干旱区生态环境知识资源中心(Arid): The first bioactivity studies of Acantholimon lycopodioides from high altitude Karakoram-Himalayan desert

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DOI	10.1016/j.sjbs.2020.04.006
	The first bioactivity studies of Acantholimon lycopodioides from high altitude Karakoram-Himalayan desert
	Parvez, Mohammad K.; Basudan, Omar A.; Noman, Omar M.; Al-Dosari, Mohammed S.; Alqasoumi, Saleh, I
通讯作者	Parvez, MK
来源期刊	SAUDI JOURNAL OF BIOLOGICAL SCIENCES
ISSN	1319-562X
EISSN	2213-7106
出版年	2020
卷号	27 期号:10 页码:2514-2520
英文摘要	Couple of ethnopharmacological surveys in the Indian Ladakh and Pakistani Shigar valleys has reported the medicinal use of Acantholimon lycopodioides against cardiac and gastric disorders that however, remains without scientific rationale or experimental validations. Here, we assess the in vitro bio/therapeutic activities of A. lycopodioides extracts as well as chloroform, ethyl acetate, n-butanol and aqueous fractions. The in vitro beta-carotene-linoleic acid bleaching and DPPH radical scavenging methods demonstrated a very high anti-oxidative property of chloroform and ethyl acetate fractions compared to others. Cell viability assay (MTT) on human cervical (HeLa), breast (MDA-MB321) and liver (HepG2) cancer cells revealed their differential cytotoxicity, except the chloroform fraction. Of these, the precipitate exerted highest cytotoxicity on HepG2 cells followed by aqueous fraction on MDA-MB321 cells. Notably, the non-cytotoxicity of chloroform fraction coincided with its highest anti-oxidative activity. Further, the chloroform fraction showed marked hepatoprotection (up to 84%) against 3'7'dichlorofluorescin triggered free radicals induced oxidative damage. Also, the hepatoprotective chloroform fraction mildly activated CYP3A4 in HepG2 cells (dual-luciferase assay). Moreover, the A. lycopodioides extracts and fractions showed differential anti-bacterial and anti-fungal activities. Of these, while S. aureus was more sensitive to the water-insoluble extract, ethyl acetate fraction showed moderate activity against E. coli and C. albicans. On the other hand, the chloroform fraction showed promising activity against S. Aureus, C. albicans, P. vulgaris and E. faecalis. In conclusion, our data for the first time, demonstrated promising anti-oxidative, hepatoprotective, anti-cancer, anti-microbial and CYP3A4 activating salutations of A. lycopodioides. This warrants further studies towards isolation and identification of its therapeutically active principles. (C) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.
英文关键词	Acantholimon lycopodioides Plumbaginaceae Anti-oxidant Anti-cancer Anti-microbial Cytochrome 450
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000570858800003
WOS关键词	MEDICINAL-PLANTS ; PLUMBAGINACEAE ; BOISS.
WOS类目	Biology
WOS研究方向	Life Sciences & Biomedicine - Other Topics
来源机构	King Saud University
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/326370
作者单位	[Parvez, Mohammad K.; Basudan, Omar A.; Noman, Omar M.; Al-Dosari, Mohammed S.; Alqasoumi, Saleh, I] King Saud Univ, Coll Pharm, Dept Pharmacognosy, Riyadh 11451, Saudi Arabia
推荐引用方式 GB/T 7714	Parvez, Mohammad K.,Basudan, Omar A.,Noman, Omar M.,et al. The first bioactivity studies of Acantholimon lycopodioides from high altitude Karakoram-Himalayan desert[J]. King Saud University,2020,27(10):2514-2520.
APA	Parvez, Mohammad K.,Basudan, Omar A.,Noman, Omar M.,Al-Dosari, Mohammed S.,&Alqasoumi, Saleh, I.(2020).The first bioactivity studies of Acantholimon lycopodioides from high altitude Karakoram-Himalayan desert.SAUDI JOURNAL OF BIOLOGICAL SCIENCES,27(10),2514-2520.
MLA	Parvez, Mohammad K.,et al."The first bioactivity studies of Acantholimon lycopodioides from high altitude Karakoram-Himalayan desert".SAUDI JOURNAL OF BIOLOGICAL SCIENCES 27.10(2020):2514-2520.