干旱区生态环境知识资源中心(Arid): Identification of a double-stranded RNA-degrading nuclease influencing both ingestion and injection RNA interference efficiency in the red flour beetle Tribolium castaneum

Arid

DOI	10.1016/j.ibmb.2020.103440
	Identification of a double-stranded RNA-degrading nuclease influencing both ingestion and injection RNA interference efficiency in the red flour beetle Tribolium castaneum
	Peng, Yingchuan; Wang, Kangxu; Chen, Jiasheng; Wang, Jinda; Zhang, Hainan; Ze, Longji; Zhu, Guanheng; Zhao, Chunqing; Xiao, Haijun; Han, Zhaojun
通讯作者	Han, ZJ
来源期刊	INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
ISSN	0965-1748
EISSN	1879-0240
出版年	2020
卷号	125
英文摘要	RNA interference (RNAi) efficiency dramatically varies among different insects and among administration methods. Numerous studies have revealed that a poor RNAi response is usually associated with a high double-stranded RNA (dsRNA)-degrading activity. Using the red flour beetle Tribolium castaneum, we conducted genome-wide identification of genes encoding dsRNA-degrading nucleases of the DNA/RNA non-specific endonuclease superfamily. To achieve a robust RNAi response in T. castaneum, four dsRNase genes were identified in the genome that seemed to be the potential factors reducing RNAi efficacy. Analysis of biochemical properties revealed that optimal conditions for the dsRNA-degrading activity were alkaline (pH 8.0) in the absence of Mg2+ at 37 degrees C. The dsRNA-degrading activity was predominantly present in the gut, and via hetemlogous expression and RNAi experimentation, gut-specific TcdsRNase1 was confirmed as the major nuclease performing dsRNA degradation. After a knockdown of the TcdsRNase1 nuclease activity, RNAi efficiency improved from 38.6% to 58.9% and from 20.9% to 53.9% for injection and ingestion of dsRNA, respectively. Our results contribute to a comprehensive understanding of the mechanisms influencing dsRNA stability and even RNAi efficiency in T. castaneum and point to a good method for improving RNAi efficiency through downregulation of the relevant nuclease activity.
英文关键词	Tribolium castaneum DNA/RNA non-specific endonuclease dsRNase RNA interference RNAi
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000572388600002
WOS关键词	COLORADO POTATO BEETLE ; LEPTINOTARSA-DECEMLINEATA ; DSRNA DEGRADATION ; CROP PROTECTION ; NEXT-GENERATION ; DESERT LOCUST ; INSECT PEST ; PEA APHID ; EXPRESSION ; GENE
WOS类目	Biochemistry & Molecular Biology ; Entomology
WOS研究方向	Biochemistry & Molecular Biology ; Entomology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/326475
作者单位	[Wang, Kangxu; Chen, Jiasheng; Wang, Jinda; Zhang, Hainan; Ze, Longji; Zhao, Chunqing; Han, Zhaojun] Nanjing Agr Univ, Coll Plant Protect, Dept Entomol, Agr Minist,Key Lab Monitoring & Management Plant, Nanjing 210095, Jiangsu, Peoples R China; [Peng, Yingchuan; Xiao, Haijun] Jiangxi Agr Univ, Inst Entomol, Nanchang 330045, Jiangxi, Peoples R China; [Zhu, Guanheng] Univ Kentucky, Coll Agr Food & Environm, Dept Entomol, Lexington, KY 40546 USA
推荐引用方式 GB/T 7714	Peng, Yingchuan,Wang, Kangxu,Chen, Jiasheng,et al. Identification of a double-stranded RNA-degrading nuclease influencing both ingestion and injection RNA interference efficiency in the red flour beetle Tribolium castaneum[J],2020,125.
APA	Peng, Yingchuan.,Wang, Kangxu.,Chen, Jiasheng.,Wang, Jinda.,Zhang, Hainan.,...&Han, Zhaojun.(2020).Identification of a double-stranded RNA-degrading nuclease influencing both ingestion and injection RNA interference efficiency in the red flour beetle Tribolium castaneum.INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY,125.
MLA	Peng, Yingchuan,et al."Identification of a double-stranded RNA-degrading nuclease influencing both ingestion and injection RNA interference efficiency in the red flour beetle Tribolium castaneum".INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY 125(2020).