干旱区生态环境知识资源中心(Arid): Discovery and validation of candidate SNP markers associated to heat stress response in pregnant ewes managed inside a climate-controlled chamber

Arid

DOI	10.1007/s11250-020-02379-3
	Discovery and validation of candidate SNP markers associated to heat stress response in pregnant ewes managed inside a climate-controlled chamber
	Luna-Nevarez, Guillermo; Kelly, Amy C.; Camacho, Leticia E.; Limesand, Sean W.; Reyna-Granados, Javier R.; Luna-Nevarez, Pablo
通讯作者	Luna-Nevarez, P
来源期刊	TROPICAL ANIMAL HEALTH AND PRODUCTION
ISSN	0049-4747
EISSN	1573-7438
出版年	2020
卷号	52 期号:6 页码:3457-3466
英文摘要	Sheep production in desert environments during summer is challenging due to heat stress which reduces feed intake, growth, and fertility. Despite warm conditions, some ewes are able to maintain a normal performance suggesting the existence of genetic bases underlying heat tolerance. Our objective was to discover and validate genetic markers associated with thermo-tolerance in pregnant ewes exposed to warm environmental conditions. Using a well-defined model laboratory of heat stress in sheep, pregnant Columbia-Rambouillet crossbred ewes (n = 100) were examined. Following acclimation to the laboratory at thermo-neutral conditions, heat stress was induced in ewes by increasing the temperature-humidity index in a control environmental chamber during mid-gestation. Feed intake, water consumption, and rectal temperature were recorded daily and used to establish the heat stress tolerance index (HSTI) for each ewe. Rectal temperature was a predictor (P < 0.05) of feed intake, and the regression coefficient was used to classify the HSTI. In a subset of 24 ewes, a genome-wide association study (GWAS) was performed using the Illumina OvineSNP50 BeadChip. Single-marker analysis detected 3 intragenic SNPs associated with HSTI (Pvalue = 10(-5)). Bayesian multi-marker approach discovered 26 chromosomal regions across the genome which accounted for 9.8% of the variation associated with HSTI. In an independent sheep population (n = 42), the three discovered SNPs were validated as molecular markers associated with thermo-tolerance phenotypic traits. These SNPs were located within the genes F13A1, PAM, and PRELID2. In conclusion, three SNPs appear to be novel molecular markers associated with heat stress tolerance in pregnant ewes providing new knowledge about genetic foundations of thermo-tolerance.
英文关键词	Ewes Heat stress SNP markers Thermo-tolerance
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000571095400001
WOS关键词	PLACENTAL INSUFFICIENCY ; IMPACT ; SHEEP
WOS类目	Agriculture, Dairy & Animal Science ; Veterinary Sciences
WOS研究方向	Agriculture ; Veterinary Sciences
来源机构	University of Arizona
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/326379
作者单位	[Luna-Nevarez, Guillermo; Reyna-Granados, Javier R.; Luna-Nevarez, Pablo] Inst Tecnol Sonora, Dept Ciencias Agron & Vet, Obregon 85000, Sonora, Mexico; [Kelly, Amy C.; Camacho, Leticia E.; Limesand, Sean W.] Univ Arizona, Sch Anim & Comparat Biomed Sci, Tucson, AZ 85721 USA
推荐引用方式 GB/T 7714	Luna-Nevarez, Guillermo,Kelly, Amy C.,Camacho, Leticia E.,et al. Discovery and validation of candidate SNP markers associated to heat stress response in pregnant ewes managed inside a climate-controlled chamber[J]. University of Arizona,2020,52(6):3457-3466.
APA	Luna-Nevarez, Guillermo,Kelly, Amy C.,Camacho, Leticia E.,Limesand, Sean W.,Reyna-Granados, Javier R.,&Luna-Nevarez, Pablo.(2020).Discovery and validation of candidate SNP markers associated to heat stress response in pregnant ewes managed inside a climate-controlled chamber.TROPICAL ANIMAL HEALTH AND PRODUCTION,52(6),3457-3466.
MLA	Luna-Nevarez, Guillermo,et al."Discovery and validation of candidate SNP markers associated to heat stress response in pregnant ewes managed inside a climate-controlled chamber".TROPICAL ANIMAL HEALTH AND PRODUCTION 52.6(2020):3457-3466.