干旱区生态环境知识资源中心(Arid): Acinetobacter baumannii NCIMB8209: a Rare Environmental Strain Displaying Extensive Insertion Sequence-Mediated Genome Remodeling Resulting in the Loss of Exposed Cell Structures and Defensive Mechanisms

Arid

DOI	10.1128/mSphere.00404-20
	Acinetobacter baumannii NCIMB8209: a Rare Environmental Strain Displaying Extensive Insertion Sequence-Mediated Genome Remodeling Resulting in the Loss of Exposed Cell Structures and Defensive Mechanisms
	Repizo, Guillermo D.; Espariz, Martin; Seravalle, Joana L.; Miloslavich, Juan Ignacio Diaz; Steimbruch, Bruno A.; Shuman, Howard A.; Viale, Alejandro M.
通讯作者	Repizo, GD ; Viale, AM
来源期刊	MSPHERE
ISSN	2379-5042
出版年	2020
卷号	5 期号:4
英文摘要	Acinetobacter baumannii represents nowadays an important nosocomial pathogen of poorly defined reservoirs outside the clinical setting. Here, we con-ducted whole-genome sequencing analysis of the Acinetobacter sp. NCIMB8209 col-lection strain, isolated in 1943 from the aerobic degradation (retting) of desert gu-ayule shrubs. Strain NCIMB8209 contained a 3.75-Mb chromosome and a plasmid of 134 kb. Phylogenetic analysis based on core genes indicated NCIMB8209 affiliation to A. baumannii, a result supported by the identification of a chromosomal blaOXA-51-like gene. Seven genomic islands lacking antimicrobial resistance determinants, 5 regions encompassing phage-related genes, and notably, 93 insertion sequences (IS) were found in this genome. NCIMB8209 harbors most genes linked to persistence and virulence described in contemporary A. baumannii clinical strains, but many of the genes encoding components of surface structures are interrupted by IS. More -over, defense genetic islands against biological aggressors such as type 6 secretion systems or CRISPR-cas are absent from this genome. These findings correlate with a low capacity of NCIMB8209 to form biofilm and pellicle, low motility on semisolid medium, and low virulence toward Galleria mellonella and Caenorhabditis elegans. Searching for catabolic genes and concomitant metabolic assays revealed the ability of NCIMB8209 to grow on a wide range of substances produced by plants, including aromatic acids and defense compounds against external aggressors. All the above features strongly suggest that NCIMB8209 has evolved specific adaptive features to a particular environmental niche. Moreover, they also revealed that the remarkable genetic plasticity identified in contemporary A. baumannii clinical strains represents an intrinsic characteristic of the species. IMPORTANCE Acinetobacter baumannii is an ESKAPE (Enterococcus faecium, Staphylo-coccus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aerugi-nosa, and Enterobacter species) opportunistic pathogen, with poorly defined natural habitats/reservoirs outside the clinical setting. A. baumannii arose from the Acineto-bacter calcoaceticus-A. baumannii complex as the result of a population bottleneck, followed by a recent population expansion from a few clinically relevant clones en-dowed with an arsenal of resistance and virulence genes. Still, the identification of virulence traits and the evolutionary paths leading to a pathogenic lifestyle has re-mained elusive, and thus, the study of nonclinical ("environmental") A. baumannii isolates is necessary. We conducted here comparative genomic and virulence studies on A. baumannii NCMBI8209 isolated in 1943 from the microbiota responsible for the decomposition of guayule, and therefore well differentiated both temporally and epidemiologically from the multidrug-resistant strains that are predominant nowa-days. Our work provides insights on the adaptive strategies used by A. baumannii to escape from host defenses and may help the adoption of measures aimed to limit its further dissemination.
英文关键词	environmental Acinetobacter baumannii preantibiotic-era Acinetobacter baumannii environmental reservoirs comparative genomics insertion sequences virulence factors
类型	Article
语种	英语
开放获取类型	DOAJ Gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000568743200018
WOS关键词	PHENYLACETIC ACID ; SECRETION SYSTEM ; CAPSULAR POLYSACCHARIDE ; SP NOV. ; RESISTANCE ; IDENTIFICATION ; PHAGE ; GENE ; CATABOLISM ; DIVERSITY
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/326224
作者单位	[Repizo, Guillermo D.; Espariz, Martin; Seravalle, Joana L.; Miloslavich, Juan Ignacio Diaz; Steimbruch, Bruno A.; Viale, Alejandro M.] Univ Nacl Rosario, Inst Biol Mol & Celular Rosario IBR, Fac Ciencias Bioquim & Farmaceut, Dept Microbiol,CONICET, Rosario, Argentina; [Shuman, Howard A.] Univ Chicago, Dept Microbiol, Chicago, IL 60637 USA
推荐引用方式 GB/T 7714	Repizo, Guillermo D.,Espariz, Martin,Seravalle, Joana L.,et al. Acinetobacter baumannii NCIMB8209: a Rare Environmental Strain Displaying Extensive Insertion Sequence-Mediated Genome Remodeling Resulting in the Loss of Exposed Cell Structures and Defensive Mechanisms[J],2020,5(4).
APA	Repizo, Guillermo D..,Espariz, Martin.,Seravalle, Joana L..,Miloslavich, Juan Ignacio Diaz.,Steimbruch, Bruno A..,...&Viale, Alejandro M..(2020).Acinetobacter baumannii NCIMB8209: a Rare Environmental Strain Displaying Extensive Insertion Sequence-Mediated Genome Remodeling Resulting in the Loss of Exposed Cell Structures and Defensive Mechanisms.MSPHERE,5(4).
MLA	Repizo, Guillermo D.,et al."Acinetobacter baumannii NCIMB8209: a Rare Environmental Strain Displaying Extensive Insertion Sequence-Mediated Genome Remodeling Resulting in the Loss of Exposed Cell Structures and Defensive Mechanisms".MSPHERE 5.4(2020).