干旱区生态环境知识资源中心(Arid): Simultaneous extraction and determination of 45 veterinary antibiotics in swine manure by liquid chromatography-tandem mass spectrometry

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DOI	10.1016/j.jchromb.2020.122286
	Simultaneous extraction and determination of 45 veterinary antibiotics in swine manure by liquid chromatography-tandem mass spectrometry
	Zhi, Suli; Zhou, Jing; Liu, Haixue; Wu, Huihui; Zhang, Zulin; Ding, Yongzhen; Zhang, Keqiang
通讯作者	Zhang, KQ
来源期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN	1570-0232
EISSN	1873-376X
出版年	2020
卷号	1154
英文摘要	A multiresidue method for identification and quantification of 45 veterinary antibiotics (VAs) belonging to 5 different drug classes has been developed, which is the first comprehensive method for pig manure. The proposed method used mixed liquor with methanol: acetonitrile: citrate buffer ratio of 1:1:2 to extract the target VAs from freeze-dried manure. The extracting solution passed through solid-phase extraction (SPE) procedure with Oasis HLB cartridges, which could isolate the target VAs and clean the samples in a single step. After blow-drying and concentrating, the samples were analyzed by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) in one single injection with a run time of 12 min. The average recovery rates for most of the VAs were in the range of 50-120% with relative standard deviations below 15%. Linearity was studied in the range between 2 and 1000 mu g/kg using matrix-matched calibration and pure solvent calibration, to evaluate the matrix effects (MEs). The limits of detection (LDs) and quantification (LQs) were in the range of 0.01-1.54 mu g/kg and 0.03-5.13 mu g/kg respectively, except for penicillin G. Finally, the proposed method was used to quantify VA residues in real swine manure from three farms with different scales; tetracyclines, sulfonamides, quinolones and macrolides were the most frequently detected compounds.
英文关键词	Ultra-high-performance liquid chromatography Solid-phase extraction Extraction Veterinary antibiotics Swine manure Validation
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000572832000010
WOS关键词	SOLID-PHASE EXTRACTION ; WASTE-WATER ; RESISTANCE GENES ; PESTICIDE-RESIDUES ; EXCRETION MASSES ; TYPICAL SWINE ; DRUGS ; TETRACYCLINES ; SULFONAMIDES ; COMPOST
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/326507
作者单位	[Zhi, Suli; Ding, Yongzhen; Zhang, Keqiang] Minist Agr & Rural Affairs, Agroenvironm Protect Inst, Tianjin 300191, Peoples R China; [Zhou, Jing] Northeast Agr Univ, Coll Resources & Environm, Haerbin 150036, Peoples R China; [Liu, Haixue; Wu, Huihui] Tianjin Agr Univ, Agr Anal & Test Ctr, Tianjin 300384, Peoples R China; [Zhang, Zulin] James Hutton Inst, Aberdeen AB15 8QH, Scotland
推荐引用方式 GB/T 7714	Zhi, Suli,Zhou, Jing,Liu, Haixue,et al. Simultaneous extraction and determination of 45 veterinary antibiotics in swine manure by liquid chromatography-tandem mass spectrometry[J],2020,1154.
APA	Zhi, Suli.,Zhou, Jing.,Liu, Haixue.,Wu, Huihui.,Zhang, Zulin.,...&Zhang, Keqiang.(2020).Simultaneous extraction and determination of 45 veterinary antibiotics in swine manure by liquid chromatography-tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1154.
MLA	Zhi, Suli,et al."Simultaneous extraction and determination of 45 veterinary antibiotics in swine manure by liquid chromatography-tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1154(2020).