干旱区生态环境知识资源中心(Arid): Cross genera transferability of microsatellite markers from other members of family Bignoniaceae toTecomella undulata(Sm.) Seem

Arid

DOI	10.1007/s11738-020-03138-5
	Cross genera transferability of microsatellite markers from other members of family Bignoniaceae toTecomella undulata(Sm.) Seem
	Kalia, Rajwant K.; Chhajer, Sidhika; Pathak, Rakesh
通讯作者	Kalia, RK
来源期刊	ACTA PHYSIOLOGIAE PLANTARUM
ISSN	0137-5881
EISSN	1861-1664
出版年	2020
卷号	42 期号:9
英文摘要	Transferability of SSR markers from related species/genera is a rapid and cost-effective method to enhance genomic database of genetically neglected crops. Monotypic genusTecomella undulata, a pharmacologically important endangered timber tree, found in hyper arid regions is one such genetic resource limited species, wherein neither SSR identification nor cross transferability studies have been initiated. Transferability of 69 cross-genera SSR primers selected from other members of family bignoniaceae (Incarvillea sinensis,I. mairei, Jacaranda copaia,Tabebuia aureaandArrabidaea chica) showed 40.58% (28 primers) transferability amplifying 1-10 amplicons ranging in the size from 80to 600 bp in 24 accessions ofT. undulata. Per cent marker transferability varied from 66.67% (A. chica) to 38.89% (J. copaia) among the genera tested while no transferable markers could be identified fromI. sinensis. Transferability of simple di-/tri-nucleotide repeat (34.2-37.5%) and complex nucleotide sequence (60.0%) based SSR motifs were higher as compared to penta-/hexa-nucleotide based repeat motifs. Within the 28 transferable markers, 26 (92.86%) were polymorphic while 2 (7.14%) were monomorphic. Average number of alleles per primer was 5.39 while average polymorphic alleles per locus were 5.11. The PIC values of SSR markers ranged from 0.23 to 0.85 with an average of 0.59. Efficiency of the identified cross species SSR markers was tested for diversity analysis using a set of 24 trees (8 each of yellow, orange and red flower colour morphotypes). The 24 samples clustered into six groups with similarity coefficient ranging from 0.29 to 0.67, while six accessions formed distinct out groups. Distribution of accessions in the SSR dendrogram showed no correlation with flower colour. More diversity (99%) was observed within populations compared to that existing between populations (1%). The present study can enrich the genomic background ofT. undulataby identifying suitable polymorphic markers from confamiliar species that can be applied for genetic diversity studies, mapping of QTLs and cultivar identification as well.
英文关键词	Arid region Endangered tree Cross-transferability Rohida SSR markers
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000569242700001
WOS关键词	REPEAT SSR MARKERS ; TECOMELLA-UNDULATA ; GENETIC DIVERSITY ; TREE ; LOCI ; POLYMORPHISMS ; WHEAT ; L.
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
来源机构	ICAR Central Arid Zone Research Institute
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/326286
作者单位	[Kalia, Rajwant K.; Chhajer, Sidhika; Pathak, Rakesh] Cent Arid Zone Res Inst CAZRI, Div Plant Improvement & Pest Management, Jodhpur 342003, Rajasthan, India
推荐引用方式 GB/T 7714	Kalia, Rajwant K.,Chhajer, Sidhika,Pathak, Rakesh. Cross genera transferability of microsatellite markers from other members of family Bignoniaceae toTecomella undulata(Sm.) Seem[J]. ICAR Central Arid Zone Research Institute,2020,42(9).
APA	Kalia, Rajwant K.,Chhajer, Sidhika,&Pathak, Rakesh.(2020).Cross genera transferability of microsatellite markers from other members of family Bignoniaceae toTecomella undulata(Sm.) Seem.ACTA PHYSIOLOGIAE PLANTARUM,42(9).
MLA	Kalia, Rajwant K.,et al."Cross genera transferability of microsatellite markers from other members of family Bignoniaceae toTecomella undulata(Sm.) Seem".ACTA PHYSIOLOGIAE PLANTARUM 42.9(2020).