care unit[J],2020,190.
APA	Jutras, Martin,Williamson, David,Chasse, Michael,&Leclair, Gregoire.(2020).Development and validation of a liquid chromatography coupled to tandem mass spectrometry method for the simultaneous quantification of five analgesics and sedatives, and six of their active metabolites in human plasma: Application to a clinical study on the determination of neurological death in the intensive care unit.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,190.
MLA	Jutras, Martin,et al."Development and validation of a liquid chromatography coupled to tandem mass spectrometry method for the simultaneous quantification of five analgesics and sedatives, and six of their active metabolites in human plasma: Application to a clinical study on the determination of neurological death in the intensive care unit".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 190(2020).