干旱区生态环境知识资源中心(Arid): Immune and sex-biased gene expression in the threatened Mojave desert tortoise, Gopherus agassizii

Arid

DOI	10.1371/journal.pone.0238202
	Immune and sex-biased gene expression in the threatened Mojave desert tortoise, Gopherus agassizii
	Xu, Cindy; Dolby, Greer A.; Drake, K. Kristina; Esque, Todd C.; Kusumi, Kenro
通讯作者	Kusumi, K
来源期刊	PLOS ONE
ISSN	1932-6203
出版年	2020
卷号	15 期号:8
英文摘要	The immune system of ectotherms, particularly non-avian reptiles, remains poorly characterized regarding the genes involved in immune function, and their function in wild populations. We used RNA-Seq to explore the systemic response of Mojave desert tortoise (Gopherus agassizii) gene expression to three levels ofMycoplasmainfection to better understand the host response to this bacterial pathogen. We found over an order of magnitude more genes differentially expressed between male and female tortoises (1,037 genes) than differentially expressed among immune groups (40 genes). There were 8 genes differentially expressed among both variables that can be considered sex-biased immune genes in this tortoise. Among experimental immune groups we find enriched GO biological processes for cysteine catabolism, regulation of type 1 interferon production, and regulation of cytokine production involved in immune response. Sex-biased transcription involves iron ion transport, iron ion homeostasis, and regulation of interferon-beta production to be enriched. More detailed work is needed to assess the seasonal response of the candidate genes found here. How seasonal fluctuation of testosterone and corticosterone modulate the immunosuppression of males and their susceptibility toMycoplasmainfection also warrants further investigation, as well as the importance of iron in the immune function and sex-biased differences of this species. Finally, future transcriptional studies should avoid drawing blood from tortoises via subcarapacial venipuncture as the variable aspiration of lymphatic fluid will confound the differential expression of genes.
类型	Article
语种	英语
开放获取类型	DOAJ Gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000566205500023
WOS关键词	RESPIRATORY-TRACT DISEASE ; MYCOPLASMA-AGASSIZII ; ALPHA PRODUCTION ; GENDER ; DIMORPHISM ; LIPOPOLYSACCHARIDE ; HORMONES ; BLOOD ; REPLICATION ; MACROPHAGE
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
来源机构	United States Geological Survey ; Arizona State University
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/326042
作者单位	[Xu, Cindy; Dolby, Greer A.; Kusumi, Kenro] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA; [Dolby, Greer A.] Arizona State Univ, Ctr Mech Evolut, Biodesign Inst, Tempe, AZ USA; [Drake, K. Kristina; Esque, Todd C.] US Geol Survey, Western Ecol Res Ctr, Henderson, NV 89074 USA
推荐引用方式 GB/T 7714	Xu, Cindy,Dolby, Greer A.,Drake, K. Kristina,et al. Immune and sex-biased gene expression in the threatened Mojave desert tortoise, Gopherus agassizii[J]. United States Geological Survey, Arizona State University,2020,15(8).
APA	Xu, Cindy,Dolby, Greer A.,Drake, K. Kristina,Esque, Todd C.,&Kusumi, Kenro.(2020).Immune and sex-biased gene expression in the threatened Mojave desert tortoise, Gopherus agassizii.PLOS ONE,15(8).
MLA	Xu, Cindy,et al."Immune and sex-biased gene expression in the threatened Mojave desert tortoise, Gopherus agassizii".PLOS ONE 15.8(2020).