干旱区生态环境知识资源中心(Arid): Determination of antipsychotic drugs in nails and hair by liquid chromatography tandem mass spectrometry and evaluation of their incorporation into keratinized matrices

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DOI	10.1016/j.jpba.2020.113443
	Determination of antipsychotic drugs in nails and hair by liquid chromatography tandem mass spectrometry and evaluation of their incorporation into keratinized matrices
	Cobo-Golpe, Maria; de-Castro-Rios, Ana; Cruz, Angelines; Paramo, Mario; Lopez-Rivadulla, Manuel; Lendoiro, Elena
通讯作者	Lendoiro, E
来源期刊	JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
ISSN	0731-7085
EISSN	1873-264X
出版年	2020
卷号	189
英文摘要	Nail samples are an alternative to hair for long-term monitoring of drug use, although there are a limited number of studies about its applicability. This study presents the development and validation of a LC-MS/MS method for the determination of five antipsychotic drugs (clozapine, haloperidol, levomepromazine, olanzapine and quetiapine) in nail and in hair samples. Samples were washed with dichloromethane, pulverized with a ball mill, and incubated in water:acetonitrile (50:50 v/v) with horizontal agitation for 90 min. Then, samples were purified by solid phase extraction with OASIS MCX cartridges and analysed by LC-MS/MS. The analytical method was fully validated in nails and in hair, including: limits of detection (2.5 pg/mg and 2.5-10 pg/mg, respectively), limits of quantification (LOQ) (10 pg/mg and 10-20 pg/mg, respectively), linearity (LOQto 10,000 pg/mg), selectivity (no endogenous or exogenous interferences), accuracy (97.4-106.9% and 97.3-108.2%, respectively), imprecision (<7.9% and <8.6%, respectively), extraction efficiency (62.3%-109.8% and 45.1%-83.6%, respectively), matrix effect (-35.6% to 654.4% and -71.0% to -10.8%, respectively) and autosampler stability after 72 h (%loss <12.4%). Moreover, paired fingernail, toenail and hair samples from 13 patients under chronic treatment were analysed, and concentrations in the different matrices were compared. Concentrations in real samples ranged from 11.3-8306 pg/mg in fingernails, from 12.7-1755.4 pg/mg in toenails and from 17.6-24045.4 pg/mg in hair. Hair concentrations were generally higher than nail concentrations; however, a variable pattern was found in fingernails and toenails depending on the case. In addition, concentrations in paired hair and nail samples from a patient under chronic treatment with quetiapine at different doses were studied for 1-year. (C) 2020 Elsevier B.V. All rights reserved.
英文关键词	Nails Hair Antipsychotics LC-MS/MS
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000566411100014
WOS关键词	INDIVIDUAL DOSAGE HISTORY ; HUMAN SCALP HAIR ; FINGERNAIL CLIPPINGS ; ALTERNATIVE MATRIX ; HALOPERIDOL ; COCAINE ; QUANTIFICATION ; ZOLPIDEM ; ABUSE ; BENZODIAZEPINES
WOS类目	Chemistry, Analytical ; Pharmacology & Pharmacy
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/326064
作者单位	[Cobo-Golpe, Maria; de-Castro-Rios, Ana; Cruz, Angelines; Lopez-Rivadulla, Manuel; Lendoiro, Elena] Univ Santiago de Compostela, Inst Ciencias Forenses, Serv Toxicol, San Francisco S-N, Santiago De Compostela 15782, Spain; [Paramo, Mario] Complexo Hosp Univ Santiago CHUS, Serv Psiquiatria, Serv Galego Saude SERGAS, Santiago De Compostela, Spain
推荐引用方式 GB/T 7714	Cobo-Golpe, Maria,de-Castro-Rios, Ana,Cruz, Angelines,et al. Determination of antipsychotic drugs in nails and hair by liquid chromatography tandem mass spectrometry and evaluation of their incorporation into keratinized matrices[J],2020,189.
APA	Cobo-Golpe, Maria,de-Castro-Rios, Ana,Cruz, Angelines,Paramo, Mario,Lopez-Rivadulla, Manuel,&Lendoiro, Elena.(2020).Determination of antipsychotic drugs in nails and hair by liquid chromatography tandem mass spectrometry and evaluation of their incorporation into keratinized matrices.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,189.
MLA	Cobo-Golpe, Maria,et al."Determination of antipsychotic drugs in nails and hair by liquid chromatography tandem mass spectrometry and evaluation of their incorporation into keratinized matrices".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 189(2020).