干旱区生态环境知识资源中心(Arid): Rotating-disk sorptive extraction for the determination of sex hormones and triclosan in urine by gas chromatography-mass spectrometry: Clean-up integrated steps and improved derivatization

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DOI	10.1016/j.microc.2020.105149
	Rotating-disk sorptive extraction for the determination of sex hormones and triclosan in urine by gas chromatography-mass spectrometry: Clean-up integrated steps and improved derivatization
	Arismendi, Daniel; Diaz, Karolina; Aguilera-Maraboli, Natalie; Sepulveda, Betsabet; Richter, Pablo
通讯作者	Arismendi, D ; Richter, P
来源期刊	MICROCHEMICAL JOURNAL
ISSN	0026-265X
EISSN	1095-9149
出版年	2020
卷号	158
英文摘要	Different clean-up stages were coupled to rotating-disk sorptive extraction (RDSE) of testosterone, progesterone, 17 beta-estradiol and triclosan in urine samples prior to derivatization and detection by gas chromatography-mass spectrometry (GC-MS). By using Oasis (R) HLB as the sorptive phase, extraction equilibrium was reached after 60 min at a disk rotation velocity of 2000 rpm. The factors involved in sample preparation of the urine were comprehensively studied and implemented to minimize matrix effects that were mainly produced by polar pigments in the urine. A 10-fold dilution of the sample was necessary prior to RDSE, followed by a washing step of the sorptive phase with 10% (v/v) methanolic solution and final selective desorption of the analytes with ethyl acetate. Derivatization of the analytes was also studied in detail and implemented prior to GC-MS. The reaction was optimized in terms of derivatizing agent consumption, time and temperature, achieving significant improvements in these factors. Under the optimized conditions, the matrix effects decreased almost five-fold for all analytes, and the relative recoveries were between 89 and 111% with detection limits in the range of 0.004-0.54 ng mL(-1), whereas the precision, expressed as relative standard deviation (RSD), was below 14%. Analytes were determined in real samples in the presence and absence of enzymatic hydrolysis, assessing both their free and total forms. The free triclosan concentration was only 10% of the total concentration found in the same sample after hydrolysis. Estradiol and testosterone were quantified with high sensitivity at concentrations between 0.11 and 10.45 and 0.20-21.23 ng mL(-1), respectively. Progesterone was only quantified in a urine sample from a woman during pregnancy.
英文关键词	Rotating-disk sorptive extraction (RDSE) Clean-up Urine Derivatization Sex hormones Triclosan
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000573291500003
WOS关键词	LIQUID-CHROMATOGRAPHY ; PROSTATE-CANCER ; WATER ; TESTOSTERONE ; PLASMA ; SERUM ; CHEMICALS ; ESTROGENS ; STEROIDS ; SAMPLES
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/326537
作者单位	[Arismendi, Daniel; Diaz, Karolina; Aguilera-Maraboli, Natalie; Sepulveda, Betsabet; Richter, Pablo] Univ Chile, Fac Chem & Pharmaceut Sci, Dept Inorgan & Analyt Chem, POB 233, Santiago, Chile
推荐引用方式 GB/T 7714	Arismendi, Daniel,Diaz, Karolina,Aguilera-Maraboli, Natalie,et al. Rotating-disk sorptive extraction for the determination of sex hormones and triclosan in urine by gas chromatography-mass spectrometry: Clean-up integrated steps and improved derivatization[J],2020,158.
APA	Arismendi, Daniel,Diaz, Karolina,Aguilera-Maraboli, Natalie,Sepulveda, Betsabet,&Richter, Pablo.(2020).Rotating-disk sorptive extraction for the determination of sex hormones and triclosan in urine by gas chromatography-mass spectrometry: Clean-up integrated steps and improved derivatization.MICROCHEMICAL JOURNAL,158.
MLA	Arismendi, Daniel,et al."Rotating-disk sorptive extraction for the determination of sex hormones and triclosan in urine by gas chromatography-mass spectrometry: Clean-up integrated steps and improved derivatization".MICROCHEMICAL JOURNAL 158(2020).