干旱区生态环境知识资源中心(Arid): Detection of azithromycin residue in broiler feathers by liquid chromatography-tandem mass spectrometry

Arid

DOI	10.1016/j.jchromb.2020.122225
	Detection of azithromycin residue in broiler feathers by liquid chromatography-tandem mass spectrometry
	Xiang, Jian Jun; Xi, Ci Dan Zha; Liao, Qie Gen; Yuan, Li Juan; Zhang, Da Wen
通讯作者	Liao, QG
来源期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN	1570-0232
EISSN	1873-376X
出版年	2020
卷号	1152
英文摘要	In the study, a sensitive and reproducible method for the quantitative analysis of azithromycin in broiler feather samples by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed. Feather samples were rinsed after being wrapped in medical gauze, then chopped and then added to 5% (v/v) ammonia in methanol solution for ultrasonic extraction. The extract was purified by the combination of commercial polymeric microparticles (Oasis MCX) and Fe3O4 nanoparticles. The LC separation was performed on an Agilent Eclipse plus C18 column. Multiple reaction monitoring was used for the selective detection of azithromycin. The good linearity curve of azithromycin in feather sample was in the range from 1.0 mu g kg(-1) to 100.0 mu g kg(-1) with 0.9935 of correlation coefficient. And the limit detection and limit of quantification was 0.5 mu g kg(-1) and 2.0 mu g kg(-1) in spiked feather samples. The recoveries of azithromycin were 85.2-94.7% with the relative standard deviation less than 10%. The established method is simple, rapid, sensitive and specific, and could meet the need of government and enterprises to monitor the illegal use of azithromycin in livestock and poultry breeding.
英文关键词	Azithromycin Broiler feather MCX@Fe3O4 nanoparticles LC-MS/MS
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000567378700015
WOS关键词	MAGNETIC NANOPARTICLES ; HUMAN PLASMA ; HPLC METHOD ; RP-HPLC ; HAIR ; VALIDATION ; EXTRACTION ; STEROIDS ; ESTERS
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/326137
作者单位	[Xiang, Jian Jun; Liao, Qie Gen; Yuan, Li Juan; Zhang, Da Wen] Jiangxi Acad Agr Sci, Agr Prod Qual Safety & Stand Inst, Nanchang 330200, Jiangxi, Peoples R China; [Xi, Ci Dan Zha] Tibet Acad Agr & Anim Husb Sci, Inst Agr Qual Stand & Testing, Lhasa 850000, Peoples R China
推荐引用方式 GB/T 7714	Xiang, Jian Jun,Xi, Ci Dan Zha,Liao, Qie Gen,et al. Detection of azithromycin residue in broiler feathers by liquid chromatography-tandem mass spectrometry[J],2020,1152.
APA	Xiang, Jian Jun,Xi, Ci Dan Zha,Liao, Qie Gen,Yuan, Li Juan,&Zhang, Da Wen.(2020).Detection of azithromycin residue in broiler feathers by liquid chromatography-tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1152.
MLA	Xiang, Jian Jun,et al."Detection of azithromycin residue in broiler feathers by liquid chromatography-tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1152(2020).