干旱区生态环境知识资源中心(Arid): Precocious Downregulation of Kruppel-Homolog 1 in the Migratory Locust,Locusta migratoria, Gives Rise to An Adultoid Phenotype with Accelerated Ovarian Development but Disturbed Mating and Oviposition

Arid

DOI	10.3390/ijms21176058
	Precocious Downregulation of Kruppel-Homolog 1 in the Migratory Locust,Locusta migratoria, Gives Rise to An Adultoid Phenotype with Accelerated Ovarian Development but Disturbed Mating and Oviposition
	Gijbels, Marijke; Schellens, Sam; Schellekens, Tine; Bruyninckx, Evert; Marchal, Elisabeth; Vanden Broeck, Jozef
通讯作者	Marchal, E ; Vanden Broeck, J
来源期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
EISSN	1422-0067
出版年	2020
卷号	21 期号:17
英文摘要	Kruppel-homolog 1 (Kr-h1) is a zinc finger transcription factor maintaining the status quo in immature insect stages and promoting reproduction in adult insects through the transduction of the Juvenile Hormone (JH) signal. Knockdown studies have shown that precocious silencing ofKr-h1in the immature stages results in the premature development of adult features. However, the molecular characteristics and reproductive potential of these premature adult insect stages are still poorly understood. Here we report on an adult-like or 'adultoid' phenotype of the migratory locust,Locusta migratoria, obtained after a premature metamorphosis induced by the silencing ofLmKr-h1in the penultimate instar. The freshly molted adultoid shows precocious development of adult features, corresponding with increased transcript levels of the adult specifier geneLmE93. Furthermore, accelerated ovarian maturation and vitellogenesis were observed in female adultoids, coinciding with elevated expression ofLmCYP15A1incorpora allata(CA) andLmKr-h1andvitellogeningenes (LmVg) in fat body, whereasLmE93andMethoprene-tolerant(LmMet) transcript levels decreased in fat body. In adultoid ovaries, expression of theHalloweengenes,Spook(LmSpo) andPhantom(LmPhm), was elevated as well. In addition, the processes of mating and oviposition were severely disturbed in these females.L. migratoriais a well-known, swarm-forming pest insect that can destroy crops and harvests in some of the world's poorest countries. As such, a better understanding of factors that are capable of significantly reducing the reproductive potential of this pest may be of crucial importance for the development of novel locust control strategies.
英文关键词	ecdysteroid Hemimetabola insect juvenile hormone metamorphosis reproduction
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000569683200001
WOS关键词	RED FLOUR BEETLE ; ACID-O-METHYLTRANSFERASE ; JUVENILE-HORMONE ACTION ; METHOPRENE-TOLERANT ; FOLLICLE CELLS ; LOCUSTA-MIGRATORIA ; DESERT LOCUST ; DROSOPHILA-MELANOGASTER ; KRUPPEL-HOMOLOG-1 KR-H1 ; RNA INTERFERENCE
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/326318
作者单位	[Gijbels, Marijke; Schellens, Sam; Schellekens, Tine; Bruyninckx, Evert; Marchal, Elisabeth; Vanden Broeck, Jozef] Katholieke Univ Leuven, Zool Inst, Res Grp Mol Dev Physiol & Signal Transduct, Naamsestr 59 Box 2465, B-3000 Leuven, Belgium; [Marchal, Elisabeth] Imec, Life Sci Technol, Kapeldreef 75, B-3001 Leuven, Belgium
推荐引用方式 GB/T 7714	Gijbels, Marijke,Schellens, Sam,Schellekens, Tine,et al. Precocious Downregulation of Kruppel-Homolog 1 in the Migratory Locust,Locusta migratoria, Gives Rise to An Adultoid Phenotype with Accelerated Ovarian Development but Disturbed Mating and Oviposition[J],2020,21(17).
APA	Gijbels, Marijke,Schellens, Sam,Schellekens, Tine,Bruyninckx, Evert,Marchal, Elisabeth,&Vanden Broeck, Jozef.(2020).Precocious Downregulation of Kruppel-Homolog 1 in the Migratory Locust,Locusta migratoria, Gives Rise to An Adultoid Phenotype with Accelerated Ovarian Development but Disturbed Mating and Oviposition.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,21(17).
MLA	Gijbels, Marijke,et al."Precocious Downregulation of Kruppel-Homolog 1 in the Migratory Locust,Locusta migratoria, Gives Rise to An Adultoid Phenotype with Accelerated Ovarian Development but Disturbed Mating and Oviposition".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21.17(2020).