干旱区生态环境知识资源中心(Arid): Cutting the Gordian knot: early and complete amino acid sequence confirmation of class II lasso peptides by HCD fragmentation

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DOI	10.1038/s41429-020-00369-z
	Cutting the Gordian knot: early and complete amino acid sequence confirmation of class II lasso peptides by HCD fragmentation
	Jarmusch, Scott A.; Feldmann, Ingo; Blank-Landeshammer, Bernhard; Cortes-Albayay, Carlos; Franco Castro, Jean; Andrews, Barbara; Asenjo, Juan A.; Sickmann, Albert; Ebel, Rainer; Jaspars, Marcel
通讯作者	Jaspars, M
来源期刊	JOURNAL OF ANTIBIOTICS
ISSN	0021-8820
EISSN	1881-1469
出版年	2020
卷号	73 期号:11 页码:772-779
英文摘要	Lasso peptides are a diverse class of ribosomally synthesized and post-translationally modified peptides (RiPPs). Their proteolytic and thermal stability alongside their growing potential as therapeutics has increased attention to these antimicrobial peptides. With the advent of genome mining, the discovery of RiPPs allows for the accurate prediction of putatively encoded structures, however, MS(n)experiments only provide partial sequence confirmation, therefore 2D NMR experiments are necessary for characterisation. Multiple MS/MS techniques were applied to two structurally characterized lasso peptides, huascopeptin and leepeptin, and one uncharacterized lasso peptide, citrulassin C, which was not isolable in sufficient quantity for NMR analysis. We have shown that MS(2)can be used to elucidate the full amino acid sequences previously predicted with genome mining for this compound class. HCD was able to open the macrocycles and fragment the newly opened linear peptides, confirming the complete amino acid sequences of the characterised lasso peptides. In addition, to determine if this technique could be applied at the earliest stages of the isolation process, we targeted a lasso peptide found by genome mining, citrulassin C, and were able to fully elucidate the amino acid sequence using only MS(2)from a semi-crude extract ofStreptomyces huasconensisHST28(T).
类型	Article
语种	英语
开放获取类型	Green Accepted
收录类别	SCI-E
WOS记录号	WOS:000567725400001
WOS关键词	CYCLIC-PEPTIDES ; MICROCIN J25 ; ANTAGONIST ; BI-32169 ; DESERT ; TOOL
WOS类目	Biotechnology & Applied Microbiology ; Immunology ; Microbiology ; Pharmacology & Pharmacy
WOS研究方向	Biotechnology & Applied Microbiology ; Immunology ; Microbiology ; Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/326165
作者单位	[Jarmusch, Scott A.; Franco Castro, Jean; Ebel, Rainer; Jaspars, Marcel] Univ Aberdeen, Dept Chem, Marine Biodiscovery Ctr, Aberdeen AB24 3UE, Scotland; [Feldmann, Ingo; Blank-Landeshammer, Bernhard; Cortes-Albayay, Carlos; Sickmann, Albert] Leibniz Inst Analyt Wissensch ISAS eV, Dortmund, Germany; [Franco Castro, Jean; Andrews, Barbara; Asenjo, Juan A.] Univ Chile, Ctr Biotechnol & Bioengn, Beauchef 851, Santiago, Chile; [Franco Castro, Jean] John Innes Ctr, Dept Mol Microbiol, Norwich NR4 7UH, Norfolk, England; [Jarmusch, Scott A.] Uppsala Univ, Dept Pharmaceut Biosci Pharmacognosy, Uppsala, Sweden; [Franco Castro, Jean] Inst Invest Agr INIA, Chillan, Chile
推荐引用方式 GB/T 7714	Jarmusch, Scott A.,Feldmann, Ingo,Blank-Landeshammer, Bernhard,et al. Cutting the Gordian knot: early and complete amino acid sequence confirmation of class II lasso peptides by HCD fragmentation[J],2020,73(11):772-779.
APA	Jarmusch, Scott A..,Feldmann, Ingo.,Blank-Landeshammer, Bernhard.,Cortes-Albayay, Carlos.,Franco Castro, Jean.,...&Jaspars, Marcel.(2020).Cutting the Gordian knot: early and complete amino acid sequence confirmation of class II lasso peptides by HCD fragmentation.JOURNAL OF ANTIBIOTICS,73(11),772-779.
MLA	Jarmusch, Scott A.,et al."Cutting the Gordian knot: early and complete amino acid sequence confirmation of class II lasso peptides by HCD fragmentation".JOURNAL OF ANTIBIOTICS 73.11(2020):772-779.